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Isolation and Characterization of Indigenous Nucleopolyhedrovirus Infecting Fall Armyworm, Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) in India


Affiliations
1 ICAR-National Bureau of Agricultural Insect Resources, Hebbal, Bengaluru 560 024, India
2 Department of Plant Protection, Horticultural College and Research Institute, Periyakulam, Tamil Nadu Agricultural University, Coimbatore 641 003, India
3 Agricultural Research Station, Borwat Farm, MPUAT, Banswara 327 001, India
 

Fall armyworm (FAW), Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) is an invasive insect pest of maize in India. Natural occurrence of nucleopolyhedrovirus (NPV) infection on S. frugiperda larvae was recorded in 2018 during surveys conducted in maize fields in Chikkaballapura district of Karnataka, and Coimbatore and Tirupattur districts of Tamil Nadu. A strain of S. frugiperda nucleopolyhedrovirus (SpfrNPV NBAIR1) infecting S. frugiperda was isolated from the diseased larvae; morphological and biological characteristics were studied. Electron microscopic studies showed tetrahedral-shaped SpfrNPV occlusion bodies (OBs) of size 1.64 μm. Dose–mortality bioassays revealed that first, second and third instar larvae were equally susceptible (LC50 3.71–5.02 OBs/mm2) to SpfrNPV infection. A PCR technique for detection of viral DNA in S. frugiperda NPV was developed by employing the polyhedrin gene (polh)-specific primers. The amplicon of 618 bp was amplified, sequenced and NCBI GenBank accession number was obtained (MT422725). Blast analysis revealed that SpfrNPV conserved polh gene sequence matched 100% with the reference GenBank sequence (J04333) from the NCBI database which confirmed the identity of the SpfrNPV.

Keywords

Insect Pests, Maize, Nucleopolyhedrovirus, Spodoptera frugiperda.
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  • Isolation and Characterization of Indigenous Nucleopolyhedrovirus Infecting Fall Armyworm, Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) in India

Abstract Views: 343  |  PDF Views: 127

Authors

G. Sivakumar
ICAR-National Bureau of Agricultural Insect Resources, Hebbal, Bengaluru 560 024, India
M. Kannan
Department of Plant Protection, Horticultural College and Research Institute, Periyakulam, Tamil Nadu Agricultural University, Coimbatore 641 003, India
S. Ramesh Babu
Agricultural Research Station, Borwat Farm, MPUAT, Banswara 327 001, India
M. Mohan
ICAR-National Bureau of Agricultural Insect Resources, Hebbal, Bengaluru 560 024, India
M. Sampath Kumar
ICAR-National Bureau of Agricultural Insect Resources, Hebbal, Bengaluru 560 024, India
P. Raveendran
ICAR-National Bureau of Agricultural Insect Resources, Hebbal, Bengaluru 560 024, India
T. Venkatesan
ICAR-National Bureau of Agricultural Insect Resources, Hebbal, Bengaluru 560 024, India
R. Rangeshwaran
ICAR-National Bureau of Agricultural Insect Resources, Hebbal, Bengaluru 560 024, India
Chandish R. Ballal
ICAR-National Bureau of Agricultural Insect Resources, Hebbal, Bengaluru 560 024, India
P. Ram Kumar
ICAR-National Bureau of Agricultural Insect Resources, Hebbal, Bengaluru 560 024, India

Abstract


Fall armyworm (FAW), Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) is an invasive insect pest of maize in India. Natural occurrence of nucleopolyhedrovirus (NPV) infection on S. frugiperda larvae was recorded in 2018 during surveys conducted in maize fields in Chikkaballapura district of Karnataka, and Coimbatore and Tirupattur districts of Tamil Nadu. A strain of S. frugiperda nucleopolyhedrovirus (SpfrNPV NBAIR1) infecting S. frugiperda was isolated from the diseased larvae; morphological and biological characteristics were studied. Electron microscopic studies showed tetrahedral-shaped SpfrNPV occlusion bodies (OBs) of size 1.64 μm. Dose–mortality bioassays revealed that first, second and third instar larvae were equally susceptible (LC50 3.71–5.02 OBs/mm2) to SpfrNPV infection. A PCR technique for detection of viral DNA in S. frugiperda NPV was developed by employing the polyhedrin gene (polh)-specific primers. The amplicon of 618 bp was amplified, sequenced and NCBI GenBank accession number was obtained (MT422725). Blast analysis revealed that SpfrNPV conserved polh gene sequence matched 100% with the reference GenBank sequence (J04333) from the NCBI database which confirmed the identity of the SpfrNPV.

Keywords


Insect Pests, Maize, Nucleopolyhedrovirus, Spodoptera frugiperda.



DOI: https://doi.org/10.18520/cs%2Fv119%2Fi5%2F860-864