Current Science

Open Access Open Access  Restricted Access Subscription Access

Production and Characterization of Antimicrobial Peptides from Bacillus subtilis Isolated from Deep-Sea Core Samples


Affiliations
1 Marine Biotechnology Division, National Institute of Ocean Technology, Ministry of Earth Sciences, Chennai 600 100, India
 

A new strain of Bacillus subtilis isolated from deep-sea core sediment sample (1400 m depth) produced antimicrobial peptides (AMPs) when cultured at 50 and 100 bar pressure conditions. The minimum inhibitory concentrations (MIC) showed that the AMPs had potent activity against V. cholerae and K. pneumoniae. AMPs extracted from cells grown at ambient and elevated pressure conditions exhibited distinct antifungal and antibacterial activities. Analysis of genes encoding AMPs revealed the presence of srfAA, sbo and bmyB biosynthetic genes. GC–MS analysis confirmed substantial accumulation of unsaturated fatty acids in membrane lipids of the cells in response to elevated pressure.

Keywords

Antimicrobial Peptides, Bacillus subtilis, Biosynthetic Genes, Deep-Sea Bacteria, Piezotolerance.
User
Notifications
Font Size

  • Caulier, S., Nannan, C., Gillis, A., Licciardi, F., Bragard, C. and Mahillon, J., Overview of the antimicrobial compounds produced by members of the Bacillus subtilis group. Front. Microbiol., 2019, 302, 1–19.

  • Mondol, M. A., Shin, H. J. and Islam, M. T., Diversity of secondary metabolites from marine Bacillus species: chemistry and biological activity. Mar. Drugs, 2013, 11, 2846–2872.

  • Tareq, F. S., Lee, M. A., Lee, H. S., Lee, J. S., Lee, Y. J. and Shin, H. J., Gageostatins A-C, antimicrobial linear lipopeptides from a marine Bacillus subtilis. Mar. Drugs, 2014, 12, 871–885.

  • Bartlett, D. H., Pressure effects on in vivo microbial processes. Biochim. Biophys. Acta, 2002, 1595, 367–381.

  • Fang, J., Kato, C., Runko, G. M., Nogi, Y., Hori, T., Li, J., Morono, Y. and Inagaki, F., Predominance of viable spore-forming piezophilic bacteria in high-pressure enrichment cultures from ~1.5 to 2.4 km-deep coal-bearing sediments below the Ocean floor. Front. Microbiol., 2017, 8, 1–10.

  • Flores, G. E. et al., Microbial community structure of hydrothermal deposits from geochemically different vent fields along the Mid-Atlantic Ridge. Environ. Microbiol., 2011, 13, 2158–2171.

  • Goffredi, S. K. and Orphan, V. J., Bacterial community shifts in taxa and diversity in response to localized organic loading in the deep sea. Environ. Microbiol., 2010, 12, 344–363.

  • Dang, H., Luan, X. W., Chen, R., Zhang, X., Guo, L. and Klotz, M. G., Diversity, abundance and distribution of amoA-encoding archaea in deep-sea methane seep sediments of the Okhotsk Sea. FEMS Microbiol. Ecol., 2010, 72, 370–385.

  • Dalmaso, G. Z., Ferreira, D. and Vermelho, A. B., Marine extremophiles: a source of hydrolases for biotechnological applications. Mar. Drugs, 2015, 13, 1925–1965.

  • Cristopher, A. B., Liuris, H., Hector, M. G. and Marcelino, G., Antiplasmodial activity of bacilosarcin A isolated from the octocoralassociated bacterium Bacillus sp. collected in Panama. J. Pharm. Bioall. Sci., 2012, 4, 66–69.

  • Hua, N. P., Kanekiyo, A., Fujikura, K., Yasuda, H. and Naganuma, T., Halobacillus profundi sp. nov. and Halobacillus kuroshimensis sp. nov., moderately halophilic bacteria isolated from a deep-sea methane cold seep. Int. J. Syst. Evol. Microbiol., 2007, 57, 1243–1249.

  • Lin, W., Chen, H., Chen, Q., Liu, Y., Jiao, N. and Zheng, Q., Genome sequence of Bacillus sp. CHD6a, isolated from the shallowsea hydrothermal vent. Mar. Genomics, 2016, 25, 15–16.

  • Dick, G. J., Lee, Y. E. and Tebo, B. M., Manganese(II)-oxidizing Bacillus spores in Guaymas basin hydrothermal sediments and plumes. Appl. Environ. Microbiol., 2006, 72, 3184–3190.

