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Immunocytochemical localization of leptin hormone in the neurosecretory cells of brain–suboesophageal ganglion complex of tropical tasar silkworm, Antheraea mylitta (D.) eco-race Bhandara


Affiliations
1 P.G. Department of Zoology, MJF Educational Campus, RTM Nagpur University, Nagpur 440 033, India
2 Department of Zoology, R. S. Bidkar Arts, Commerce and Science College, Hinganghat Dist., Wardha 442 301, India
3 Department of Zoology, Nabira Mahavidyalaya, Katol Dist., Nagpur 441 302, India
 

Leptin is a peripheral agent known for its function in feeding behaviour in vertebrates. In this study, we demonstrated leptin immunoreactivity in the brain and suboesophageal ganglion (SOG) of tropical tasar silkworm, Antheraea mylitta (D.) by immunohistochemistry using polyclonal antibody against mammalian leptin. Leptin immunoreactivity was not observed in the adult brain, whereas, intense reactivity was detected in the single neuronal group of cells in SOG. This study provides an essential groundwork to further elucidate the involvement of leptin in insect development and appetite regulatory systems of tropical tasar silkworm, Antheraea mylitta (D.) as well as in other insects.

Keywords

Antheraea mylitta, leptin, suboesophageal ganglion, tasar silkworm.
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  • Immunocytochemical localization of leptin hormone in the neurosecretory cells of brain–suboesophageal ganglion complex of tropical tasar silkworm, Antheraea mylitta (D.) eco-race Bhandara

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Authors

Deepak D. Barsagade
P.G. Department of Zoology, MJF Educational Campus, RTM Nagpur University, Nagpur 440 033, India
Shruti A. Gharade
Department of Zoology, R. S. Bidkar Arts, Commerce and Science College, Hinganghat Dist., Wardha 442 301, India
Vikas G. Barsagade
Department of Zoology, Nabira Mahavidyalaya, Katol Dist., Nagpur 441 302, India

Abstract


Leptin is a peripheral agent known for its function in feeding behaviour in vertebrates. In this study, we demonstrated leptin immunoreactivity in the brain and suboesophageal ganglion (SOG) of tropical tasar silkworm, Antheraea mylitta (D.) by immunohistochemistry using polyclonal antibody against mammalian leptin. Leptin immunoreactivity was not observed in the adult brain, whereas, intense reactivity was detected in the single neuronal group of cells in SOG. This study provides an essential groundwork to further elucidate the involvement of leptin in insect development and appetite regulatory systems of tropical tasar silkworm, Antheraea mylitta (D.) as well as in other insects.

Keywords


Antheraea mylitta, leptin, suboesophageal ganglion, tasar silkworm.

References





DOI: https://doi.org/10.18520/cs%2Fv120%2Fi10%2F1611-1615