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Glucose Uptake Rate of Microorganisms Living in Hot Springs above 70°c Temperature:A Study of Panamik and Puga Hot Springs in the Ladakh Region, Jammu and Kashmir, India


Affiliations
1 Birbal Sahni Institute of Palaeosciences, 53 University Road, Lucknow 226 007, India
 

This study measures in situ microbial glucose uptake rate in two different hot springs in Ladakh, J&K, India with distinct temperatures >74°C and pH > 7.4. For this purpose, the slurry samples from each hot spring were incubated up to 4 h with 13C-labelled glucose in gas-tight incubation bottles at the respective hot-spring sites. The natural δ13C particulate varies from –9.1‰ in Panamik hot spring to –11.7‰ in Puga hot spring. After incubation with 13C-labelled glucose, the δ13C particulate reached a maximum 2472‰ in Panamik and 4365‰ in Puga hot-spring samples. The glucose uptake rate calculated from the final δ13C particulate in the incubation bottles varied from 28 to 147 ng C g–1 h–1 in the Panamik and from 168 to 1196 ng C g–1 h–1 in the Puga samples. This reveals that even at >74°C temperature, thermophiles are capable of running their metabolic machinery, perhaps faster than the heterotrophic microbes/cells under normal temperature condition.

Keywords

Exogenous Carbon, Hot Springs, Thermophiles Glucose Uptake.
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  • Allen, C. C. and Oehler, D. Z., A case for ancient springs in Arabia Terra, Mars. Astrobiology, 2008, 8, 1093–1112.

  • Rothschild, L. J. and Mancinelli, R. L., Life in extreme environments. Nature, 2001, 409, 1092.

  • Magan, N., Fungi in extreme environments. Mycota, 2007, 4, 85– 103.

  • Seckbach, J. and Oren, A., Oxygenic photosynthetic microorganisms in extreme environments. In Algae and Cyanobacteria in Extreme Environments, Springer, The Netherlands, 2007, pp. 3– 25.

  • Swingley, W. D., D’Arcy, R., Shock, E. L., Alsop, E. B., Falenski, H. D., Havig, J. R. and Raymond, J., Coordinating environmental genomics and geochemistry reveals metabolic transitions in a hot spring ecosystem. PLoS ONE, 2012, 7, e38108.

  • Schubotz, F., Meyer‐Dombard, D., Bradley, A., Fredricks, H., Hinrichs, K. U., Shock, E. and Summons, R., Spatial and temporal variability of biomarkers and microbial diversity reveal metabolic and community flexibility in streamer biofilm communities in the Lower Geyser Basin, Yellowstone National Park. Geobiology, 2013, 11, 549–569.

  • Schubotz, F., Hays, L. E., Meyer-Dombard, D. A. R., Gillespie, A., Shock, E. L. and Summons, R. E., Stable isotope labeling confirms mixotrophic nature of streamer biofilm communities at alkaline hot springs. Front. Microbiol., 2015, 6, 42.

  • Wang, S., Dong, H., Hou, W., Jiang, H., Huang, Q., Briggs, B. R. and Huang, L., Greater temporal changes of sediment microbial community than its waterborne counterpart in Tengchong hot springs, Yunnan Province, China. Sci. Rep., 2014, 4, 7479.

  • Meyer‐Dombard, D. A. R., Swingley, W., Raymond, J., Havig, J., Shock, E. L. and Summons, R. E., Hydrothermal ecotones and streamer biofilm communities in the Lower Geyser Basin, Yellowstone National Park. Environ. Microbiol., 2011, 13, 2216– 2231.

  • Tiwari, S. K., Rai, S. K., Bartarya, S. K., Gupta, A. K. and Negi, M., Stable isotopes (δ13CDIC, δ D, δ 18O) and geochemical characteristics of geothermal springs of Ladakh and Himachal (India): evidence for CO2 discharge in Northwest Himalaya. Geothermics, 2016, 64, 314–330.

  • Acharya, S. and Chaudhary, A., Effect of nutritional and environmental factors on cellulases activity by thermophilic bacteria isolated from hot spring. J. Sci. Indus. Res., 2011, 70, 142–148.