  • Bode, H. B., Bethe, B., Hofs, R. and Zeeck, A., Big effects from small changes: possible ways to explore nature’s chemical diversity. Chembiochemistry, 2002, 3, 619–627.

  • Bongers, R. S., Veening, J. W., Van Wieringen, M., Kuipers, O. P. and Kleerebezem, M., Development and characterization of a subtilinregulated expression system in Bacillus subtilis: Strict control of gene expression by addition of subtilin. Appl. Environ. Microbiol., 2005, 71, 8818–8824.

  • Mora, I., Cabrefiga, J. and Montesinos, E., Antimicrobial peptide genes in Bacillus strains from plant environments. Int. Microbiol., 2011, 14, 213–223.

  • Prieto, M. L. et al., Assessment of the bacteriocinogenic potential of marine bacteria reveals lichenicidin production by seaweedderived Bacillus spp. Mar. Drugs, 2012, 10, 2280–2299.

  • Sharma, G., Dang, S., Gupta, S. and Gabrani, R., Antibacterial activity, cytotoxicity, and the mechanism of action of bacteriocin from Bacillus subtilis GAS101. Med. Princ. Pract., 2018, 27, 186–192.

  • Usui, K., Hiraki, T., Kawamoto, J., Kurihara, T., Nogi, Y., Kato, C. and Abe, F., Eicosapentaenoic acid plays a role in stabilizing dynamic membrane structure in the deep-sea piezophile Shewanella violacea: a study employing high-pressure time-resolved fluorescence anisotropy measurement. Biochim. Biophys. Acta, 2012, 1818, 574–583.

  • Vila, T., Romo, J. A., Pierce, C. G., McHardy, S. F., Saville, S. P. and Lopez-Ribot, J. L., Targeting Candida albicans filamentation for antifungal drug development. Virulence, 2017, 8, 150–158.

  • Costa, B. O. and Nahas, E., Growth and enzymatic responses of phytopathogenic fungi to glucose in culture media and soil. Braz. J. Microbiol., 2012, 43, 332–340.

  • Harwood, C. R., Mouillon, J. M., Pohl, S. and Arnau, J., Secondary metabolite production and the safety of industrially important members of the Bacillus subtilis group. FEMS Microbiol. Rev., 2018, 42, 721–728.

  • Kumar, P. and Libchaber, A., Pressure and temperature dependence of growth and morphology of Escherichia coli: experiments and stochastic model. Biophys. J., 2013, 105, 783–793.

  • De Carvalho, C. C. C. R. and Caramujo, M. J., Lipids of prokaryotic origin at the base of marine food webs. Mar. Drugs, 2012, 10, 2698–2714.


Abstract Views: 237

PDF Views: 83




  • Production and Characterization of Antimicrobial Peptides from Bacillus subtilis Isolated from Deep-Sea Core Samples

Abstract Views: 237  |  PDF Views: 83

Authors

A. Ganesh Kumar
Marine Biotechnology Division, National Institute of Ocean Technology, Ministry of Earth Sciences, Chennai 600 100, India
G. Dharani
Marine Biotechnology Division, National Institute of Ocean Technology, Ministry of Earth Sciences, Chennai 600 100, India
R. Kirubagaran
Marine Biotechnology Division, National Institute of Ocean Technology, Ministry of Earth Sciences, Chennai 600 100, India
M. A. Atmanand
Marine Biotechnology Division, National Institute of Ocean Technology, Ministry of Earth Sciences, Chennai 600 100, India

Abstract


A new strain of Bacillus subtilis isolated from deep-sea core sediment sample (1400 m depth) produced antimicrobial peptides (AMPs) when cultured at 50 and 100 bar pressure conditions. The minimum inhibitory concentrations (MIC) showed that the AMPs had potent activity against V. cholerae and K. pneumoniae. AMPs extracted from cells grown at ambient and elevated pressure conditions exhibited distinct antifungal and antibacterial activities. Analysis of genes encoding AMPs revealed the presence of srfAA, sbo and bmyB biosynthetic genes. GC–MS analysis confirmed substantial accumulation of unsaturated fatty acids in membrane lipids of the cells in response to elevated pressure.

Keywords


Antimicrobial Peptides, Bacillus subtilis, Biosynthetic Genes, Deep-Sea Bacteria, Piezotolerance.

References





DOI: https://doi.org/10.18520/cs%2Fv118%2Fi11%2F1725-1730