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Potential of native forest leaves to modulate in vitro rumen fermentation and mitigate methane emission


Affiliations
1 ICAR-Eastern Regional Station, National Dairy Research Institute, Kalyani 741 235, India
2 ICAR-Central Sheep and Wool Research Institute, Avikanagar 304 501, India
 

Tree foliages rich in phytochemicals can be used as sustainable fodder for livestock to modulate rumen fermentation for cleaner and improved production. Samples of nine different forest tree leaves were collected from hilly regions of Arunachal Pradesh, India to study their effect on in vitro rumen fermentation and methane production. After 24 h of incubation, highest (P < 0.05) gas production (ml/g DM/24 h) was observed in Symplocos racemosa among the leaves. Methane production (ml/g DDM/24 h) was lowest (P < 0.05) in Symplocos crataegoides followed by Berberis aristata leaves, while in vitro true dry matter digestibility was highest (P < 0.05) for Berberis aristata leaves. In case of rumen fermentation attributes, B. aristata and S. crataegoides produced maximum volatile fatty acid and microbial biomass amongst other screened leaves. Therefore, these leaves can be used as a fodder supplement to address feed scarcity and reduce methanogenesis in ruminants of the North East hilly regions of India.

Keywords

Fodder supplement, hilly regions, methanogenesis, rumen fermentation, tree foliage.
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  • Malik, P. K., Kolte, A. P., Dhali, A., Sejian, V., Thirumalaisamy, G., Gupta, R. and Bhatta, R., GHG emissions from livestock: challenges and ameliorative measures to counter adversity. In Greenhouse Gases – Selected Case Studies (ed. Manning, A. J.), Rijeka, Croatia, 2016, pp. 1–16.

  • Patra, A. K., Min, B. R. and Saxena, J., Dietary tannins on microbial ecology of the gastrointestinal tract in ruminants. In Dietary Phytochemicals and Microbes, Vol. 237 (ed. Patra, A. K.), Springer, Dordrecht, The Netherlands, 2012, p. 62.

  • Bhatt, R. S., Sahoo, A., Sarkar, S., Saxena, V. K., Soni, L., Sharma, P. and Gadekar, Y. P., Dietary inclusion of nonconventional roughages for lowering enteric methane production and augmenting nutraceutical value of meat in cull sheep. Anim. Feed Sci. Technol., 2021, 273, 114832.

  • Choudhary, S., Santra, A., Sarkar, S. and Das, S. K., In vitro digestibility and fermentation kinetics of some north eastern Himalayan tree leaves using cattle rumen fluid as inoculum. Indian J. Anim. Sci., 2018, 88, 1085–1089.

  • Gupta, R., Dutta, T. K., Kundu, S. S., Chatterjee, A., Gautam, M. and Sarkar, S., Nutritional evaluation of tree leaves of Ayodhya hills of Purulia district, West Bengal. Indian J. Anim. Nutr., 2016, 33, 404–410.

  • Sarkar, S., Mohini, M., Mondal, G., Pandita, S., Nampoothiri, V. M. and Gautam, M., Effect of supplementing Aegle marmelos leaves on in vitro rumen fermentation and methanogenesis of diets varying in roughage to concentrate ratio. Indian J. Anim. Res., 2018, 52, 1180–1184.

  • Sarkar, S., Mohini. M., Sharma, A., Tariq, H. and Pal, R. P., Effect of supplementing Leucaena leucocephala leaves alone or in conjunction with malic acid on nutrient utilization, performance traits, and enteric methane emission in crossbred calves under tropical conditions. Trop. Anim. Health Prod., 2021, 53, 1–10.

  • Arango, J. et al., Ambition meets reality: achieving GHG emission reduction targets in the livestock sector of Latin America. Front. Sustain Food Syst., 2020, 4, 1–9; https://doi.org/10.3389/fsufs. 2020.00065

  • García-González, R., López, S., Fernández, M. and González, J. S., Effects of the addition of some medicinal plants on methane production in a rumen simulating fermenter (RUSITEC). In International Congress Series, Elsevier, 2006, vol. 1293, pp. 172–175.

  • Yusuf, A. O., Egbinola, O. O., Ekunseitan, D. A. and Salem, A. Z. M., Chemical characterization and in vitro methane production of selected agroforestry plants as dry season feeding of ruminants livestock. Agrofor. Syst., 2020, 94, 1481–1489.

  • National Research Council, Nutrient Requirements of Dairy Cattle, National Academy Press, Washington, DC, USA, 2001, 7th rev. edn.

  • Menke, K. H. and Steingass, H., Estimation of the energetic feed value obtained by chemical analysis and in vitro gas production using rumen fluid. Anim. Res. Dev., 1988, 28, 7–55.

