Current Science

Open Access Open Access  Restricted Access Subscription Access

Bdnf Protects Photoreceptors from Light-Induced Oxidative Stress Through Upregulating CERKL in vitro


Affiliations
1 Department of Anesthesiology, Zhongnan Hospital of Wuhan University, Wuhan 430071, China
2 Department of Operations Management, Zhongnan Hospital of Wuhan University, Wuhan 430071, China
3 Department of Statistics, Renmin Hospital of Wuhan University, Wuhan 430071, China
4 Department of Orthopedic Surgery, Zhongnan Hospital of Wuhan University, Wuhan 430071, China
 

Brain-derived neurotrophic factor (BDNF) protects the retina from light-induced injury. In this study, white light suppressed the cell viability of 661W photoreceptor cells in a time- and intensity-dependent manner, decreased the mRNA and protein levels of ceramide kinase-like (CERKL), but increased the reactive oxygen species (ROS) content in 24 h. Decrease in ROS content and increase in CERKL level were detected after BDNF treatment. BDNF also attenuated the light-induced ROS accumulation, as well as cell viability and CERKL suppression. Thus, strong light causes CERKL suppression, ROS accumulation and the consequent attenuation of photoreceptor viability, while BDNF protects it from such injuries through upregulating CERKL expression and downregulating ROS accumulation.

Keywords

Brain-derived Neurotrophic Factor, Ceramide Kinase-like, Light-induced Injury, Photoreceptors, Reactive Oxygen Species.
User
Notifications
Font Size

  • Wert, K. J., Lin, J. H. and Tsang, S. H., General pathophysiology in retinal degeneration. Dev. Ophthalmol., 2014, 53, 33–43.

  • Nakanishi-Ueda, T. et al., Blue LED light exposure develops intracellular reactive oxygen species, lipid peroxidation, and subsequent cellular injuries in cultured bovine retinal pigment epithelial cells. Free Radic. Res., 2013, 47, 774–780.

  • Niwa, M., Aoki, H., Hirata, A., Tomita, H., Green, P. G. and Hara, A., Retinal cell degeneration in animal models. Int. J. Mol. Sci., 2016, 17(1).

  • Grewal, R., Organisciak, D. and Wong, P., Factors underlying circadian dependent susceptibility to light induced retinal damage. Adv. Exp. Med. Biol., 2006, 572, 411–416.

  • Wenzel, A., Grimm, C., Samardzija, M. and Remé, C. E., Molecular mechanisms of light-induced photoreceptor apoptosis and neuroprotection for retinal degeneration. Prog. Retin. Eye Res., 2005, 24, 275–306.

  • Vekslin, S. and Ben-Yosef, T., Spatiotemporal expression pattern of ceramide kinase-like in the mouse retina. Mol. Vis., 2010, 16, 2539–2549.

  • Bornancin, F. et al., Characterization of a ceramide kinase-like protein. Biochim. Biophys. Acta, 2005, 1687, 31–43.

  • Li, C. et al., CERKL interacts with mitochondrial TRX2 and protects retinal cells from oxidative stress-induced apoptosis. Biochim. Biophys. Acta, 2014, 1842, 1121–1129.

  • Cerri, E. et al., Conjunctivally applied BDNF protects photoreceptors from light-induced damage. Transl. Vis. Sci. Technol., 2015, 4, 1.

  • Aleman, T. S. et al., CERKL mutations cause an autosomal recessive cone–rod dystrophy with inner retinopathy. Invest. Ophthalmol. Vis. Sci., 2009, 50, 5944–5954.

  • Harada, T. et al., Modification of glial–neuronal cell interactions prevents photoreceptor apoptosis during light-induced retinal degeneration. Neuron, 2000, 26, 533–541.

  • Harada, T. et al., Microglia–Müller glia cell interactions control neurotrophic factor production during light-induced retinal degeneration. J. Neurosci., 2002, 22, 9228–9236.

  • Kano, T., Abe, T., Tomita, H., Sakata, T., Ishiguro, S. and Tamai, M., Protective effect against ischemia and light damage of iris pigment epithelial cells transfected with the BDNF gene. Invest. Ophthalmol. Vis. Sci., 2002, 43, 3744–3753.

  • King, A., Gottlieb, E., Brooks, D. G., Murphy, M. P. and Dunaief, J. L., Mitochondria-derived reactive oxygen species mediate blue light-induced death of retinal pigment epithelial cells. Photochem. Photobiol., 2004, 79, 470–475.

  • Abed, E., Corbo, G. and Falsini, B., Neurotrophin family members as neuroprotectants in retinal degenerations. BioDrugs, 2015, 29, 1–13.

  • Gabaizadeh, R., Staecker, H., Liu, W. and Van De Water, T. R., BDNF protection of auditory neurons from cisplatin involves changes in intracellular levels of both reactive oxygen species and glutathione. Mol. Brain Res., 1997, 50, 71–78.


Abstract Views: 475

PDF Views: 133




  • Bdnf Protects Photoreceptors from Light-Induced Oxidative Stress Through Upregulating CERKL in vitro

Abstract Views: 475  |  PDF Views: 133

Authors

Qianwen He
Department of Anesthesiology, Zhongnan Hospital of Wuhan University, Wuhan 430071, China
Juan Yang
Department of Operations Management, Zhongnan Hospital of Wuhan University, Wuhan 430071, China
Mengting Gao
Department of Statistics, Renmin Hospital of Wuhan University, Wuhan 430071, China
Yinxian Wen
Department of Orthopedic Surgery, Zhongnan Hospital of Wuhan University, Wuhan 430071, China

Abstract


Brain-derived neurotrophic factor (BDNF) protects the retina from light-induced injury. In this study, white light suppressed the cell viability of 661W photoreceptor cells in a time- and intensity-dependent manner, decreased the mRNA and protein levels of ceramide kinase-like (CERKL), but increased the reactive oxygen species (ROS) content in 24 h. Decrease in ROS content and increase in CERKL level were detected after BDNF treatment. BDNF also attenuated the light-induced ROS accumulation, as well as cell viability and CERKL suppression. Thus, strong light causes CERKL suppression, ROS accumulation and the consequent attenuation of photoreceptor viability, while BDNF protects it from such injuries through upregulating CERKL expression and downregulating ROS accumulation.

Keywords


Brain-derived Neurotrophic Factor, Ceramide Kinase-like, Light-induced Injury, Photoreceptors, Reactive Oxygen Species.

References





DOI: https://doi.org/10.18520/cs%2Fv120%2Fi6%2F1088-1091