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Efficient molecular method/s for detection of Bt brinjal Event 142


Affiliations
1 Division of Genomic Resources, ICAR-National Bureau of Plant Genetic Resources, New Delhi 110 012, India
 

Fruit and shoot borer (FSB)-resistant Bt brinjal Event 142 with cry1Fa1 gene has been approved for biosafety research level (BRL)-II field trials by the Genetic Engineering Appraisal Committee (GEAC), the competent authority in India. For regulatory compliance, systematic detection of genetically modified (GM) crops would facilitate moni­toring for the presence of a GM event in the supply chain. Polymerase chain reaction (PCR) and real-time PCR are widely used for GM detection globally. Qualitative PCR and real-time PCR assays targeting cry1Fa1 transgene were deve­loped and validated with acceptable specificity and sensitivity up to 0.01%. A multiplex (hexaplex) PCR-based screening assay simul­taneously detecting five transgenic elements and an endogenous gene was developed for Event 142. Construct-specific PCR reported herein could be emplo­yed for more specific detection. These detection assays would facilitate efficient regulatory compliance by checking unapproved Bt brinjal event in the supply chain and also address the concerns of the consumers to an extent.

Keywords

Bt brinjal, detection assays, field trials, genetically modified crops, polymerase chain reaction.
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  • Efficient molecular method/s for detection of Bt brinjal Event 142

Abstract Views: 196  |  PDF Views: 104

Authors

Ruchi Sharma
Division of Genomic Resources, ICAR-National Bureau of Plant Genetic Resources, New Delhi 110 012, India
Monika Singh
Division of Genomic Resources, ICAR-National Bureau of Plant Genetic Resources, New Delhi 110 012, India
Gurinderjit Randhawa
Division of Genomic Resources, ICAR-National Bureau of Plant Genetic Resources, New Delhi 110 012, India

Abstract


Fruit and shoot borer (FSB)-resistant Bt brinjal Event 142 with cry1Fa1 gene has been approved for biosafety research level (BRL)-II field trials by the Genetic Engineering Appraisal Committee (GEAC), the competent authority in India. For regulatory compliance, systematic detection of genetically modified (GM) crops would facilitate moni­toring for the presence of a GM event in the supply chain. Polymerase chain reaction (PCR) and real-time PCR are widely used for GM detection globally. Qualitative PCR and real-time PCR assays targeting cry1Fa1 transgene were deve­loped and validated with acceptable specificity and sensitivity up to 0.01%. A multiplex (hexaplex) PCR-based screening assay simul­taneously detecting five transgenic elements and an endogenous gene was developed for Event 142. Construct-specific PCR reported herein could be emplo­yed for more specific detection. These detection assays would facilitate efficient regulatory compliance by checking unapproved Bt brinjal event in the supply chain and also address the concerns of the consumers to an extent.

Keywords


Bt brinjal, detection assays, field trials, genetically modified crops, polymerase chain reaction.

References





DOI: https://doi.org/10.18520/cs%2Fv122%2Fi4%2F464-468