Current Science

Open Access Open Access  Restricted Access Subscription Access

Unexplored Pharmaceutical Potential of Phytocompounds Extracted from The Mushroom, Geastrum saccatum


Affiliations
1 P.G. Department of Zoology and Research Centre, Shri Shiv Chhatrapati College of Arts, Commerce and Science, Junnar, India
2 Chandrapur Forest Academy, Mul Road, Chandrapur 442 401, India
 

The phytochemical content and medicinal properties of the mushroom Geastrum saccatum, collected from the northern Western Ghats were evaluated. The mushroom powder was extracted in different solvents separately and assessed for the presence of phytochemicals. Anti-inflammatory, anti-diabetic, antioxidant and iron chelating activities of the mushroom extract were evaluated. The results revealed that chloroform extract of G. saccatum (CEGS) exhibited the maximum number of phytochemicals compared to the other extracts and hence was selected for further studies. The mushroom analysed contains different types of phytoconstituents having pharmaceutical activities. Maximum activity for the studied bioassays was found at 50 μg/ml of CEGS concentration. Thus chloroform extract of G. saccatum has potential pharmaceutical properties and thus can be used for the treatment of different diseases.

Keywords

Chloroform Extract, Medicinal Properties, Mushroom, Pharmaceutical Potential, Phytoconstituents.
User
Notifications
Font Size

  • Zaidman, B., Yassin, M., Mahajana, J. and Wasser, S. P., Medicinal mushrooms modulators of molecular targets as cancer therapeutics. Appl. Microbial. Biotechnol., 2005, 67, 453–468.

  • Stamets, P., Growing Gourmet and Medicinal Mushrooms, Ten Speed Press, California, 2000, p. 574.

  • Cohen, R., Persky and Hadar, Y., Biotechnological applications and potential of wood-degrading mushrooms of the genus Pleurotus. Appl. Microbial. Biotechnol., 2002, 58, 582–594.

  • Kupra, J., Anke, T., Oberwinkler, G., Schramn, G. and Steglich, W., Antibiotics from basidiomycetes VII. Crinipelliss tripitaria (Fr.) Pat. J. Antibiot., 1979, 32, 130–135.

  • Wasser, S. P., Medicinal mushrooms as a source of anti-tumour and immunomodulating polysaccharides. Appl. Microbial. Biotechnol., 2002, 60, 258–274.

  • Hawksworth, D. L., Mushrooms the extent of the unexplored potential. Int. J. Med. Mush., 2001, 3, 333–340.

  • Pushpa, H. and Purushothama, K. B., Biodiversity of mushrooms in and around Bangalore (Karnataka), India. Am. Eur. J. Agric. Environ. Sci., 2012, 12, 750–759.

  • Sundberg, W. and Bessette, A., Mushrooms: A Quick Reference Guide to Mushrooms of North America (Macmillan Field Guides), Collier Books, New York, 1987, p. 20.

  • Shahidi, F., Maximizing the Value of Marine By-Products, Woodhead Publishing Ltd, Cambridge, UK, 2006, p. 460.

  • Romulo, R. N. A. and Ierece, R., Why study the use of animal products in traditional medicines. J. Ethnobiol. Ethnomed., 2005, 1, 1–5.

  • Isabel, M. S., Ricardo, F., Nuria, A., Dolores, M. M., Pilar, S. and Francisco, B. M., Antioxidant and cytotoxic activities of three edible fungi (Tricholoma spp.) on tumor cells. EC Agric., 2018, 4(1), 62–69.

  • Chaturvedi, V. K., Dubey, S. K. and Singh, M. P., Antidiabetic Potential of medicinal mushrooms. In Phytochemicals from Medicinal Plants Scope, Applications, and Potential Health Claims, AAP & CRC Press, New Jersey, USA, 2019; doi:10.1201/9780429203220-7.

  • Katarzyna, W., Wanda, M., Klaudia, G. and Małgorzata, G., Mushrooms of the genus ganoderma used to treat diabetes and insulin resistance. Molecules, 2019, 24, 4075; doi:10.3390/molecules24224075.

  • Jayachandran, M., Wu, Z., Ganesan, K., Khalid, S., Chung, S. M. and Xu, B., Isoquercetin upregulates antioxidant genes, suppresses inflammatory cytokines and regulates AMPK pathway in streptozotocininduced diabetic rats. Chem. Biol. Interact, 2019, 303, 62–69; doi:10.1016/j.cbi.2019.02.017.

  • Largent, D. L., How to Identify Mushrooms to Genus I: Macroscopial Features, Mad Rivers Press, Eureka, USA, 1977, pp. 1–85.

  • Fransworth, N. R., Akerele, O. and Bingel, A. S., Medicinal plants in therapy. Bull. World Health Organ., 1985, 63, 965–981.

  • Lettered, G. D., Ismail, A., Basher, R. H. and Bahrain, H. M., Antimicrobial effects of Sodium guajava extract as one mechanism of its anti diarrhoeal action. Malaysian J. Med. Sci., 1999, 6, 17–20.

