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Bdnf Protects Photoreceptors from Light-Induced Oxidative Stress Through Upregulating CERKL in vitro


Affiliations
1 Department of Anesthesiology, Zhongnan Hospital of Wuhan University, Wuhan 430071, China
2 Department of Operations Management, Zhongnan Hospital of Wuhan University, Wuhan 430071, China
3 Department of Statistics, Renmin Hospital of Wuhan University, Wuhan 430071, China
4 Department of Orthopedic Surgery, Zhongnan Hospital of Wuhan University, Wuhan 430071, China
 

Brain-derived neurotrophic factor (BDNF) protects the retina from light-induced injury. In this study, white light suppressed the cell viability of 661W photoreceptor cells in a time- and intensity-dependent manner, decreased the mRNA and protein levels of ceramide kinase-like (CERKL), but increased the reactive oxygen species (ROS) content in 24 h. Decrease in ROS content and increase in CERKL level were detected after BDNF treatment. BDNF also attenuated the light-induced ROS accumulation, as well as cell viability and CERKL suppression. Thus, strong light causes CERKL suppression, ROS accumulation and the consequent attenuation of photoreceptor viability, while BDNF protects it from such injuries through upregulating CERKL expression and downregulating ROS accumulation.

Keywords

Brain-derived Neurotrophic Factor, Ceramide Kinase-like, Light-induced Injury, Photoreceptors, Reactive Oxygen Species.
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  • Bdnf Protects Photoreceptors from Light-Induced Oxidative Stress Through Upregulating CERKL in vitro

Abstract Views: 461  |  PDF Views: 122

Authors

Qianwen He
Department of Anesthesiology, Zhongnan Hospital of Wuhan University, Wuhan 430071, China
Juan Yang
Department of Operations Management, Zhongnan Hospital of Wuhan University, Wuhan 430071, China
Mengting Gao
Department of Statistics, Renmin Hospital of Wuhan University, Wuhan 430071, China
Yinxian Wen
Department of Orthopedic Surgery, Zhongnan Hospital of Wuhan University, Wuhan 430071, China

Abstract


Brain-derived neurotrophic factor (BDNF) protects the retina from light-induced injury. In this study, white light suppressed the cell viability of 661W photoreceptor cells in a time- and intensity-dependent manner, decreased the mRNA and protein levels of ceramide kinase-like (CERKL), but increased the reactive oxygen species (ROS) content in 24 h. Decrease in ROS content and increase in CERKL level were detected after BDNF treatment. BDNF also attenuated the light-induced ROS accumulation, as well as cell viability and CERKL suppression. Thus, strong light causes CERKL suppression, ROS accumulation and the consequent attenuation of photoreceptor viability, while BDNF protects it from such injuries through upregulating CERKL expression and downregulating ROS accumulation.

Keywords


Brain-derived Neurotrophic Factor, Ceramide Kinase-like, Light-induced Injury, Photoreceptors, Reactive Oxygen Species.

References





DOI: https://doi.org/10.18520/cs%2Fv120%2Fi6%2F1088-1091