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16s rRNA and Hydrazine Gene-Based Profiling of the Candidatus Scalindua Community from the Arabian Sea Hypoxic Sediments


Affiliations
1 Biological Oceanography Division, CSIR-National Institute of Oceanography, Goa 403 004, India
2 Academy of Scientific and Innovative Research, Ghaziabad 201 002, India
 

Anammox bacterial diversity and abundance were studied from the organic-rich hypoxic sediments of the Arabian Sea utilizing the partial 16S rRNA, and hydrazine synthase, hzsA and hydrazine oxidoreductase, hzo genes. Among all the clades obtained, phylotypic diversity was high within the Candidatus genus Scalindua with an abundance of ≤7 × 104 copies/g dry wt. As such, Scalindua is known to play a significant role in fixed nitrogen removal through anaerobic ammonium oxidation (anammox) pathway. From these analyses, it is inferred that searching for hzo gene yields robust evidence for detecting anammox community than the widely used 16S rRNA gene marker.

Keywords

Anammox, Community Gene-Based Profiling, Hydrazine, Hypoxic Sediments, Scalindua.
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  • 16s rRNA and Hydrazine Gene-Based Profiling of the Candidatus Scalindua Community from the Arabian Sea Hypoxic Sediments

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Authors

Jovitha Lincy
Biological Oceanography Division, CSIR-National Institute of Oceanography, Goa 403 004, India
Cathrine Sumathi Manohar
Academy of Scientific and Innovative Research, Ghaziabad 201 002, India

Abstract


Anammox bacterial diversity and abundance were studied from the organic-rich hypoxic sediments of the Arabian Sea utilizing the partial 16S rRNA, and hydrazine synthase, hzsA and hydrazine oxidoreductase, hzo genes. Among all the clades obtained, phylotypic diversity was high within the Candidatus genus Scalindua with an abundance of ≤7 × 104 copies/g dry wt. As such, Scalindua is known to play a significant role in fixed nitrogen removal through anaerobic ammonium oxidation (anammox) pathway. From these analyses, it is inferred that searching for hzo gene yields robust evidence for detecting anammox community than the widely used 16S rRNA gene marker.

Keywords


Anammox, Community Gene-Based Profiling, Hydrazine, Hypoxic Sediments, Scalindua.

References





DOI: https://doi.org/10.18520/cs%2Fv120%2Fi4%2F684-693