  • Ghosh, W., Mallick, S., Haldar, P. K., Pal, B., Maikap, S. C. and Gupta, S. K. D., Molecular and cellular fossils of a mat-like microbial community in geothermal boratic sinters. Geomicrobiol. J., 2012, 29, 879–885.

  • Upadhyay, R., Middle Cretaceous carbonate build-ups and volcanic seamount in the Shyok suture, northern Ladakh, India. Curr. Sci., 2001, 81(6), 695–699.

  • Rao, D. R. and Rai, H., Geochemical studies of granitoids from Shyok tectonic zone of Khardung–Panamik section, Ladakh, India. J. Geol. Soc. India, 2009, 73, 553–566.

  • Montoya, J. P., Voss, M., Kahler, P. and Capone, D. G., A simple, high-precision, high-sensitivity tracer assay for N (inf2) fixation. Appl. Environ. Microbiol., 1996, 62, 986–993.

  • Antonio, R. M. and Bianchini Jr I., The effect of temperature on the glucose cycling and oxygen uptake rates in the Infernão Lagoon water, State of São Paulo, Brazil. Acta Sci., 2002, 24, 291–296.

  • Etxeberria, E., González, P., Tomlinson, P. and Pozueta-Romero, J., Existence of two parallel mechanisms for glucose uptake in heterotrophic plant cells. J. Exp. Bot., 2005, 56, 1905–1912.

  • Conde, C., Agasse, A., Glissant, D., Tavares, R., Gerós, H. and Delrot, S., Pathways of glucose regulation of monosaccharide transport in grape cells. Plant Physiol., 2006, 141, 1563–1577.

  • Morales-Sánchez, D., Martinez-Rodriguez, O. A., Kyndt, J. and Martinez, A., Heterotrophic growth of microalgae: metabolic aspects. World J. Microbiol. Biotechnol., 2015, 31, 1–9.


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  • Glucose Uptake Rate of Microorganisms Living in Hot Springs above 70°c Temperature:A Study of Panamik and Puga Hot Springs in the Ladakh Region, Jammu and Kashmir, India

Abstract Views: 229  |  PDF Views: 70

Authors

A. H. Ansari
Birbal Sahni Institute of Palaeosciences, 53 University Road, Lucknow 226 007, India
Mukund Sharma
Birbal Sahni Institute of Palaeosciences, 53 University Road, Lucknow 226 007, India
S. Ahmad
Birbal Sahni Institute of Palaeosciences, 53 University Road, Lucknow 226 007, India
Veeru Kant Singh
Birbal Sahni Institute of Palaeosciences, 53 University Road, Lucknow 226 007, India
S. K. Pandey
Birbal Sahni Institute of Palaeosciences, 53 University Road, Lucknow 226 007, India
Yogesh Kumar
Birbal Sahni Institute of Palaeosciences, 53 University Road, Lucknow 226 007, India

Abstract


This study measures in situ microbial glucose uptake rate in two different hot springs in Ladakh, J&K, India with distinct temperatures >74°C and pH > 7.4. For this purpose, the slurry samples from each hot spring were incubated up to 4 h with 13C-labelled glucose in gas-tight incubation bottles at the respective hot-spring sites. The natural δ13C particulate varies from –9.1‰ in Panamik hot spring to –11.7‰ in Puga hot spring. After incubation with 13C-labelled glucose, the δ13C particulate reached a maximum 2472‰ in Panamik and 4365‰ in Puga hot-spring samples. The glucose uptake rate calculated from the final δ13C particulate in the incubation bottles varied from 28 to 147 ng C g–1 h–1 in the Panamik and from 168 to 1196 ng C g–1 h–1 in the Puga samples. This reveals that even at >74°C temperature, thermophiles are capable of running their metabolic machinery, perhaps faster than the heterotrophic microbes/cells under normal temperature condition.

Keywords


Exogenous Carbon, Hot Springs, Thermophiles Glucose Uptake.

References





DOI: https://doi.org/10.18520/cs%2Fv118%2Fi4%2F644-648