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Soybean Methylation Analysis during Strontium Stress using Methylation-Sensitive Amplified Polymorphism


Affiliations
1 Life Science College of Anhui Science and Technology University, Fengyang, Auhui 233100, China
 

The effect of strontium stress on the pattern and degree of DNA methylation in soybean seedlings was analysed using the methylation-sensitive amplified polymorphism (MSAP) method. The growth traits were inhibited by SrCl2 treatments. A total of 167 loci were determined and evaluated for DNA methylation after different treatments. The level of cytosine methylation initially decreased and then increased with increasing Sr concentration. Methylation was lowest after 10 mmol/l SrCl2 treatment. Strontium stress resulted in a 57.48% alteration of DNA methylation patterns in 5′-CCGG-3′ loci. The pattern variation initially decreased and then increased along with increasing strontium concentration. There was a positive correlation between the total methylation and full methylation induced by strontium stress, and weight and length of shoots and ischolar_mains in soybean. Overall, the changes in the pattern and degree of methylation may be a key regulatory mechanism for soybean adaptation to strontium.

Keywords

Methylation/Demethylation, Polymorphism, Soybean, Strontium Stress.
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  • Kan, U., Koichi, K., Tomotaro, D., Misuzu, W., Hiroyasu, S., Yoshimi, T. and Emi, Y., An overview of boron, lithium, and strontium in human health and profiles of these elements in urine of Japanese. Environ. Health Prev. Med., 2007, 12, 231–237.

  • Xie, H. and Ye, Q., Strontium: friend or foe of bone formation? Osteoporosis Int., 2015, 26, 2213–2214.

  • Cohen-Solal, M., Strontium overload and toxicity: impact on renal osteodystrophy. Nephrol. Dial. Transpl., 2002, 17, 30–34.

  • Sreedhar, B., Satya Vani, Ch., Keerthi Devi, D., Sreeram, V. and Basaveswara Rao, M. V., Nucleation controlled in the aggregative growth of strontium carbonate microcrystals. Am. J. Mater. Sci., 2012, 2(5), 142–146.

  • Chen, M., Tang, Y. L., Ao, J. and Wang, D., Effect of strontium on photosynthetic characteristics of oilseed rape seedlings. Russ. J. Plant Physiol., 2012, 59, 772–780.

  • Kamangar, A. and Haddad, R., Effect of water stress and sodium silicate on antioxidative response in different grapevine (Vitis vinifera L.) cultivars. J. Agric. Sci. Technol., 2016, 18, 1859–1870.

  • Sha, A. H., Lin, X. H., Huang, J. B. and Zhang, D. P., Analysis of DNA methylation related to rice adult plant resistance to bacterial blight based on methylation-sensitive AFLP (MSAP) analysis. Mol. Gen. Genomics, 2005, 273, 484–490.

  • Shan, X. H. et al., Analysis of the DNA methylation of maize (Zea mays L.) in response to cold stress based on methylation-sensitive amplified polymorphisms. J. Plant Biol., 2013, 56, 32–38.

  • Tan, M. P., Analysis of DNA methylation of maize in response to osmotic and salt stress based on methylation-sensitive amplified polymorphism. Plant Physiol. Biochem., 2010, 48, 21–26.

  • Keyte, A. L., Percifield, R., Liu, B. and Wendel, J. F., Infraspecific DNA methylation polymorphism in cotton (Gossypium hirsutum L.). J. Hered., 2006, 97, 444–450.

  • Xin, C. H. et al., Analysis of cytosine methylation status in potato by methylation-sensitive amplified polymorphisms under lowtemperature stress. J. Plant Biol., 2015, 58, 383–390.

  • Bonasio, R., Tu, S. and Reinberg, D., Molecular signals of epigenetic states. Science, 2010, 330, 612–616.

  • Choi, C. S. and Sano, H., Abiotic-stress induces demethylation and transcriptional activation of a gene encoding a glycerophosphodiesteraselike protein in tobacco plants. Mol. Genet. Genomics, 2007, 277, 589–600.

  • Heller, G., Zielinski, C. C. and Zöchbauer-Müller, S., Lung cancer: from single-gene methylation to methylome profiling. Cancer Metastasis Rev., 2010, 29(1), 95–107.

  • Jaligot, E., Beuléand, T. and Rival, A., Methylation-sensitive RFLPs: characterization of two oil palm markers showing somaclonal variation-associated polymorphism. Theor. Appl. Genet., 2002, 104, 1263–1269.

  • Capuano, F., Mulleder, M., Kok, R., Blom, H. J. and Ralser, M., Cytosine DNA methylation is found in Drosophila melanogaster but absent in Saccharomyces cerevisiae, Schizosaccharomyces pombe, and other yeast species. Anal. Chem., 2014, 86, 3697– 3702.

