Current Science

Open Access Open Access  Restricted Access Subscription Access

Spermatogonial Stem-Cell Transplantation: An Alternative Approach For Transgenesis in Farm Animals


Affiliations
1 Theriogenology Laboratory, Veterinary Gynaecology and Obstetrics, Southern Regional Station of ICAR National Dairy Research Institute, Bengaluru 560 030, India
2 ICAR-National Dairy Research Institute, Karnal 132 001, India
 

Genetic modification of spermatogonial stem cells followed by their transplantation to homologous recipients has shown immense potential to complement the current methods being used for generating transgenic farm animals. Using this technique, the time duration required to generate transgenic founder animals can be reduced at least by half, which would significantly reduce the generation interval. However, significant efforts need to be made to improve the efficiency of transgenic offspring production for its successful implementation in large-scale production of therapeutic proteins for human use.


User
Notifications
Font Size

  • Mehta, P. et al., Agric. Rev., 2017, 38, 129–136.

  • Sharma, A. et al., Cell. Reprogram., 2019, 21, 1–10.

  • Sharma, A. et al., Theriogenology, 2020, 142, 441–449.

  • Honaramooz, A. and Yanfei, Y., Vet. Med. Int., 2011, 2011, 657860.

  • Zeng, W. et al., Biol. Reprod., 2013, 88(1), 27-1.

  • Izadyar, F. et al., Reproduction, 2003, 126, 765–774.

  • Herrid, M. et al., Reprod. Fertil. Dev., 2019, 31(3), 538.

  • Honaramooz, A. et al., Biol. Reprod., 2003, 69, 1260–1264.


Abstract Views: 383

PDF Views: 140




  • Spermatogonial Stem-Cell Transplantation: An Alternative Approach For Transgenesis in Farm Animals

Abstract Views: 383  |  PDF Views: 140

Authors

Ankur Sharma
Theriogenology Laboratory, Veterinary Gynaecology and Obstetrics, Southern Regional Station of ICAR National Dairy Research Institute, Bengaluru 560 030, India
A. Kumaresan
Theriogenology Laboratory, Veterinary Gynaecology and Obstetrics, Southern Regional Station of ICAR National Dairy Research Institute, Bengaluru 560 030, India
M. S. Chauhan
ICAR-National Dairy Research Institute, Karnal 132 001, India

Abstract


Genetic modification of spermatogonial stem cells followed by their transplantation to homologous recipients has shown immense potential to complement the current methods being used for generating transgenic farm animals. Using this technique, the time duration required to generate transgenic founder animals can be reduced at least by half, which would significantly reduce the generation interval. However, significant efforts need to be made to improve the efficiency of transgenic offspring production for its successful implementation in large-scale production of therapeutic proteins for human use.


References





DOI: https://doi.org/10.18520/cs%2Fv120%2Fi2%2F260-261