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An Efficient Method for Acclimatization:in vitro Hardening of Tissue Culture-Raised Tea Plants (Camellia sinensis (l.) O. Kuntze)


Affiliations
1 G.B. Pant National Institute of Himalayan Environment and Sustainable Development, Kosi-Katarmal, Almora 263 643, India
2 Present address: Graphic Era Deemed to be University, Clement Town, Dehradun 248 002, India
 

In the 21st century while there is all-round development in every sphere of science and technology, a major limitation in micropropagation technology still remains; a large number of plantlets of several species die during hardening and ex vitro transfer. In the present study, introducing an in vitro incubation period in plant growth regulator (PGR)-free medium followed by ischolar_main induction of micropropagated tea shoots (Camellia sinensis (L.) O. Kuntze) and increasing it to 12 weeks instead of the usual 4 weeks gave excellent results. Secondly, cultures were grown in larger vessels, i.e. in 500 ml conical flasks in place of normal 250 ml conical flasks. Interestingly, prolonged incubation (12 weeks) significantly enhanced overall growth, i.e. length, stem girth, leaf number and leaf area of plantlets. The use of larger-sized culture flask also positively influenced growth. The abovementioned two simple modifications in culture conditions improved overall growth of plantlets. Also, plantlets raised through prolonged culture were acclimatized with 94% survival efficiency in comparison to 46% with normal culture period, and showed improved growth performance on transfer to soil in the nursery, irrespective of the culture vessel size.

Keywords

Acclimatization, in vitro Hardening, Lab-to-Land Transfer, Prolonged Culture, Tea Micropropagation.
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  • Bag, N., Palni, L. M. S., Chandra, S. and Nandi, S. K., Somatic embryogenesis in ‘maggar’ bamboo (Dendrocalamus hamiltonii) and field performance of regenerated plants. Curr. Sci., 2012, 102(9), 1279–1287.

  • Pandey, A., Palni, L. M. S. and Bag, N., Biological hardening of tissue culture raised tea plants through rhizosphere bacteria. Biotechnol. Lett., 2000, 22, 1087–1091.

  • Ziv, M., In vitro acclimatization. In Automation of Environmental Control in Plant Tissue Culture (eds Atiken-Christie J., Kozai, T. and Smith, M. L.), Kluwer, The Netherlands, 1995, pp. 493–516.

  • Bag, N., Palni, L. M. S. and Nandi, S. K., Mass propagation of tea using tissue culture methods. Physiol. Mol. Biol. Plants, 1997, 3, 99–103.

  • Kato, M., Regeneration of plants from tea stem callus. Jpn. J. Breed, 1985, 35, 317–322.

  • Akula, A. and Dood, W. A., Direct somatic embryogenesis in a selected teaclone, ‘TRI-2025’ (Camellia sinensi (L.) O. Kuntze) from nodal explants. Plant Cell Rep., 1998, 17, 804–809.

  • Wu, C. T., Studies on the tissue culture of tea plant. J. Agric. Assoc. China (Taiwan), 1976, 93, 30–42.

  • Banerjee, M. and Agarwal, B., In vitro ischolar_maining of tea (Camellia sinensis (L.) O. Kuntze). Indian J. Exp. Biol., 1990, 28(10), 936– 939.

  • Prakash, O., Sood, A., Sharma, M. and Ahuja, P. S., Grafting micropropagated tea (Camellia sinensis (L.) O. Kuntze) shoots on tea seedlings- a new approach to tea propagation. Plant Cell Rep., 1999, 18, 883–888.

  • Bag, N. and Palni, L. M. S., A two-step and highly reproducible procedure for in vitro ischolar_maining of micropropagated tea (Camellia sinensis L. (O) Kuntze) shoots. J. Hortic. Sci. Biotechnol., 2010, 85(3), 197–204.

  • Murashige, T. and Skoog, F., A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant., 1962, 15, 473–497.

  • Bag, N., Chandra, S., Palni, L. M. S. and Nandi, S. K., Micrpropropagation of Dev-ringal (Thamnocalamus spathiflorus [(Trin.) Munro] – a temperate bamboo, and comparison of in vitro propagated plants and seedlings. Plant Sci., 2000, 156, 125–135.

  • Dhawan, V. and Bhojwani, S. S., Hardening in vitro and morphophysiological changes in the leaves during acclimatization of micropropagated plants of Leucaena leucocephela (Lam.) de Wit. Plant Sci., 1987, 53, 65–72.

  • Chandra, S., Bandopadhyay, R., Kumar, V. and Chandra, R., Acclimatization of tissue cultured plantlets: from laboratory to land. J. Biotechnol. Lett., 2010, 32, 199–1205.

  • Cappelades, M., Fontarnau, R., Carulla, C. and Debergh, P., Environment influences anatomy of stomata and epidermal cells in tissue cultured Rosa multiflora. J. Am. Soc. Hortic. Sci., 1990, 115, 141–145.

  • Sutter, E., Chemical composition of epicuticular wax in cabbage plants grown in vitro. Can. J. Bot., 1984, 62, 74–77.

  • Kozai, T., Kino, S., Jeong, B. R., Kinowaki, M., Ochiai, M., Hayashi, M. and Mori, K., A sideward lighting system using diffusive optical fibers for production of vigorous micropropagated plantlets. Acta Hortic., 1992, 319, 237–242.

  • Deb, C. R. and Imchen, T., An efficient in vitro hardening technique of tissue culture raised plants. Biotechnology, 2010, 9(1), 79–83.


Abstract Views: 390

PDF Views: 129




  • An Efficient Method for Acclimatization:in vitro Hardening of Tissue Culture-Raised Tea Plants (Camellia sinensis (l.) O. Kuntze)

Abstract Views: 390  |  PDF Views: 129

Authors

Niladri Bag
G.B. Pant National Institute of Himalayan Environment and Sustainable Development, Kosi-Katarmal, Almora 263 643, India
Lok Man S. Palni
Present address: Graphic Era Deemed to be University, Clement Town, Dehradun 248 002, India
Shyamal Kumar Nandi
G.B. Pant National Institute of Himalayan Environment and Sustainable Development, Kosi-Katarmal, Almora 263 643, India

Abstract


In the 21st century while there is all-round development in every sphere of science and technology, a major limitation in micropropagation technology still remains; a large number of plantlets of several species die during hardening and ex vitro transfer. In the present study, introducing an in vitro incubation period in plant growth regulator (PGR)-free medium followed by ischolar_main induction of micropropagated tea shoots (Camellia sinensis (L.) O. Kuntze) and increasing it to 12 weeks instead of the usual 4 weeks gave excellent results. Secondly, cultures were grown in larger vessels, i.e. in 500 ml conical flasks in place of normal 250 ml conical flasks. Interestingly, prolonged incubation (12 weeks) significantly enhanced overall growth, i.e. length, stem girth, leaf number and leaf area of plantlets. The use of larger-sized culture flask also positively influenced growth. The abovementioned two simple modifications in culture conditions improved overall growth of plantlets. Also, plantlets raised through prolonged culture were acclimatized with 94% survival efficiency in comparison to 46% with normal culture period, and showed improved growth performance on transfer to soil in the nursery, irrespective of the culture vessel size.

Keywords


Acclimatization, in vitro Hardening, Lab-to-Land Transfer, Prolonged Culture, Tea Micropropagation.

References





DOI: https://doi.org/10.18520/cs%2Fv117%2Fi2%2F288-293