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Transcriptomic Response of Caenorhabditis elegans Expressing Human Aβ42 Gene Treated with Salvianolic Acid A


Affiliations
1 School of Biological Sciences, Universiti Sains Malaysia, 11800 Penang, Malaysia
2 USM-RIKEN International Centre for Ageing Science, Universiti Sains Malaysia, 11800 Penang, Malaysia
 

Alzheimer’s disease is associated with the deposition of β-amyloid peptide in the brain. A genome-wide transcriptomic study was performed to determine the response of transgenic Caenorhabditis elegans express-ing full-length human Aβ42 gene towards salvianolic acid A (Sal A). The genes associated with antioxidant response, gst-4, gst-10, spr-1 and trxr-2, were upregu-lated. Aβ42 caused oxidative stress and the antioxidant response genes possibly provide some sort of protec-tion to the nematode. trxr-2 gene product was also associated with the defence system and probably has a role in the lifespan of the nematode. Other genes involved in DNA replication, reproduction, immune res-ponse and antimicrobial activities were also found to be upregulated. Treatment of Sal A also increased the rate of reproduction in the nematode, and elevated its immunological protection system towards microor-ganisms. On the other hand, the genes responsible for ligand-gated cation channel, embryonic and postem-bryonic development, locomotion and neuromodula-tion of chemosensory neurons were found to be down-regulated. As an effector, Sal A might conceivably reduce the movement of the nematode by interfering with neuronal transmission, and embryonic and post-embryonic development.

Keywords

β-Amyloid Peptide, Caenorhabditis elegans, Salvianolic Acid A, Transcriptome.
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  • Transcriptomic Response of Caenorhabditis elegans Expressing Human Aβ42 Gene Treated with Salvianolic Acid A

Abstract Views: 275  |  PDF Views: 125

Authors

Chee Wah Yuen
School of Biological Sciences, Universiti Sains Malaysia, 11800 Penang, Malaysia
Mardani Abdul Halim
USM-RIKEN International Centre for Ageing Science, Universiti Sains Malaysia, 11800 Penang, Malaysia
Nazalan Najimudin
School of Biological Sciences, Universiti Sains Malaysia, 11800 Penang, Malaysia
Ghows Azzam
School of Biological Sciences, Universiti Sains Malaysia, 11800 Penang, Malaysia

Abstract


Alzheimer’s disease is associated with the deposition of β-amyloid peptide in the brain. A genome-wide transcriptomic study was performed to determine the response of transgenic Caenorhabditis elegans express-ing full-length human Aβ42 gene towards salvianolic acid A (Sal A). The genes associated with antioxidant response, gst-4, gst-10, spr-1 and trxr-2, were upregu-lated. Aβ42 caused oxidative stress and the antioxidant response genes possibly provide some sort of protec-tion to the nematode. trxr-2 gene product was also associated with the defence system and probably has a role in the lifespan of the nematode. Other genes involved in DNA replication, reproduction, immune res-ponse and antimicrobial activities were also found to be upregulated. Treatment of Sal A also increased the rate of reproduction in the nematode, and elevated its immunological protection system towards microor-ganisms. On the other hand, the genes responsible for ligand-gated cation channel, embryonic and postem-bryonic development, locomotion and neuromodula-tion of chemosensory neurons were found to be down-regulated. As an effector, Sal A might conceivably reduce the movement of the nematode by interfering with neuronal transmission, and embryonic and post-embryonic development.

Keywords


β-Amyloid Peptide, Caenorhabditis elegans, Salvianolic Acid A, Transcriptome.

References





DOI: https://doi.org/10.18520/cs%2Fv120%2Fi12%2F1882-1893