Current Science

Open Access Open Access  Restricted Access Subscription Access

Factors involved in enhancing host susceptibility towards aphid clonal propagation on leaf foliage of Arabidopsis


Affiliations
1 Genetics and Plant Breeding, Uttar Banga Krishi Viswavidyalaya, Cooch Behar 736 165, India; Present address: School of Crop Improvement, College of Post Graduate Studies in Agricultural Sciences, Umiam 793 103, India, India
2 Genetics and Plant Breeding, Uttar Banga Krishi Viswavidyalaya, Cooch Behar 736 165, India, India
 

The present study identified factors that enhanced host susceptibility towards Myzus persicae’s clonal proliferation in the model plant, Arabidopsis thaliana. A particular aphid inoculum, i.e. five aphids release per plant among three inoculums (1, 5 and 10 aphids per plant) showed enhanced susceptibility towards aphid clonal propagation in 21-day-old Arabidopsis leaf foliage. Five aphid number was common among the 28, 35, 42, 49-day-old Arabidopsis leaf foliage except 42-day-old Arabidopsis. Prior aphid herbivory enhan­ced host susceptibility in Arabidopsis. The aphid ino­culum at 6 am showed enhanced host susceptibility in comparison to 12 noon and 6 pm. The enhanced susceptibility on leaf foliage was realized in the presence of flower stalk. Aphid preferred to proliferate significantly on the flower stalk as compared to leaf foliage. Within leaf, aphid colonized more in mid-rib region as compared to minor vein area in the mature leaf. ProPAD4 :: GUS and ProADF3 :: GUS showed poor expression in mid-rib region in aphid-challenged leaf foliage. The aphid feeding based primed vascular sap showed degraded peptide bond, a possible reason for favouring enhanced aphid clonal proliferation in the primed Arabidopsis leaf foliage. Results showed that the enhancing of host susceptibility in Arabidopsis to Myzus persicae is influenced by quorum number of aphid’s inoculum, prior aphid feeding and circadian rhythms. Differential spatial resistance within whole plant and within leaf was also recorded. The enhanced host susceptibility was also correlated with microbiota enrichment in aphid-herbivore leaf vasculature sap as well as aphid body including aphid apical part containing salivary gland

Keywords

Arabidopsis thaliana, aphid inoculums, enhanced host susceptibility, spatial resistance, timing of aphid inoculums.
User
Notifications
Font Size

  • Doring, T. F., How aphids find their host plants, and how they don’t. Annu. Appl. Biol., 2014, 165, 3–26.

  • Doring, T. F. and Chittka, L., Visual ecology of aphids – a critical review on the role of colours in host finding. Arthropod–Plant Interact., 2007, 1, 316.

  • Sengupta, S., Chakraborti, D., Mondal, H. A. and Das, S., Selectable antibiotic resistance marker gene-free transgenic rice harbouring the garlic leaf lectin gene exhibits resistance to sap-sucking plant hoppers. Plant Cell Rep., 2010, 29(3), 261–271.

  • Neal, J. J., Tingey, W. M. and Steffens, J. C., Sucrose esters of carboxylic-acids in glandular trichomes of Solanumber thaultii deter settling and probing by green peach aphid. J. Chem. Ecol., 1990, 16, 487–497.

  • Chakraborti, D., Sarkar, A., Mondal, H. A. and Das, S., Tissue specific expression of Alliumsativum leaf agglutinin (ASAL) in important pulse crop chickpea (Cicerarietinum L.) to resist the phloem feeding Aphiscraccivora. Trans. Res., 2009, 18, 529–544.

  • Mondal, H. A., Roy, A., Gupta, S. and Das, S., Exploring the insecticidal potentiality of Amorphophallus paeonifolius tuber agglutinin in hemipteran pest management. Am. J. Plant Sci., 2012, 3, 780–790; doi:10.4236/ajps.2012.36094.

  • Jaouannet, M., Morris, J. A., Hedley, P. E. and Bos, J. I., Characterization of Arabidopsis transcriptional responses to different aphid species reveals genes that contribute to host susceptibility and non-host resistance. PLOS Pathog., 2015, 11, e1004918.

  • Debokx, J. A. and Piron, P. G. M., Relative efficiency of a number of aphid species in the transmission of potato virus-YN in the Netherlands. Netherlands J. Plant Pathol., 1990, 96, 237–246.

  • Katis, N. and Gibson, R. W., Transmission of potato virus-Y by cereal aphids. Potato Res., 1985, 28, 65–70.

  • Verbeek, M., Piron, P. G. M., Dullemans, A. M., Cuperus, C. and Van Der Vlugt, R. A. A., Determination of aphid transmission efficiencies for N, NTN and Wilga strains of Potato virus Y. Ann. Appl. Biol., 2010, 156, 39–49.

  • Tjallingii, W. F. and Esch, T. H., Fine structure of aphid stylet routes in plant tissues in correlation with EPG signals. Physiol. Entomol., 1993, 18, 313–328.

  • Tjallingii, W. F., Salivary secretions by aphids interacting with proteins of phloem wound responses. J. Exp. Bot., 2006, 57, 739– 745.

  • Tjallingii, W. F., Aphid-plant interactions: what goes on in the depth of the tissues? In Proceedings of the Section Experimental and Applied Entomology of the Netherlands Entomological Society (NEV), 1995, vol. 6, pp. 163–169.

  • Louis, J., Singh, V. and Shah, J., Arabidopsis thaliana – aphid interaction. Arabidopsis Book, 2012, vol. 10, e0159.

  • Chakraborti, D., Sarkar, A., Mondal, H. A., Schuermann, D., Hohn, B., Sarmah, B. K. and Das, S., Cre/lox system to develop selectable marker free transgenic tobacco plants conferring resistance against sap sucking homopteran insects. Plant Cell Rep., 2008, 27, 1623–1633.

