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A Method for the Identification and Evaluation of Stay-Green Wheat Variety
Leaf senescence is synchronized with grain-filling in wheat. Delayed leaf senescence, or stay-green, can im-prove wheat yield. In this study, a method has been proposed to identify and evaluate the stay-green wheat. First, chlorophyll content of flag leaf was measured by SPAD-502 meter at three-day intervals from seven days after anthesis to physiological matur-ity. Meanwhile, green leaf area duration was visually recorded using 0–9 scale. Secondly, a mathematical model was simulated according to the above pheno-typic dates. Thirdly, the senescence characteristic parameters were calculated, including the time from anthesis to (i) senescence start (Ts ), (ii) complete senescence (To), and (iii) maximum rate of senescence (TMRS), as well as the maximum rate of senescence. Finally, wheat varieties were divided into three stay-green types by hierarchical cluster analysis, including stay-green, early senescence and intermediate type. Based on this approach, a functional stay-green variety, Tainong 18 was screened. Tainong 18 with a delayed Ts and normal rate of senescence (RS) was similar to dSnR of type A under normal irrigation. However, it had earlier Ts and very small RS, and was similar to type B under drought stress. It can be used as a par-ent of crossing combinations in future wheat breeding, due to a longer photosynthetic activity and high yield potential.
Keywords
Cluster Analysis, Leaf Senescence, Staygreen Variety, Wheat.
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