Current Science

Open Access Open Access  Restricted Access Subscription Access

Modulation in Activity Profiles in Insecticide-Resistant Population of Tobacco Caterpillar, Spodoptera Litura (Fabricius)


Affiliations
1 Department of Entomology, College of Agriculture, Vellayani, Thiruvananthapuram 695 522, India
2 Department of Plant Pathology, College of Agriculture, Vellayani, Thiruvananthapuram 695 522, India
3 ICAR-Central Plantation Crops Research Institute, Kayankulam 690 533, India
 

Activity spectrum of detoxification enzymes was systematically assessed in tobacco caterpillar, Spodoptera litura collected from four locations in Kerala, India, to decipher the mechanism of insecticide resistance. Using the susceptible check ICAR-NBAIR strain, specific activity profiles of acetylcholine esterase (AChE) were found to be 16.16-, 10.71- and 4.88-fold higher in the Kovilnada, Palappur and Kanjikuzhi populations respectively. Specific activities of mixed function oxidase (MFO) were also found to be 19.24-, 17.11-, 6.08-fold higher in the same populations respectively, indicating the predominance of AChE and MFO towards imparting resistance. Carboxylesterase (CarE) and glutathion-S-transferase (GST) specific activity profiles were 3.62- and 3.37-fold higher in the Kovilnada population, followed by 2.89- and 2.98-fold higher in the Palappur population and as 2.10- and 1.15-fold higher in the Kanjikuzhi population, indicating their partial role in resistance development. Suppression of specific activities in synergism bioassays with AChE in chlorpyriphos + TPP treatment (9.32-fold), GST in chlorpyriphos + DEM (4.78-fold) and CarE in quinalphos + TPP (5.15-fold) highlighted the involvement of multiple detoxification enzymes conferring resistance to organophosphates. Reduced activity of MFO in case of lambda-cyhalothrin + PBO (5.35-fold), CarE in case of cypermethrin + TPP (7.36-fold) and 3.60-fold reduction in MFO in case of cypermethrin + PBO highlighted the role of esterases and MFOs towards resistance development against synthetic pyrethroids.

Keywords

Detoxification Enzymes, Insecticide Resistance, Spodoptera litura, Synergists.
User
Notifications
Font Size

  • Brattsten, L. B., Potential role of plant allelochemicals in the development of insecticide resistance. In Novel Aspects of Insect Plant Interactions (eds Barbosa, P. and Letourneau, D. K.), Wiley, New York, 1986, pp. 313–348.

  • Arthropod pesticide resistance database, 2017; http://www.pesticideresistance.com (accessed on 16 May 2017).

  • Dhir, B. C., Mohapatra, H. K. and Senapati, B., Assessment of crop loss in groundnut due to tobacco caterpillar, Spodoptera litura (F.). Indian J. Plant Prot., 1992, 20, 215–217.

  • Qin, H., Ye, Z., Huang, S., Ding, J. and Luo, R., The correlations of the different host plant with preference level, life duration and survival rate of Spodoptera litura Fabricius. Chin. J. Eco.-Agric., 2004, 12, 40–42. (Original not seen. Cited by Ahmad, M., Sayyed, A. H., Crickmore, N. and Saleem, M. A.). Pest Manage. Sci., 2007, 63, 1002–1010).

  • Abbas, N., Samiullah, Shad, S., Aslam, M., Razaq, M. and Waheed, A., Resistance of Spodoptera litura (Lepidoptera: Noctuidae) to profenofos: relative fitness and cross resistance. Crop Prot., 2014, 58, 49–54.

  • Saleem, M., Hussain, D., Ghouse, G., Abbas, M. and Fisher, S. W., Monitoring of insecticide resistance in Spodoptera litura (Lepidoptera: Noctuidae) from four districts of Punjab, Pakistan to conventional and new chemistry insecticides. Crop Prot., 2016, 79, 177–184.