  • Marjorie, M. C., Plant products as antimicrobial agents. Clin. Microbial. Rev., 1999, 12, 564–582.

  • Weisser, R., Asscher, A. W. and Winpenny, J., In vitro reversal of antibiotic resistance by DTA. Nature, 1966, 219, 1365–1366.

  • Ogbulie, I. N., Ogueke, C. C. and Nwanebu, F. C., Antibacterial properties of Uvaria chamae, Congronema latifolium, Garcinia kola, Vemonia amygdalina and Aframomium melegueta. Afr. J. Biotech., 2007, 6, 1549–1553.

  • Shirwaikar, A., Govindrajan, R., Rastogi, S., Vijaykumar, M., Rawat, A. K. S. and Ehlotra, S. M., Studies on the antioxidant activities of Desmodium gangeticum. Biol. Pharm. Bull., 2003, 26, 1424–1427.

  • Benzie, I. F. and Szeto, Y. T., Total antioxidant capacity of teas by the ferric reducing antioxidant power assay. J. Agric. Food Chem., 1999, 47, 633–636.

  • Chandra, S., Chatterjee, P., Dey, P. and Bhattacharya, S., Evaluation of in vitro anti-inflammatory activity of coffee against the denaturation of protein. Asian J. Trop. Biomed., 2012, 2, 5178–5180.

  • Williams, L. A. D. et al., The in vitro anti-denaturation effects induced by natural products and non-steroidal compounds in heat treated (immunogenic) bovine serum albumin is proposed as a screening assay for the detection of anti-inflammtory compounds, without the use of animals, in the early stages of the dung discovery process. West Indian Med. J., 2008, 57, 327.

  • Johann, S., Pizzolotti, M. G., Donnici, C. L. and De Resend, M. A., Antifungal properties of plants used in Brazilian traditional medicine against clinically relevant fungal pathogens. Braz. J. Microbiol., 2007, 38, 632–637.

  • Sanmee, R., Dell, B., Lumyong, P., Izumori, K. and Lumyong, S., Nutritive value of popular wild edible mushrooms from Northern Thailand. Food Chem., 2003, 82, 527–532.

  • Wang, G. Y., Tang, W. P. and Bidigare, R. R., Terpenoids as therapeutic drugs and pharmaceutical agents. In Natural Products (eds Zhang, L. X. and Demain, A. L.), Humana Press, New Jersey, USA, 2005, pp. 197–227.

  • Zhou, Z., Lin, J., Yin, Y., Zhao, J., Sun, X. and Tang, K., Ganodermataceae: Natural products and their related pharmacological functions. Am. J. Chin. Med., 2007, 35, 559–574.

  • Nathan, C., Points of control in inflammation. Nature, 2002, 420, 846–852.

  • Coussens, L. M. and Werb, Z., Inflammation and cancer. Nature, 2002, 420, 860-867.

  • Dudhgaonkar, S., Thyagarajan, A. and Silva, D., Suppression of the inflammatory response by triterpenes isolated from the mushroom Ganoderma lucidum. Int. Immunopharmacol., 2009, 9, 1272–1280.

  • Witkowska, A. M., Zujko, M. E. and Mironczuk–Chodakowaska, I., Comparative study of wild edible mushrooms as sources of antioxidants. Int. J. Med. Mush., 2011, 13, 335–341.

  • Just, M. J., Racio, M. C., Giner, R. M., Cuellar, M. J., Manez, S., Bilia, A. R. and Rios, J. L., Anti-inflammatory activity of unusual Jupane saponins from Bupleurum Fruticescebce. Plantamedica, 1998, 64, 404–407.

  • Borchers, A. T., Krishamurthy, A., Keen, C. L., Meyers, F. J. and Gershwin, M. E., The immunobiology of mushrooms. Exp. Biol. Med. (Maywood), 2008, 233, 259–276.

  • Lull, C., Wichers, H. J. and Savelkoul, H. F. J., Anti-inflammatory and immunomodulating properties of fungal metabolites. Mediatorsinflamm, 2005, 2, 63–80.

  • Bailli, J. K. et al., Oral antioxidant supplement does not prevent acute mountain sickness: double blind, randomized placebocontrolled trial. QJM, Int. J. Med., 2009, 102, 341–348.

  • Ozen, T., Daecan, C., Actop, O. and Turkekul, I., Screening of antioxidant, antimicrobial activities and chemical contents of edible mushrooms widely grown in the Black sea region of Turkey. Combinatorial Chem. High Through Put Screening, 2011, 14, 72–84.

  • Ribeiro, B. R., Lopes, P. B., Andrade, R. M., Seabra, R. F., Goncaves, P., Baptisa, I. and Valentao, P., Comparative study of phytochemicals and antioxidant potential of wild edible mushroom Caps and Stipes. Food Chem., 2008, 110, 47–56.

  • Sawa, T., Nakao, M., Akaike, T., Ono, K. and Maeda, H., Alkyl peroxyl radical scavenging activity of various flavonoids and other phenolic compounds: Implications for the anti-tumour promoter effect of vegetables. J. Agric. Food Chem., 1999, 47, 397–492.

