Current Science

Open Access Open Access  Restricted Access Subscription Access

Physiological, biochemical and molecular manifestations in response to seed priming with elicitors under drought in cotton


Affiliations
1 ICAR-Central Institute for Cotton Research, Nagpur 441 108, India; Sam Higginbottom University of Agriculture, Technology and Sciences, Allahabad 221 007, India
2 ICAR-Central Institute for Cotton Research, Nagpur 441 108, India
3 Sam Higginbottom University of Agriculture, Technology and Sciences, Allahabad 221 007, India
 

Water stress has a detrimental effect on growth and development, which alters physio-biochemical activities. Seed priming with elicitors such as methyl jasmonate and paclobutrazol can mitigate the impact of drought stress. Therefore, pot-culture studies were conducted with drought-tolerant (DTS-155) and drought-suscep­tible (IC-357055) cotton genotypes to assess the seed priming effects of elicitors (methyl jasmonate and paclobutrazol) on the physio-biochemical changes and gene expression. The dose (50, 100, 150 and 200 mM) and time interval (1.5 and 2.5 h) experiments of both the elicitors were performed separately. On the basis of germination, seedling growth and vigour, a 150 mM elicitor for 1.5 h time interval was found to be the best. Biochemical and physiological parameters confirmed an increase in relative water content, total antioxidant activities, chlorophyll, superoxide dismutase, catalase and proline under drought stress in both the genotypes, but a decrease in lipid peroxidation. Among the elicitors, methyl jasmonate improved drought tolerance as compared to paclobutrazol. Gene expression studies with Rub-S, Rub-L and Osmotin confirmed the results. Transcript abundance of Osmotin and Rub-L was upregulated under drought stress in both the genotypes and was highest in methyl jasmonate primed samples. These findings suggest that priming with methyl jasmonate enhances drought tolerance in cotton

Keywords

Drought responsive gene, Gossypium hirsu-tum, methyl jasmonate, paclobutrazol, seed priming
User
Notifications
Font Size

  • Baffes, J., Tanzania’s cotton sector: reforms, constraints and chal-lenges. Dev. Policy Rev., 2005, 22(1), 75–96.

  • Gillham, F. E., Cotton Production Prospects for the Next Decade, World Bank Publications, 1995, vol. 23.

  • Li, X., Cai, J., Liu, F., Dai, T., Cao, W. and Jiang, D., Cold priming drives the sub-cellular antioxidant systems to protect photosynthetic electron transport against subsequent low temperature stress in win-ter wheat. Plant Physiol. Biochem., 2014, 82, 34–43.

  • Muscolo, A., Junker, A., Klukas, C., Weigelt-Fischer, K., Riewe, D. and Altmann, T., Phenotypic and metabolic responses to drought and salinity of four contrasting lentil accessions. J. Exp. Bot., 2015, 66(18), 5467–5480.

  • Paparella, S., Araújo, S. S., Rossi, G., Wijayasinghe, M., Carbonera, D. and Balestrazzi, A., Seed priming: state of the art and new pers-pectives. Plant Cell Rep., 2015, 34(8), 1281–1293.

  • Hussain, S., Yin, H., Peng, S., Khan, F. A., Khan, F., Sameeullah, M. and Nie, L., Comparative transcriptional profiling of primed and non-primed rice seedlings under submergence stress. Front. Plant Sci., 2016, 7, 1125.

  • Bandurska, S. A., Tanveer, M., Hussain, S., Tung, S. A., Samad, R. A., Wang, L. and Shahzad, B., Exogenously applied methyl jasmonate improves the drought tolerance in wheat imposed at early and late developmental stages. Acta Physiol. Plant., 2003, 38(1), 25.

  • Barrs, H. D. and Weatherley, P. E., A re-examination of the relative turgidity technique for estimating water deficits in leaves. Aust. J. Biol. Sci., 1962, 15(3), 413–428.

  • Heath, R. L. and Packer, L., Photoperoxidation in isolated chloro-plasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Arch. Biochem. Biophys., 1968, 125, 189–198.

  • Bates, L. S., Waldren, R. P. and Teare, I. D., Rapid determination of free proline for water-stress studies. Plant Soil, 1973, 39(1), 205–207.

  • Chance, B. and Maehly, A. C., Assay of catalases and peroxidases. Methods Enzymol., 1955, 2, 764–775.

  • Salome, 2016; http://www.chem.ucla.edu/dept/Faculty/merchant/ pdf/SOD%20gels%20protocol%2011-2016.pdf 13. Bradford, M. M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 1976, 72(1–2), 248–254.

  • Lobell, D. B., Schlenker, W. and Costa-Roberts, J., Climate trends and global crop production since 1980. Science, 2011, 333(6042), 616–620.

  • Ullah, A., Sun, H., Yang, X. and Zhang, X., Drought coping strate-gies in cotton: increased crop per drop. Plant Biotechnol. J., 2017, 15(3), 271–284.