  • Cicatelli, A., Todeschini, V., Lingua, G., Biondi, S., Torrigiani, P. and Castiglione, S., Epigenetic control of heavy metal stress response in mycorrhizal versus non-mycorrhizal poplar plants. Environ. Sci. Pollut. Res. Int., 2014, 21, 1723–1737.

  • Tang, X. M., Tao, X., Wang, Y., Ma, D. W., Li, D., Yang, H. and Ma, X. R., Analysis of DNA methylation of perennial ryegrass under drought using the methylation sensitive amplification polymorphism (MSAP) technique. Mol. Genet. Genomics, 2014, 289, 1075–1084.

  • Cervera, M. T., Ruiz-Garcia, L. and Martinez-Zapater, J., Analysis of DNA methylation in Arabidopsis thaliana based on methylationsensitive AFLP markers. Mol. Genet. Genomics, 2002, 268, 543–552.

  • Lam, H. M. et al., Resequencing of 31 wild and cultivated soybean genomes identifies patterns of genetic diversity and selection. Nature Genet., 2010, 42, 1053–1059.

  • Wójciak-Kosior, M. et al., The stimulatory effect of strontium ions on phytoestrogens content in Glycine max (L.) Merr. Molecules, 2016, 21; doi:10.3390/ molecules21010090.

  • Xiong, D. J., Zhao, T. J. and Gai, J. Y., Parental analysis of soybean cultivars released in China. Sci. Agric. Sin., 2008, 41(9), 2589–2598 (in Chinese).

  • Fehr, W. R., Caviness, C. E., Burmood, D. T. and Pennington, J. S., Stage of development descriptions for soybeans, Glycine max (L.) Merrill. Crop Sci., 1971, 11(6), 929–931.

  • Saghai-Maroof, M. A., Soliman, K. M., Jorgensen, R. A. and Allard, R. W., Ribosomal DNA spacer-length polymorphisms in barley: mendelian inheritance, chromosomal location, and population dynamics. Proc. Natl. Acad. Sci. USA, 1984, 81(24), 8014– 8018.

  • Xiong, L. Z., Xu, C. G., Saghai-Maroof, M. A. and Zhang, Q. F., Patterns of cytosine methylation in an elite rice hybrid and its parental lines by a methylation-sensitive amplification polymorphism technique. Mol. Gen. Genet., 1999, 261, 439–446.

  • Wang, W. S. et al., Drought-induced site-specific DNA methylation and its association with drought tolerance in rice (Oryza sativa L.) J. Exp. Bot., 2011, 62(6), 1951–1960.

  • Jaroslav, F. and Aleš, K., How to interpret methylation sensitive amplified polymorphism (MSAP) profiles? BMC Genetics, 2014; doi:10.1186/1471-2156-15-2.

  • Cao, D. H. et al., Methylation sensitive amplified polymorphism (MSAP) reveals that alkali stress triggers more DNA hypomethylation levels in cotton (Gossypium hirsutum L.) ischolar_mains than salt stress. Afr. J. Biotechnol., 2011, 10, 18971–18980.

  • Zhao, Y. L., Yu, S. X., Ye, W. W., Wang, H. M., Wang, J. J. and Fang, X., Study on DNA cytosine methylation of cotton (Gossypium hirsutum L.) genome and its implication for salt tolerance. Agric. Sci. China, 2010, 9, 783–791.


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  • Soybean Methylation Analysis during Strontium Stress using Methylation-Sensitive Amplified Polymorphism

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Authors

Qingyuan He
Life Science College of Anhui Science and Technology University, Fengyang, Auhui 233100, China
Zhengpeng Li
Life Science College of Anhui Science and Technology University, Fengyang, Auhui 233100, China
Yingjie Shu
Life Science College of Anhui Science and Technology University, Fengyang, Auhui 233100, China
Songhua Wang
Life Science College of Anhui Science and Technology University, Fengyang, Auhui 233100, China
Shoucheng Huang
Life Science College of Anhui Science and Technology University, Fengyang, Auhui 233100, China

Abstract


The effect of strontium stress on the pattern and degree of DNA methylation in soybean seedlings was analysed using the methylation-sensitive amplified polymorphism (MSAP) method. The growth traits were inhibited by SrCl2 treatments. A total of 167 loci were determined and evaluated for DNA methylation after different treatments. The level of cytosine methylation initially decreased and then increased with increasing Sr concentration. Methylation was lowest after 10 mmol/l SrCl2 treatment. Strontium stress resulted in a 57.48% alteration of DNA methylation patterns in 5′-CCGG-3′ loci. The pattern variation initially decreased and then increased along with increasing strontium concentration. There was a positive correlation between the total methylation and full methylation induced by strontium stress, and weight and length of shoots and ischolar_mains in soybean. Overall, the changes in the pattern and degree of methylation may be a key regulatory mechanism for soybean adaptation to strontium.

Keywords


Methylation/Demethylation, Polymorphism, Soybean, Strontium Stress.

References





DOI: https://doi.org/10.18520/cs%2Fv117%2Fi3%2F486-491