  • Saha, P., Majumder, P., Dutta, I., Ray, T., Roy, S. C. and Das, S., ransgenic rice expressing Alliumsativum leaf lectin with enhanced resistance against sap-sucking insect pests. Planta, 2006, 223, 1329–1343; https://doi.org/10.1007/s00425-005-0182-z.

  • Louis, J., Lorenc-Kukula, K., Singh, V., Reese, J., Jander, G. and Shah, J., Antibiosis against the green peach aphid requires the Arabidopsis thaliana myzuspersicae-induced lipase 1 gene. Plant J., 2010, 64, 800–811.

  • Louis, J., Leung, Q., Pegadaraju, V., Reese, J. and Shah, J., PAD4-dependent antibiosis contributes to the ssi2-conferred hyper resistance to the green peach aphid. Mol. Plant–Microbe Interact., 2010, 23, 618–627.

  • Louis, J., Gobbato, E., Mondal, H. A., Feys, B. J., Parker, J. E. and Shah, J., Discrimination of Arabidopsis PAD4 activities in defense against green peach aphid and pathogens. Plant Physiol., 2012, 158, 1860–1872.

  • Majumder, P., Mondal, H. A. and Das, S., Insecticidal activity of Arummaculatum tuber lectin and its binding to the glycosylated insect gut receptors. J. Agric. Food Chem., 2005, 53, 6725–6729.

  • Nealson, K. H., Platt, T. and Hastings, J. W., Cellular control of the synthesis and activity of the bacterial luminescent system. J. Bacteriol., 1970, 104, 313–322.

  • Rutherford, S. T. and Bassler, B. L., Bacterial quorum sensing: its role in virulence and possibilities for its control. Cold Spring Harb. Perspect. Med., 2012, 2, 1–25; doi:10.1101/cshperspect.a012427.

  • Sifri, C. D., Quorum sensing: bacteria talk sense. Clin. Infect. Dis., 2008, 47, 1070–1076; doi:10.1086/592072.

  • Furch, A. C., van Bel, A. J. and Will, T., Aphid salivary proteases are capable of degrading SE-tube proteins. J. Exp. Bot., 2015, 66, 533–539; doi:10.1093/jxb/eru487.

  • Skaljac, M., Vogel, H., Wielsch, N., Mihajlovic, S. and Vilcinskas, A., Transmission of a protease – secreting bacterial symbiont among pea aphids via host plants. Front. Physiol., 2019, 10, 438;doi:10.3389/fphys.2019.00438.

  • Das, A., Roy, A., Mandal, A., Mondal, H. A., Hess, D., Kundu, P. and Das, S., Inhibition of Bemisiatabaci vectored, GroEL mediated transmission of tomato leaf curl New Delhi virus by garlic leaf lectin (Allium sativum leaf agglutinin). Virus Res., 300, 2021, 198443.


Abstract Views: 408

PDF Views: 129




  • Factors involved in enhancing host susceptibility towards aphid clonal propagation on leaf foliage of Arabidopsis

Abstract Views: 408  |  PDF Views: 129

Authors

Hossain Ali Mondal
Genetics and Plant Breeding, Uttar Banga Krishi Viswavidyalaya, Cooch Behar 736 165, India; Present address: School of Crop Improvement, College of Post Graduate Studies in Agricultural Sciences, Umiam 793 103, India, India
Bablu Paul
Genetics and Plant Breeding, Uttar Banga Krishi Viswavidyalaya, Cooch Behar 736 165, India, India
Albina Gurung
Genetics and Plant Breeding, Uttar Banga Krishi Viswavidyalaya, Cooch Behar 736 165, India, India
Moumita Mallick
Genetics and Plant Breeding, Uttar Banga Krishi Viswavidyalaya, Cooch Behar 736 165, India, India

Abstract


The present study identified factors that enhanced host susceptibility towards Myzus persicae’s clonal proliferation in the model plant, Arabidopsis thaliana. A particular aphid inoculum, i.e. five aphids release per plant among three inoculums (1, 5 and 10 aphids per plant) showed enhanced susceptibility towards aphid clonal propagation in 21-day-old Arabidopsis leaf foliage. Five aphid number was common among the 28, 35, 42, 49-day-old Arabidopsis leaf foliage except 42-day-old Arabidopsis. Prior aphid herbivory enhan­ced host susceptibility in Arabidopsis. The aphid ino­culum at 6 am showed enhanced host susceptibility in comparison to 12 noon and 6 pm. The enhanced susceptibility on leaf foliage was realized in the presence of flower stalk. Aphid preferred to proliferate significantly on the flower stalk as compared to leaf foliage. Within leaf, aphid colonized more in mid-rib region as compared to minor vein area in the mature leaf. ProPAD4 :: GUS and ProADF3 :: GUS showed poor expression in mid-rib region in aphid-challenged leaf foliage. The aphid feeding based primed vascular sap showed degraded peptide bond, a possible reason for favouring enhanced aphid clonal proliferation in the primed Arabidopsis leaf foliage. Results showed that the enhancing of host susceptibility in Arabidopsis to Myzus persicae is influenced by quorum number of aphid’s inoculum, prior aphid feeding and circadian rhythms. Differential spatial resistance within whole plant and within leaf was also recorded. The enhanced host susceptibility was also correlated with microbiota enrichment in aphid-herbivore leaf vasculature sap as well as aphid body including aphid apical part containing salivary gland

Keywords


Arabidopsis thaliana, aphid inoculums, enhanced host susceptibility, spatial resistance, timing of aphid inoculums.

References





DOI: https://doi.org/10.18520/cs%2Fv121%2Fi8%2F1080-1089