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Genome wide analysis of 14-3-3 proteins in Cicer arietinum L. and identification of isoforms responsive to Fusarium oxysporum


Affiliations
1 Plant Molecular Biology Unit, Division of Biochemical Sciences, CSIR-National Chemical Laboratory, Pune 411 008, India; Department of Biotechnology, Fergusson College (Autonomous), Pune 411 004, India, India
2 Plant Molecular Biology Unit, Division of Biochemical Sciences, CSIR-National Chemical Laboratory, Pune 411 008, India; Eitimo Ventures, LLP, No. 22, 7th Main, 8th Cross, Malleshwaram, Bengaluru 560 003, India, India
3 Plant Molecular Biology Unit, Division of Biochemical Sciences, CSIR-National Chemical Laboratory, Pune 411 008, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201 002, India, India
 

In the present study, we have identified and characterized two 14-3-3 isoforms, namely isoform A and C from chickpea (Cicer arietinum L.), that might play a crucial role during disease resistance. Further, in silico analysis of these 14-3-3 proteins was accomplished, including motif identification and structure prediction from deduced amino acid sequences. Expression profiling of the two representative 14-3-3 isoforms in the ischolar_mains of wilt resistant and susceptible chickpea varieties upon Fusarium oxysporum f. sp ciceri race 1 (FOC1) challenge, revealed time dependent isoform specific differential expression in induced chickpea ischolar_mains upon FOC1 colonization

Keywords

Bioinformatics, chickpea 14-3-3s, pathogen responsive, transcriptional analysis.
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  • Genome wide analysis of 14-3-3 proteins in Cicer arietinum L. and identification of isoforms responsive to Fusarium oxysporum

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Authors

Gayatri Gurjar
Plant Molecular Biology Unit, Division of Biochemical Sciences, CSIR-National Chemical Laboratory, Pune 411 008, India; Department of Biotechnology, Fergusson College (Autonomous), Pune 411 004, India, India
Suhas Nimbalkar
Plant Molecular Biology Unit, Division of Biochemical Sciences, CSIR-National Chemical Laboratory, Pune 411 008, India; Eitimo Ventures, LLP, No. 22, 7th Main, 8th Cross, Malleshwaram, Bengaluru 560 003, India, India
Ashok Giri
Plant Molecular Biology Unit, Division of Biochemical Sciences, CSIR-National Chemical Laboratory, Pune 411 008, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201 002, India, India
Vidya Gupta
Plant Molecular Biology Unit, Division of Biochemical Sciences, CSIR-National Chemical Laboratory, Pune 411 008, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201 002, India, India

Abstract


In the present study, we have identified and characterized two 14-3-3 isoforms, namely isoform A and C from chickpea (Cicer arietinum L.), that might play a crucial role during disease resistance. Further, in silico analysis of these 14-3-3 proteins was accomplished, including motif identification and structure prediction from deduced amino acid sequences. Expression profiling of the two representative 14-3-3 isoforms in the ischolar_mains of wilt resistant and susceptible chickpea varieties upon Fusarium oxysporum f. sp ciceri race 1 (FOC1) challenge, revealed time dependent isoform specific differential expression in induced chickpea ischolar_mains upon FOC1 colonization

Keywords


Bioinformatics, chickpea 14-3-3s, pathogen responsive, transcriptional analysis.

References





DOI: https://doi.org/10.18520/cs%2Fv121%2Fi8%2F1039-1045