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Production and Characterization of Antimicrobial Peptides from Bacillus subtilis Isolated from Deep-Sea Core Samples


Affiliations
1 Marine Biotechnology Division, National Institute of Ocean Technology, Ministry of Earth Sciences, Chennai 600 100, India
 

A new strain of Bacillus subtilis isolated from deep-sea core sediment sample (1400 m depth) produced antimicrobial peptides (AMPs) when cultured at 50 and 100 bar pressure conditions. The minimum inhibitory concentrations (MIC) showed that the AMPs had potent activity against V. cholerae and K. pneumoniae. AMPs extracted from cells grown at ambient and elevated pressure conditions exhibited distinct antifungal and antibacterial activities. Analysis of genes encoding AMPs revealed the presence of srfAA, sbo and bmyB biosynthetic genes. GC–MS analysis confirmed substantial accumulation of unsaturated fatty acids in membrane lipids of the cells in response to elevated pressure.

Keywords

Antimicrobial Peptides, Bacillus subtilis, Biosynthetic Genes, Deep-Sea Bacteria, Piezotolerance.
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  • Production and Characterization of Antimicrobial Peptides from Bacillus subtilis Isolated from Deep-Sea Core Samples

Abstract Views: 394  |  PDF Views: 124

Authors

A. Ganesh Kumar
Marine Biotechnology Division, National Institute of Ocean Technology, Ministry of Earth Sciences, Chennai 600 100, India
G. Dharani
Marine Biotechnology Division, National Institute of Ocean Technology, Ministry of Earth Sciences, Chennai 600 100, India
R. Kirubagaran
Marine Biotechnology Division, National Institute of Ocean Technology, Ministry of Earth Sciences, Chennai 600 100, India
M. A. Atmanand
Marine Biotechnology Division, National Institute of Ocean Technology, Ministry of Earth Sciences, Chennai 600 100, India

Abstract


A new strain of Bacillus subtilis isolated from deep-sea core sediment sample (1400 m depth) produced antimicrobial peptides (AMPs) when cultured at 50 and 100 bar pressure conditions. The minimum inhibitory concentrations (MIC) showed that the AMPs had potent activity against V. cholerae and K. pneumoniae. AMPs extracted from cells grown at ambient and elevated pressure conditions exhibited distinct antifungal and antibacterial activities. Analysis of genes encoding AMPs revealed the presence of srfAA, sbo and bmyB biosynthetic genes. GC–MS analysis confirmed substantial accumulation of unsaturated fatty acids in membrane lipids of the cells in response to elevated pressure.

Keywords


Antimicrobial Peptides, Bacillus subtilis, Biosynthetic Genes, Deep-Sea Bacteria, Piezotolerance.

References





DOI: https://doi.org/10.18520/cs%2Fv118%2Fi11%2F1725-1730