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Beyond Crispr:Single Base Editors for Human Health and Crop Improvement


Affiliations
1 Department of Genetics and Plant Breeding, Ch. Charan Singh University, Meerut - 250 004, India
 

During 2016–2018, CRISPR/Cas9 technology was modified using disabled Cas9 with nickase activity in combination with cytosine/adenine deaminases for the development of four generations of cytosine base editors (BE1–BE4) for C → U conversion and at least seven generations of adenine base editors (ABE1–ABE7) for A → I conversion. These base editors exhibited improved efficiency and reduced frequency of deletions among the products. Further improvement in the form of enhanced base editors and high-fidelity base editors was achieved through the use of 1-3 copies of uracil N-glycosylase inhibitors and phage Mu-Gam protein. The technology will bring precision to gene editing technology for human healthcare and crop improvement.

Keywords

AID/APOBEC, Base Editing, CRISPR/Cas9, Cytidine/Adenine Deaminases, Target AID.
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  • Beyond Crispr:Single Base Editors for Human Health and Crop Improvement

Abstract Views: 383  |  PDF Views: 113

Authors

P. K. Gupta
Department of Genetics and Plant Breeding, Ch. Charan Singh University, Meerut - 250 004, India

Abstract


During 2016–2018, CRISPR/Cas9 technology was modified using disabled Cas9 with nickase activity in combination with cytosine/adenine deaminases for the development of four generations of cytosine base editors (BE1–BE4) for C → U conversion and at least seven generations of adenine base editors (ABE1–ABE7) for A → I conversion. These base editors exhibited improved efficiency and reduced frequency of deletions among the products. Further improvement in the form of enhanced base editors and high-fidelity base editors was achieved through the use of 1-3 copies of uracil N-glycosylase inhibitors and phage Mu-Gam protein. The technology will bring precision to gene editing technology for human healthcare and crop improvement.

Keywords


AID/APOBEC, Base Editing, CRISPR/Cas9, Cytidine/Adenine Deaminases, Target AID.

References





DOI: https://doi.org/10.18520/cs%2Fv116%2Fi3%2F386-397