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Dynamic Three-Dimensional Cell-Culture Systems for Enhanced in Vitro Applications


Affiliations
1 Department of Human Genetics, Faculty of Biomedical Sciences, Technology and Research, Sri Ramachandra Institute of Higher Education and Research, Chennai 600 116, India
 

The advent of dynamic three-dimensional cell cultures has transformed the field of biological research as they bridge the gap between in vitro and in vivo systems. 3D cell-culture techniques can be categorized into two types: static and dynamic culture systems. Traditional cell-culture models are considered to be ‘static’ in nature, as the cells are grown on matrices or scaffolds with little focus given to the complexity of the growth conditions that exist in the in vivo tissue microenvironments (presence of continuous blood supply for the development of tumour vasculature). Thus, static 3D cultures do not accurately mimic in vivo cellular architecture and function. The development of a ‘dynamic’ culture environment has offered 3D culture models with the potential to improve the ‘naturalness’ of the cells being cultured and thereby have more in vivo relevance for translational research. This makes them relatively more superior than single cell-type static 3D cell cultures. Dynamic systems include magnetic- and acoustic-based assembly devices, micropocket cultures, dielectrophoretic and microfluidic platforms. Microfluidic devices might be the most versatile of these culture platforms, considering their engineering diversity, their potential to improve molecular crosstalk among culture elements and their prospective range of applications.

Keywords

In Vitro Applications, Scaffold-Based and Scaffold-Free Systems, Static and Dynamic Culture Systems, Three-dimensional Cell Culture.
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  • Dynamic Three-Dimensional Cell-Culture Systems for Enhanced in Vitro Applications

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Authors

Pargaonkar Aishwarya
Department of Human Genetics, Faculty of Biomedical Sciences, Technology and Research, Sri Ramachandra Institute of Higher Education and Research, Chennai 600 116, India
Gatika Agrawal
Department of Human Genetics, Faculty of Biomedical Sciences, Technology and Research, Sri Ramachandra Institute of Higher Education and Research, Chennai 600 116, India
Jennifer Sally
Department of Human Genetics, Faculty of Biomedical Sciences, Technology and Research, Sri Ramachandra Institute of Higher Education and Research, Chennai 600 116, India
Maddaly Ravi
Department of Human Genetics, Faculty of Biomedical Sciences, Technology and Research, Sri Ramachandra Institute of Higher Education and Research, Chennai 600 116, India

Abstract


The advent of dynamic three-dimensional cell cultures has transformed the field of biological research as they bridge the gap between in vitro and in vivo systems. 3D cell-culture techniques can be categorized into two types: static and dynamic culture systems. Traditional cell-culture models are considered to be ‘static’ in nature, as the cells are grown on matrices or scaffolds with little focus given to the complexity of the growth conditions that exist in the in vivo tissue microenvironments (presence of continuous blood supply for the development of tumour vasculature). Thus, static 3D cultures do not accurately mimic in vivo cellular architecture and function. The development of a ‘dynamic’ culture environment has offered 3D culture models with the potential to improve the ‘naturalness’ of the cells being cultured and thereby have more in vivo relevance for translational research. This makes them relatively more superior than single cell-type static 3D cell cultures. Dynamic systems include magnetic- and acoustic-based assembly devices, micropocket cultures, dielectrophoretic and microfluidic platforms. Microfluidic devices might be the most versatile of these culture platforms, considering their engineering diversity, their potential to improve molecular crosstalk among culture elements and their prospective range of applications.

Keywords


In Vitro Applications, Scaffold-Based and Scaffold-Free Systems, Static and Dynamic Culture Systems, Three-dimensional Cell Culture.

References





DOI: https://doi.org/10.18520/cs%2Fv122%2Fi2%2F149-160