  • Ghafar, M. F. A., Nagendra, P. K., Weeng, K. K. and Ismail, A., Flavonoid, Hesperidine, total phenolic contents and anti-oxidant activities from Citrus species. Afr. J. Biotechnol., 2010, 9, 326–330.

  • Evans, R. C. A., Miller, N. I. and Paganga, G., Structure –antioxidant activity relationships of flavonoids and phenolic acids. Free Radic. Biol. Med., 1996, 20, 933.

  • Buxiang, S. and Fukuhara, M., Effects of coadministration of butylated hydroxyl toluene, butylated hydroxyl anisole and flavonoid on the activation of mutagens and drug-metabolizing enzymes in mice. Toxicology, 1997, 12, 61–72.

  • Monika, G., Zuzanna, M., Marek, S. and Mirosław, M., Profile of phenolic and organic acids, antioxidant properties and ergosterol content in cultivated and wild growing species of Agaricus. Eur. Food Res. Technol., 2018, 244, 259–268; https://doi.org/10.1007/s00217-017-2952-9.

  • Duh, P. D., Tu, Y. Y. and Yen, G. C., Antioxidant activity of water extract of Harng jyur, Chrysanthemum morifolium Ramat. Lebensmittel-Wissenchaft Technol., 1999, 32, 269–277.

  • Pal, J., Ganguly, S., Tahsin, K. S. and Acharya, K., In vitro Free radical scavenging activity of wild edible mushroom, Pleurotus squarrosulus (mont) Singer. Indian J. Exp. Biol., 2010, 47, 1210–1218.

  • Reher, G., Slijepcevic, M. and Krans, L., Hypoglycemic activity of triterpenes and tannins from Sarcopoterium spinosum and two Sanguisorba species. Planta Med., 1991, 57, A57–A58.

  • Lee, J., Lim, S., Kang, S. M., Min, S., Son, K. and Lee, H. S., Saponin inhibits Hepatitis C virus propagation by up-regulating suppressor of cytokine signaling. PLoS ONE, 2012; doi:10.1371/ journal.

  • Marles, R. and Fransworth, N., Antidiabetic plants and their active constituents. Phytomedicine, 1995, 2, 137–165.

  • Grover, J. K., Yadav, S. and Vars, V., Medicinal plants of India with hypoglycemic potentials. J. Ethnopharmacol., 2002, 81, 81–100.

  • Reher, G., Slijepcevic, M. and Krans, L., Hypoglycemic activity of triterpenes and tannins from Sarcopoterium spinosum and two Sanguisorba species. Planta Med., 1991, 57, A57–A58.


Abstract Views: 330

PDF Views: 118




  • Unexplored Pharmaceutical Potential of Phytocompounds Extracted from The Mushroom, Geastrum saccatum

Abstract Views: 330  |  PDF Views: 118

Authors

Pramod C. Mane
P.G. Department of Zoology and Research Centre, Shri Shiv Chhatrapati College of Arts, Commerce and Science, Junnar, India
Ashok N. Khadse
Chandrapur Forest Academy, Mul Road, Chandrapur 442 401, India
Deepali D. Kadam
P.G. Department of Zoology and Research Centre, Shri Shiv Chhatrapati College of Arts, Commerce and Science, Junnar, India
Shabnam A. R. Sayyed
P.G. Department of Zoology and Research Centre, Shri Shiv Chhatrapati College of Arts, Commerce and Science, Junnar, India
Vrushali T. Thorat
P.G. Department of Zoology and Research Centre, Shri Shiv Chhatrapati College of Arts, Commerce and Science, Junnar, India
Sunita D. Sarogade
P.G. Department of Zoology and Research Centre, Shri Shiv Chhatrapati College of Arts, Commerce and Science, Junnar, India
Ravindra D. Chaudhari
P.G. Department of Zoology and Research Centre, Shri Shiv Chhatrapati College of Arts, Commerce and Science, Junnar, India

Abstract


The phytochemical content and medicinal properties of the mushroom Geastrum saccatum, collected from the northern Western Ghats were evaluated. The mushroom powder was extracted in different solvents separately and assessed for the presence of phytochemicals. Anti-inflammatory, anti-diabetic, antioxidant and iron chelating activities of the mushroom extract were evaluated. The results revealed that chloroform extract of G. saccatum (CEGS) exhibited the maximum number of phytochemicals compared to the other extracts and hence was selected for further studies. The mushroom analysed contains different types of phytoconstituents having pharmaceutical activities. Maximum activity for the studied bioassays was found at 50 μg/ml of CEGS concentration. Thus chloroform extract of G. saccatum has potential pharmaceutical properties and thus can be used for the treatment of different diseases.

Keywords


Chloroform Extract, Medicinal Properties, Mushroom, Pharmaceutical Potential, Phytoconstituents.

References





DOI: https://doi.org/10.18520/cs%2Fv120%2Fi12%2F1917-1922