  • Yu, S. J., Insensitivity of acetylcholinesterase in a field strain of the fall armyworm, Spodoptera frugiperda (J. E. Smith). Pestic. Biochem. Physiol., 2006, 84, 135–142.

  • Huang, S. and Han, Z., Mechanisms for multiple resistance in field populations of common cutworm, Spodoptera litura (Fabricius) in China. Pestic. Biochem. Physiol., 2007, 87, 14–22.

  • Armes, N. J., Wightman, J. A., Jadhav, D. R. and Rao, G. V. R., Status of insecticide resistance in Spodoptera litura in Andhra Pradesh, India. Pestic. Manage. Sci., 1997, 50(3), 240–248.

  • Karuppaiah, V., Srivastava, C. and Subramanian, S., Variation in insecticide detoxification enzymes activity in Spodoptera litura (Fabricius) of different geographic origin. J. Entomol. Zool. Stud., 2017, 5(3), 770–773.

  • Sreelakshmi, P., Mathew, T. B., Josephrajkumar, A. and Paul, A., Synergist induced susceptibility of tobacco caterpillar, Spodoptera litura (Fabricius) from Kerala, India exposed to conventional insecticides. Phytoparasitica, 2018, 46, 97–104.

  • Kranthi, K. R., Insecticide Resistance – Monitoring, Mechanisms and Management Manual, Central Institute for Cotton Research, Nagpur, and ICAC, Washington, 2005, p. 150.

  • Insecticide Resistance Action Committee, 2014; http://www.iraconline.org (accessed on 18 November 2016).

  • Ellman, G. L., Courtney, K. D., Andres, V. and Featherstone, R. M., A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem. Pharmacol., 1961, 7, 88–95.

  • Hansen, L. G. and Hodgson, E., Biochemical characteristics of insect microsomes: N- and O-demethylation. Biochem. Pharmacol., 1971, 20, 1569–1573.

  • Cheema, H. K., Evaluation of insecticide resistance profile in Spodoptera litura (Fabricius) populations through biological, biochemical and molecular diagnosis. Ph D dissertation. Punjab Agricultural University, Ludhiana, 2013, p. 132.

  • Bradford, M. M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 1976, 72, 248–254.

  • Feyereisen, R., Molecular biology of insecticide resistance. Toxicol. Lett., 1993, 82/83, 83–90.

  • Sreelakshmi, P., Mathew, T. B., Muralikrishna, P. and Paul, A., Insecticide resistance in field populations of tobacco caterpillar, Spodoptera litura Fabricius (Lepidoptera: Noctuidae). Entomon, 2017, 42(1), 41–46.

  • Hemingway, J. and Ranson, H., Insecticide resistance in insect vectors of human disease. Annu. Rev. Entomol., 2000, 45(1), 371– 391.

  • Srigiriraju, L., Semtner, P. J., Anderson, T. D. and Bloomquist, J. R., Esterase-based resistance in the tobacco-adapted form of the green peach aphid, Myzus persicae (Sulzer) (Hemiptera: Aphididae) in the eastern United States. Arch. Insect. Biochem. Physiol., 2009, 72(2), 105–123.

  • Kaur, P. and Kang, B. K., Effect of selected synergists on insecticidal toxicity of deltamethrin and biochemical mechanisms on the field populations of tobacco caterpillar from Punjab, India. Phytoparasitica, 2015, 43(4), 565–575.

  • Rodriguez, M. A., Marques, T., Bosch, D. and Avilla, J., Assessment of insecticide resistance in eggs and neonate larvae of Cydia pomonella (Lepidoptera: Tortricidae). Pestic. Biochem. Physiol., 2011, 100, 151–159.

  • Scott, J. G., Cytochromes P450 and insecticide resistance. Insect Biochem. Mol. Biol., 1999, 29, 757–777.

  • Su, J., Lai, T. and Li, J., Susceptibility of field populations of Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae) in China to chlorantraniliprole and the activities of detoxification enzymes. Crop Prot., 2012, 42, 217–222.

