Nanoformulated CPMSN Biomaterial Regulates Proinflammatory Cytokines to Heal Wounds and Kills Drug-Resistant Bacteria

Chitosan (CS) is one of the most abundant biopolymers present on the wings of arthropod members like insects, prawns, etc. It has potential biomedical value in developing drugs and drug delivery systems. Such a biomedically valuable polymer was combined with mesoporous silica nanoparticles (MSNs) to form chitosan-poly (acrylic acid) coated mesoporous silica nanoparicle (CPMSN) material along with poly acrylic acid (PAA) as co-polymer. The selected polymers perfectly interact with the drugs namely topotecan and quercertin after decorating the materials with arginine-glycine-aspartic (RGD) peptide. The formulated CPMSN biomaterial was analysed biologically and chemically for its stability, biocompatibility and sustained release of drugs to heal wounds. In the present study, the efficacy of the formulated biomaterial has been well proven by in vitro and in vivo models. The present finding suggests that the drug-loaded CPMSN biomaterial significantly induces re-epithelialization process by regulating immune cells at the wound sites. The Western blot analysis revealed the activation of proinflammatory genes like NF-kB, TNF, IL-1, MMP-1, MMP-2 and COX2that accounts for enhanced wound-healing cascade activation. The present study also observed antibacterial activity of the formulated biomaterial against selected bacterial species. Thus we can conclude that the CPMSN biomaterial not only possesses wound-healing properties, but also behaves as an antibacterial agent.