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A Practive Faeces Collection Protocol for Multidisciplinary Research in Wildlife Science


Affiliations
1 Wildlife Institute of India, Chandrabani, Dehradun 248 001, India
 

Faecal samples have become an important noninvasive source of information in wildlife biology and ecological research. Despite regular use of faeces, there is no universal protocol available for faeces collection and storage to answer various questions in wildlife biology. In this study we collected 1408 faeces from ten different species using a dry sampling approach, and achieved 77.49% and 75.25% success rate in mitochondrial and nuclear marker amplifications respectively. We suggest a universal framework to use the same samples to answer different questions. This protocol provides an easy, quick and cheap option to collect non-invasive samples from species living in different environmental conditions to answer multidisciplinary questions in wildlife biology.

Keywords

Non-invasive Wildlife Research, Species Biology, Dry Sampling, Variable Habitats, Field Logistics.
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  • A Practive Faeces Collection Protocol for Multidisciplinary Research in Wildlife Science

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Authors

Suvankar Biswas
Wildlife Institute of India, Chandrabani, Dehradun 248 001, India
Supriya Bhatt
Wildlife Institute of India, Chandrabani, Dehradun 248 001, India
Shrutarshi Paul
Wildlife Institute of India, Chandrabani, Dehradun 248 001, India
Shrushti Modi
Wildlife Institute of India, Chandrabani, Dehradun 248 001, India
Tista Ghosh
Wildlife Institute of India, Chandrabani, Dehradun 248 001, India
Bilal Habib
Wildlife Institute of India, Chandrabani, Dehradun 248 001, India
Parag Nigam
Wildlife Institute of India, Chandrabani, Dehradun 248 001, India
Gautam Talukdar
Wildlife Institute of India, Chandrabani, Dehradun 248 001, India
Bivash Pandav
Wildlife Institute of India, Chandrabani, Dehradun 248 001, India
Samrat Mondol
Wildlife Institute of India, Chandrabani, Dehradun 248 001, India

Abstract


Faecal samples have become an important noninvasive source of information in wildlife biology and ecological research. Despite regular use of faeces, there is no universal protocol available for faeces collection and storage to answer various questions in wildlife biology. In this study we collected 1408 faeces from ten different species using a dry sampling approach, and achieved 77.49% and 75.25% success rate in mitochondrial and nuclear marker amplifications respectively. We suggest a universal framework to use the same samples to answer different questions. This protocol provides an easy, quick and cheap option to collect non-invasive samples from species living in different environmental conditions to answer multidisciplinary questions in wildlife biology.

Keywords


Non-invasive Wildlife Research, Species Biology, Dry Sampling, Variable Habitats, Field Logistics.

References





DOI: https://doi.org/10.18520/cs%2Fv116%2Fi11%2F1878-1885