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Transcriptomic Analysis of Chilling-treated Tobacco (Nicotiana tabacum) Leaves Reveals Chilling-Induced Lignin Biosynthetic Pathways


Affiliations
1 Key Laboratory of Tobacco Biology and Processing, Ministry of Agriculture, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, China
2 Longyan Tobacco Agricultural Science Institute, Longyan, Fujian 364000,, China
 

Chilling stress is one of the most important environ-mental stresses for chilling-sensitive species. The pre-sent study conducted RNA-Seq and WGCNA analysis to clarify the correlation patterns among genes of dif-ferent treatments in tobacco (Nicotiana tabacum). A total of 10,355 DEGs were found in chilling treatment relative to control treatment. Additionally, functional annotations revealed that 48 genes were found to be specifically expressed in lignin biosynthesis pathway in tobacco seedlings under chilling stress. Our results revealed that the biosynthesis of caffeoyl-CoA was regulated by HCT and C3H. Furthermore, the G-type lignin biosynthesis branch was enhanced under low temperature, which contributed to an increase in lig-nin content and changes in lignin composition, indi-cating that G-type lignin may play an important role in tobacco’s resistance to chilling stress.

Keywords

Chilling Stress, Lignin Biosynthesis, Nicotiana Tabacum, Transcriptomic, WGCNA.
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Abstract Views: 340

PDF Views: 131




  • Transcriptomic Analysis of Chilling-treated Tobacco (Nicotiana tabacum) Leaves Reveals Chilling-Induced Lignin Biosynthetic Pathways

Abstract Views: 340  |  PDF Views: 131

Authors

PeiLu Zhou
Key Laboratory of Tobacco Biology and Processing, Ministry of Agriculture, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, China
QiYao Li
Key Laboratory of Tobacco Biology and Processing, Ministry of Agriculture, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, China
Guang Liang Liu
Key Laboratory of Tobacco Biology and Processing, Ministry of Agriculture, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, China
Na Xu
Key Laboratory of Tobacco Biology and Processing, Ministry of Agriculture, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, China
Yin Ju Yang
Key Laboratory of Tobacco Biology and Processing, Ministry of Agriculture, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, China
Yi Wang
Key Laboratory of Tobacco Biology and Processing, Ministry of Agriculture, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, China
Wen Long Zeng
Longyan Tobacco Agricultural Science Institute, Longyan, Fujian 364000,, China
Shu Sheng Wang
Key Laboratory of Tobacco Biology and Processing, Ministry of Agriculture, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, China
Ai Guo Chen
Key Laboratory of Tobacco Biology and Processing, Ministry of Agriculture, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, China

Abstract


Chilling stress is one of the most important environ-mental stresses for chilling-sensitive species. The pre-sent study conducted RNA-Seq and WGCNA analysis to clarify the correlation patterns among genes of dif-ferent treatments in tobacco (Nicotiana tabacum). A total of 10,355 DEGs were found in chilling treatment relative to control treatment. Additionally, functional annotations revealed that 48 genes were found to be specifically expressed in lignin biosynthesis pathway in tobacco seedlings under chilling stress. Our results revealed that the biosynthesis of caffeoyl-CoA was regulated by HCT and C3H. Furthermore, the G-type lignin biosynthesis branch was enhanced under low temperature, which contributed to an increase in lig-nin content and changes in lignin composition, indi-cating that G-type lignin may play an important role in tobacco’s resistance to chilling stress.

Keywords


Chilling Stress, Lignin Biosynthesis, Nicotiana Tabacum, Transcriptomic, WGCNA.

References





DOI: https://doi.org/10.18520/cs%2Fv117%2Fi11%2F1885-1892