  • Agarwal, N., Kamra, D. N., Chatterjee, P. N., Kumar, R. and Chaudhary, L. C., In vitro methanogenesis, microbial profile and fermentation of green forages with buffalo rumen liquoras influenced by 2-bromoethanesulphonic acid. Asian–Aust. J. Anim. Sci., 2008, 21, 818–823.

  • Barnett, A. J. G. and Reid, R. L., Studies on the production of volatile fatty acids from grass by rumen liquor in artificial rumen. The volatile fatty acid production from fresh grasses. J. Agric. Sci., 1957, 48, 315–321.

  • Cottyn, B. G. and Boucque, C. V., Rapid method for the gas-chromatographic determination of volatile fatty acids in rumen fluid. J. Agric. Food Chem., 1968, 16, 105–107.

  • Hristov, A. N., McAllister, T. A. and Cheng, K. J., Effect of diet, digesta processing, freezing and extraction procedure on some polysaccharide degrading activities of ruminal contents. Can. J. Anim. Sci., 1999, 79, 73–81.

  • Agarwal, N., Agarwal, I., Kamra, D. N. and Chaudhary, L. C., Diurnal variation in the activities of hydrolytic enzymes in different fractions of rumen contents of Murrah buffaloes. J. Appl. Anim. Res., 2000, 18, 73–80.

  • Miller, G. L., Use of dinitrosalicyclic acid reagent for determining reducing sugar. Anal. Chem., 1959, 31, 426–428.

  • Shewale, J. G. and Sadana, J. C., Cellulase and β-glucosidase production by a basidiomycete species. Can. J. Microbiol., 1978, 24, 1204–1216.

  • Blummel, M., Makkar, H. P. S. and Becker, K., In vitro gas production: a technique revisited. J. Anim. Physiol. Anim. Nutr., 1997, 77, 24–34.

  • Krishnamoorthy, U., Soller, H., Steingass, H. and Menke, K. H., Energy and protein evaluation of tropical feedstuffs for whole tract and ruminal digestion by chemical analyses and rumen inoculum studies in vitro. Anim. Feed Sci. Technol., 1995, 52, 177–188.

  • National Research Council, Nutrient Requirements of Dairy Cattle, National Academy Press, Washington, DC, USA, 1989, 6th rev. edn.

  • Hungate, R. E., The rumen protozoa. In The Rumen and its Microbes, Academic Press, New York, USA, 1966, pp. 92–147.

  • Kamra, D. N., Sawal, R. K., Pathak, N. N., Kewalramani, N. and Agarwal, N., Diurnal variation in ciliate protozoa in the rumen of black buck (Antilope cercicapra) fed green forage. Lett. Appl. Microbiol., 1991, 13, 165–167.

  • Molina-Botero, I. C. et al., Effect of the addition of Enterolobium cyclocarpum pods and Gliricidia sepium forage on dry matter degradation, volatile fatty acid concentration, and in vitro methane production. Trop. Anim. Health Prod., 2020; https://doi.org/10.1007/s11250-020-02324-4

  • Sahoo, A., Sarkar, S., Lal, B., Kumawat, P., Sharma, S. and De, K., Utilization of fruit and vegetable waste as an alternative feed resource for sustainable and eco-friendly sheep farming. Waste Manage., 2021, 128, 232–242.

  • Kamra, D. N., Pawar, M. and Singh, B., Effect of plant secondary metabolites on rumen methanogens and methane emission by ruminants. In Dietary Phytochemicals and Microbes (ed. Patra, A. K.), Springer, Dordrecht, The Netherlands, 2012, pp. 351–370.

  • Hundal, J. S., Wadhwa, M. and Bakshi, M. P. S., Effect of supplementing essential oils on the in vitro methane production and digestibility of wheat straw. J. Anim. Nutr., 2016, 1, 14; doi:10.21767/2572-5459.100014.

  • Sarkar, S., Mohini, M., Nampoothiri, V. M., Mondal, G. and Pandita, S., Effect of tree leaves and malic acid supplementation to wheat straw based substrates on in vitro rumen fermentation parameters. Indian J. Anim. Nutr., 2016, 33, 421–426.

  • Valencia-Salazar, S. S., Jiménez-Ferrer, G., Arango, J., MolinaBotero, I., Chirinda, N., Piñeiro-Vázquez, A. and Kú-Vera, J., Enterimethane mitigation and fermentation kinetics of forage species from southern Mexico: in vitro screening. Agrofor. Syst., 2021, 95, 293–305.

  • Bhatta, R., Saravanan, M., Baruah, L. and Prasad, C. S., Effects of graded levels of tannin-containing tropical tree leaves on in vitro rumen fermentation, total protozoa and methane production. J. Appl. Microbiol., 2015, 118, 557–564.

