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Characterization of granulosis viruses of sugarcane early shoot borer, Chilo infuscatellus (Snell.) and internode borer, Chilo sacchariphagus indicus (Kapur)


Affiliations
1 Nano Science and Technology, Tamil Nadu Agricultural University, Coimbatore 641 003, India
2 ICAR-Sugarcane Breeding Institute, Coimbatore 641 007, India
3 Imayam Institute of Agriculture and Technology, Tiruchirappalli 621 206, India
4 ICAR-National Bureau of Agricultural Insect Resources, Hebbal, Bengaluru 560 024, India
 

A study was undertaken to characterize the granulosis viruses (GVs) of early shoot borer, Chilo infuscatellus (Snell.) (Crambidae: Lepidoptera) and internode borer, Chilo sacchariphagus indicus (Kapur) (Lepidoptera: Crambidae) in sugarcane. Scanning electron photomicrographs revealed ovo-cylindrical occlusion bodies (OBs) of GVs in early shoot and internode borers with an average size of 425.03 and 230.21 nm, 387.64 and 208.68 nm in length and breadth respectively. Transmission electron photomicrographs also showed ovo-cylindrical OBs embedded with a rod-shaped virion. The average length and breadth of the virion in the OB was 271.0 ´ 52.6 nm, 257.0 ´ 50.2 nm for early shoot and internode borer GVs respectively. Toxicity studies with the respective GVs revealed lethal concentration values of 4.38, 4.61, 6.89 OBs/mm2 and 1.85, 135.43, 8045.27 OBs/mm2 to second, third and fourth larval instars of Chilo infuscatellus granulosis virus (ChinGV) and Chilo sacchariphagus indicus granulosis virus (ChsaGV) respectively.

Keywords

Early shoot borer, granulosis viruses, internode borer, occlusion bodies, sugarcane, toxicity.
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  • Characterization of granulosis viruses of sugarcane early shoot borer, Chilo infuscatellus (Snell.) and internode borer, Chilo sacchariphagus indicus (Kapur)

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Authors

M. Kannan
Nano Science and Technology, Tamil Nadu Agricultural University, Coimbatore 641 003, India
N. Geetha
ICAR-Sugarcane Breeding Institute, Coimbatore 641 007, India
K. Elango
Imayam Institute of Agriculture and Technology, Tiruchirappalli 621 206, India
M. Mohan
ICAR-National Bureau of Agricultural Insect Resources, Hebbal, Bengaluru 560 024, India
G. Sivakumar
ICAR-National Bureau of Agricultural Insect Resources, Hebbal, Bengaluru 560 024, India

Abstract


A study was undertaken to characterize the granulosis viruses (GVs) of early shoot borer, Chilo infuscatellus (Snell.) (Crambidae: Lepidoptera) and internode borer, Chilo sacchariphagus indicus (Kapur) (Lepidoptera: Crambidae) in sugarcane. Scanning electron photomicrographs revealed ovo-cylindrical occlusion bodies (OBs) of GVs in early shoot and internode borers with an average size of 425.03 and 230.21 nm, 387.64 and 208.68 nm in length and breadth respectively. Transmission electron photomicrographs also showed ovo-cylindrical OBs embedded with a rod-shaped virion. The average length and breadth of the virion in the OB was 271.0 ´ 52.6 nm, 257.0 ´ 50.2 nm for early shoot and internode borer GVs respectively. Toxicity studies with the respective GVs revealed lethal concentration values of 4.38, 4.61, 6.89 OBs/mm2 and 1.85, 135.43, 8045.27 OBs/mm2 to second, third and fourth larval instars of Chilo infuscatellus granulosis virus (ChinGV) and Chilo sacchariphagus indicus granulosis virus (ChsaGV) respectively.

Keywords


Early shoot borer, granulosis viruses, internode borer, occlusion bodies, sugarcane, toxicity.

References





DOI: https://doi.org/10.18520/cs%2Fv121%2Fi4%2F570-573