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Significance of altered glycosyltransferase expression levels in oral cancer


Affiliations
1 SDM Research Institute for Biomedical Sciences, A Constituent Unit of Shri Dharmasthala Manjunatheshwara University, 5th Floor, Manjushree Building, Sattur, Dharwad 580 009, Department of Biotechnology, Basaveshwar Engineering College, Vidyagiri, Bagalkot 587 102, India
2 SDM Craniofacial Surgery and Research Centre, A Constituent unit of Shri Dharmasthala Manjunatheshwara University, Sattur, Dharwad 580 009, India
3 Department of Oral Pathology, SDM College of Dental Sciences and Hospital, A Constituent Unit of Shri Dharmasthala Manjunatheshwara University, Sattur, Dharwad 580 009, India
4 SDM Research Institute for Biomedical Sciences, A Constituent Unit of Shri Dharmasthala Manjunatheshwara University, 5th Floor, Manjushree Building, Sattur, Dharwad 580 009, India
5 Department of Microbiology, Davangere University, Shivagangotri, Davangere 577 002, India
 

We evaluate the relative quantification of mRNA expression levels of the gene encoding an enzyme that catal­yses initial step in glycosylation of dolichol-linked oligosaccharide such as dolichyl-phosphate N-acetyl­glucosaminephosphotransferase 1 (DPAGT1). Further­more, beta-1,4-mannosyl-glycoprotein 4-beta-N-acetyl­glucosaminyltransferase (MGAT3) and alpha-1,6-man­nosylglycoprotein 6-beta-N-acetylglucosaminyltransferase (MGAT5) mediated N-glycosylation, selected a2, 3, a2, 6 sialyltransferases families and sialidase by using the Real-Time Quantitative Reverse Transcription PCR (qRT-PCR). Thirty human oral cancer tissue samples and twelve control samples were examined. Results indi­cated significant upregulation of ST6 N-acetylgalactos­aminide alpha-2,6-sialyltransferase 1 (ST6GALNAC1) in oral cancer compared to controls. Additionally, the expressions of DPAGT1, MGAT3, MGAT5, ST3 beta-galactoside alpha-2,3-sialyltransferase 1 (ST3GAL1), ST6 beta-galactoside alpha-2,6-sialyltransferase 1 (ST6­GAL1), and ST6GALNAC1 were considerably higher in the tumour in tongue lesion compared to the buccal cavity. Hence, our experiment demonstrates significance of glycosyltransferases in the progression of oral cancer

Keywords

Aberrant Glycosylation, Invasion, Metastasis, Oral Cancer, Sialyltransferase
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  • Significance of altered glycosyltransferase expression levels in oral cancer

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Authors

Mallikarjun Goni
SDM Research Institute for Biomedical Sciences, A Constituent Unit of Shri Dharmasthala Manjunatheshwara University, 5th Floor, Manjushree Building, Sattur, Dharwad 580 009, Department of Biotechnology, Basaveshwar Engineering College, Vidyagiri, Bagalkot 587 102, India
Anil Kumar Desai
SDM Craniofacial Surgery and Research Centre, A Constituent unit of Shri Dharmasthala Manjunatheshwara University, Sattur, Dharwad 580 009, India
Niranjan Kumar
SDM Craniofacial Surgery and Research Centre, A Constituent unit of Shri Dharmasthala Manjunatheshwara University, Sattur, Dharwad 580 009, India
Veda Hegde
Department of Oral Pathology, SDM College of Dental Sciences and Hospital, A Constituent Unit of Shri Dharmasthala Manjunatheshwara University, Sattur, Dharwad 580 009, India
Palaksha Kanive Javaregowda
SDM Research Institute for Biomedical Sciences, A Constituent Unit of Shri Dharmasthala Manjunatheshwara University, 5th Floor, Manjushree Building, Sattur, Dharwad 580 009, India
D. B. M. Virupakshaiah
Department of Microbiology, Davangere University, Shivagangotri, Davangere 577 002, India

Abstract


We evaluate the relative quantification of mRNA expression levels of the gene encoding an enzyme that catal­yses initial step in glycosylation of dolichol-linked oligosaccharide such as dolichyl-phosphate N-acetyl­glucosaminephosphotransferase 1 (DPAGT1). Further­more, beta-1,4-mannosyl-glycoprotein 4-beta-N-acetyl­glucosaminyltransferase (MGAT3) and alpha-1,6-man­nosylglycoprotein 6-beta-N-acetylglucosaminyltransferase (MGAT5) mediated N-glycosylation, selected a2, 3, a2, 6 sialyltransferases families and sialidase by using the Real-Time Quantitative Reverse Transcription PCR (qRT-PCR). Thirty human oral cancer tissue samples and twelve control samples were examined. Results indi­cated significant upregulation of ST6 N-acetylgalactos­aminide alpha-2,6-sialyltransferase 1 (ST6GALNAC1) in oral cancer compared to controls. Additionally, the expressions of DPAGT1, MGAT3, MGAT5, ST3 beta-galactoside alpha-2,3-sialyltransferase 1 (ST3GAL1), ST6 beta-galactoside alpha-2,6-sialyltransferase 1 (ST6­GAL1), and ST6GALNAC1 were considerably higher in the tumour in tongue lesion compared to the buccal cavity. Hence, our experiment demonstrates significance of glycosyltransferases in the progression of oral cancer

Keywords


Aberrant Glycosylation, Invasion, Metastasis, Oral Cancer, Sialyltransferase

References





DOI: https://doi.org/10.18520/cs%2Fv123%2Fi1%2F52-58