  • Lee, H. J., Lee, S. C., Kim, J. D., Oh, Y. G., Kim, B. K., Kim, C. W. and Kim, K. J., Methane production potential of feed ingredients measured by in vitro gas test. Asian–Aust. J. Anim. Sci., 2003, 16, 1143–1150.

  • Valenciaga, D., Chongo, B., Herrera, R. S., Torres, V., Oramas, A. and Herrera, M., Effect of regrowth age on in vitro dry matter digestibility of Pennisetum purpurecum cv. CUBACT-115. Cuban J. Agric. Sci., 2009, 43, 79–82.

  • Shingfield, K. J., Ahvenjarvi, S., Toivonen, V., Vanhatalo, A., Huhtanen, P. and Griinari, J. M., Effect of incremental levels of sunflower-seed oil in the diet on ruminal lipid metabolism in lactating cows. Br. J. Nutr., 2008, 99, 971–983.

  • Hess, H. D., Monosalve, L. M., Lascano, C. E., Carulla, J. E., Diaz, T. E. and Kreuzer, M., Supplementation of a tropical grass diet with forage legumes and Sapindus saponaria fruits: effect on in vitro ruminal nitrogen turnover and methanogenesis. Aust. J. Agric. Res., 2003, 54, 703–713.

  • Patra, A. K. and Saxena, J., Dietary phytochemicals as rumen modifiers: a review of the effects on microbial populations. Antone Van Leeuwenhoek, 2009, 96, 363–375.

  • Bhatta, R., Baruah, L., Saravanan, M., Suresh, K. P. and Sampath, K. T., Effect of medicinal and aromatic plants on rumen fermentation, protozoa population and methanogenesis in vitro. J. Anim. Physiol. Anim. Nutr., 2013, 97, 446–456.

  • Waghorn, G. C. and McNabb, W. C., Consequences of plant phenolic compounds for productivity and health of ruminants. Proc. Nutr. Soc., 2003, 62, 383–392.

  • Chaudhary, L. C., Srivastava, A. and Singh, K. K., Rumen fermentation pattern and digestion of structural carbohydrate in buffalo (Bubalus bubalis) calves as affected by ciliate protozoa. Anim. Feed Sci. Technol., 1995, 56, 111–117.

  • Ungerfeld, E. M., Shifts in metabolic hydrogen sinks in the methanogenesis-inhibited ruminal fermentation: a meta-analysis. Front. Microbiol., 2015, 6, 1–17.

  • Datt, C., Niranjan, M., Chhabra, A., Chatopadhyay, K. and Dhiman, T. R., Forage yield, chemical composition and in vitro digestibility of different cultivars of maize (Zea mays L.). Indian J. Dairy Sci., 2006, 59, 177–180.

  • Makkar, H. P. S., Sen, S., Blummel, M. and Becker, K., Effect of fractions containing saponins from Yucca schidigera, Quillaja saponaria and Acacia auriculofprmis on rumen fermentation. J. Agric. Food Chem., 1998, 46, 4324–4328


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  • Potential of native forest leaves to modulate in vitro rumen fermentation and mitigate methane emission

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Authors

Shilpa Choudhary
ICAR-Eastern Regional Station, National Dairy Research Institute, Kalyani 741 235, India
Ashok Santra
ICAR-Eastern Regional Station, National Dairy Research Institute, Kalyani 741 235, India
Srobana Sarkar
ICAR-Central Sheep and Wool Research Institute, Avikanagar 304 501, India
Ajoy Mandal
ICAR-Eastern Regional Station, National Dairy Research Institute, Kalyani 741 235, India
Subrata Kumar Das
ICAR-Eastern Regional Station, National Dairy Research Institute, Kalyani 741 235, India

Abstract


Tree foliages rich in phytochemicals can be used as sustainable fodder for livestock to modulate rumen fermentation for cleaner and improved production. Samples of nine different forest tree leaves were collected from hilly regions of Arunachal Pradesh, India to study their effect on in vitro rumen fermentation and methane production. After 24 h of incubation, highest (P < 0.05) gas production (ml/g DM/24 h) was observed in Symplocos racemosa among the leaves. Methane production (ml/g DDM/24 h) was lowest (P < 0.05) in Symplocos crataegoides followed by Berberis aristata leaves, while in vitro true dry matter digestibility was highest (P < 0.05) for Berberis aristata leaves. In case of rumen fermentation attributes, B. aristata and S. crataegoides produced maximum volatile fatty acid and microbial biomass amongst other screened leaves. Therefore, these leaves can be used as a fodder supplement to address feed scarcity and reduce methanogenesis in ruminants of the North East hilly regions of India.

Keywords


Fodder supplement, hilly regions, methanogenesis, rumen fermentation, tree foliage.

References





DOI: https://doi.org/10.18520/cs%2Fv122%2Fi3%2F322-327