  • Yang, Y., Wu, Y., Chen, S., Devine, G. J., Denholm, I., Jewess, P. and Moores, G. D., The involvement of microsomal oxidases in pyrethroid resistance in Helicoverpa armigera from Asia. Insect Biochem. Mol. Biol., 2004, 34, 763–773.

  • Muthusamy, S., Shivakumar, M. S., Karthi, R. and Ramkumar, G., Pesticide detoxifiying mechanism in field population of Spodoptera litura (Lepidoptera: Noctuidae) from South India. Egypt. Acad. J. Biol. Sci., 2011, 3(1), 51–57.

  • Sayyed, A. H., Ahmad, M. and Saleem, M. A., Cross resistance and genetics of resistance to indoxacarb in Spodoptera litura (Lepidoptera: Noctuidae). J. Econ. Entomol., 2008, 101(2), 472– 479.

  • Ahmad, M., Arif, M. I. and Ahmad, M., Occurrence of insecticide resistance in field populations of Spodoptera litura (Lepidoptera: Noctuidae) in Pakistan. Crop Prot., 2007, 26, 809–817.

  • Shad, S. A., Sayyed, A. H. and Saleem, M. A., Cross-resistance, mode of inheritance and stability of resistance to emamectin in Spodoptera litura Fab. (Lepidoptera: Noctuidae). Pest Manage. Sci., 2010, 66, 839–846.


Abstract Views: 314

PDF Views: 129




  • Modulation in Activity Profiles in Insecticide-Resistant Population of Tobacco Caterpillar, Spodoptera Litura (Fabricius)

Abstract Views: 314  |  PDF Views: 129

Authors

P. Sreelakshmi
Department of Entomology, College of Agriculture, Vellayani, Thiruvananthapuram 695 522, India
Thomas Biju Mathew
Department of Entomology, College of Agriculture, Vellayani, Thiruvananthapuram 695 522, India
K. Umamaheswaran
Department of Plant Pathology, College of Agriculture, Vellayani, Thiruvananthapuram 695 522, India
A. Josephrajkumar
ICAR-Central Plantation Crops Research Institute, Kayankulam 690 533, India

Abstract


Activity spectrum of detoxification enzymes was systematically assessed in tobacco caterpillar, Spodoptera litura collected from four locations in Kerala, India, to decipher the mechanism of insecticide resistance. Using the susceptible check ICAR-NBAIR strain, specific activity profiles of acetylcholine esterase (AChE) were found to be 16.16-, 10.71- and 4.88-fold higher in the Kovilnada, Palappur and Kanjikuzhi populations respectively. Specific activities of mixed function oxidase (MFO) were also found to be 19.24-, 17.11-, 6.08-fold higher in the same populations respectively, indicating the predominance of AChE and MFO towards imparting resistance. Carboxylesterase (CarE) and glutathion-S-transferase (GST) specific activity profiles were 3.62- and 3.37-fold higher in the Kovilnada population, followed by 2.89- and 2.98-fold higher in the Palappur population and as 2.10- and 1.15-fold higher in the Kanjikuzhi population, indicating their partial role in resistance development. Suppression of specific activities in synergism bioassays with AChE in chlorpyriphos + TPP treatment (9.32-fold), GST in chlorpyriphos + DEM (4.78-fold) and CarE in quinalphos + TPP (5.15-fold) highlighted the involvement of multiple detoxification enzymes conferring resistance to organophosphates. Reduced activity of MFO in case of lambda-cyhalothrin + PBO (5.35-fold), CarE in case of cypermethrin + TPP (7.36-fold) and 3.60-fold reduction in MFO in case of cypermethrin + PBO highlighted the role of esterases and MFOs towards resistance development against synthetic pyrethroids.

Keywords


Detoxification Enzymes, Insecticide Resistance, Spodoptera litura, Synergists.

References





DOI: https://doi.org/10.18520/cs%2Fv116%2Fi4%2F664-669