Current Science

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Amplicon sequencing of extreme locations helps reveal the presence and abundance of diverse extremophiles within extreme environments. Firmicutes, Proteobacteria and Actinobacteria were identified as a major phyla using 16S rDNA amplicons from the soil and water samples of Tuva Timba and water samples of Dholera in Gujarat, India. Season-wise data when compared with location-wise data showed less diversity. The presence of thermophiles and sulphate-reducing taxa reveals a strong correlation of taxonomy with temperature and sulphate content of the samples. The clear cluster analysis within three sample groups reveals the importance of geographic as well as environmental factors for diverse bacterial community colonization.

Keywords

Amplicon Sequencing, Bacterial Community Analysis, Hot Springs, Metagenomics.
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