  • Munné-Bosch, S. and Alegre, L., Changes in carotenoids, tocophe-rols and diterpenes during drought and recovery, and the biological significance of chlorophyll loss in Rosmarinus officinalis plants. Planta, 2000, 210(6), 925–931.

  • Moller, I. M., Jensen, P. E. and Hansson, A., Oxidative modifica-tions to cellular components in plants. Annu. Rev. Plant Biol., 2007, 58, 459–481.

  • Moran, J. F., Becana, M., Iturbe-Ormaetxe, I., Frechilla, S., Klucas, R. V. and Aparicio-Tejo, P., Drought induces oxidative stress in pea plants. Planta, 1994, 194, 346–352.

  • McMichael, B. L. and Elmore, C. D., Proline accumulation in water stressed cotton leaves. Crop Sci., 1977, 17(6), 905–908.

  • Ratnayaka, H. H., Molin, W. T. and Sterling, T. M., Physiological and antioxidant responses of cotton and spurred anoda under inter-ference and mild drought. J. Exp. Bot., 2003, 54(391), 2293–2305.

  • Egert, M. and Tevini, M., Influence of drought on some physiologi-cal parameters symptomatic for oxidative stress in leaves of chives (Allium schoenoprasum). Environ. Exp. Bot., 2002, 48, 43–49.

  • Wang, Z. and Huang, B., Physiological recovery of Kentucky blue-grass from simultaneous drought and heat stress. Crop Sci., 2004, 44, 1729–1736.

  • Huseynova, I. M., Aliyeva, D. R. and Aliyev, J. A., Subcellular loca-lization and responses of superoxide dismutase isoforms in local wheat varieties subjected to continuous soil drought. Plant Physiol. Biochem., 2014, 81, 54–60.

  • Somasundaram, R., Jaleel, C. A., Abraham, S. S., Azooz, M. M. and Panneerselvam, R., Role of paclobutrazol and ABA in drought stress amelioration in Sesamum indicum L. Global J. Mol. Sci., 2009, 4(2), 56–62.

  • Parkhi, V., Kumar, V., Sunilkumar, G., Campbell, L. M., Singh, N. K. and Rathore, K. S., Expression of apoplastically secreted tobacco osmotin in cotton confers drought tolerance. Mol. Breed., 2009, 23(4), 625–639.

  • Singh, N. K., Handa, A. K., Hasegawa, P. M. and Bressan, R. A., Proteins associated with adaptation of cultured tobacco cells to NaCl. Plant Physiol., 1985, 79(1), 126–137.

  • Law, R. D. and Crafts-Brandner, S. J., High temperature stress increases the expression of wheat leaf ribulose-1,5-bisphosphate carboxylase/oxygenase activase protein. Arch. Biochem. Biophys., 2001, 386(2), 261–267.

  • Pfaffl, M. W., A new mathematical model for relative quantifica-tion in real-time RT-PCR. Nucl. Acid Res., 2001, 29(9), 45.


Abstract Views: 379

PDF Views: 119




  • Physiological, biochemical and molecular manifestations in response to seed priming with elicitors under drought in cotton

Abstract Views: 379  |  PDF Views: 119

Authors

Himanshu Kumar
ICAR-Central Institute for Cotton Research, Nagpur 441 108, India; Sam Higginbottom University of Agriculture, Technology and Sciences, Allahabad 221 007, India
Pooja Verma
ICAR-Central Institute for Cotton Research, Nagpur 441 108, India
Suchit A. John
Sam Higginbottom University of Agriculture, Technology and Sciences, Allahabad 221 007, India
D. Blaise
ICAR-Central Institute for Cotton Research, Nagpur 441 108, India

Abstract


Water stress has a detrimental effect on growth and development, which alters physio-biochemical activities. Seed priming with elicitors such as methyl jasmonate and paclobutrazol can mitigate the impact of drought stress. Therefore, pot-culture studies were conducted with drought-tolerant (DTS-155) and drought-suscep­tible (IC-357055) cotton genotypes to assess the seed priming effects of elicitors (methyl jasmonate and paclobutrazol) on the physio-biochemical changes and gene expression. The dose (50, 100, 150 and 200 mM) and time interval (1.5 and 2.5 h) experiments of both the elicitors were performed separately. On the basis of germination, seedling growth and vigour, a 150 mM elicitor for 1.5 h time interval was found to be the best. Biochemical and physiological parameters confirmed an increase in relative water content, total antioxidant activities, chlorophyll, superoxide dismutase, catalase and proline under drought stress in both the genotypes, but a decrease in lipid peroxidation. Among the elicitors, methyl jasmonate improved drought tolerance as compared to paclobutrazol. Gene expression studies with Rub-S, Rub-L and Osmotin confirmed the results. Transcript abundance of Osmotin and Rub-L was upregulated under drought stress in both the genotypes and was highest in methyl jasmonate primed samples. These findings suggest that priming with methyl jasmonate enhances drought tolerance in cotton

Keywords


Drought responsive gene, Gossypium hirsu-tum, methyl jasmonate, paclobutrazol, seed priming

References





DOI: https://doi.org/10.18520/cs%2Fv123%2Fi5%2F658-666