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Surveillance of SARS-CoV-2 genome fragment in urban, peri-urban and rural water bodies: a temporal and comparative analysis


Affiliations
1 Bioengineering and Environmental Sciences Laboratory, Department of Energy and Environmental Engineering, CSIR-Indian Institute of Chemical Technology, Hyderabad 500 007, India; Academy of Scientific and Innovative Research, Ghaziabad 201 002, India, India
2 Academy of Scientific and Innovative Research, Ghaziabad 201 002, India; CSIR-Centre for Cellular and Molecular Biology, Hyderabad 500 007, India, India
3 CSIR-Centre for Cellular and Molecular Biology, Hyderabad 500 007, India, India
4 CSIR-Centre for Cellular and Molecular Biology, Hyderabad 500 007, India; Tata Institute of Genetics and Society, Centre at inStem, NCBS Campus, Bengaluru 560 065, India, India
 

As a result of the SARS-CoV-2 pandemic, water bodies connected to anthropogenic activities may likely reveal the presence of viral genetic material. Urban, peri-urban and rural water bodies in and around Hyderabad, Telangana, India, were monitored for the presence of SARS-CoV-2 gene fragments during the first and second wave of COVID-19 infection. The SARS-CoV-2 genes were not detected in peri-urban and rural lakes, whereas urban lakes having direct functional attributes from domestic activity showed prevalence. Distinct variability in viral load observed among five water bodies was in concordance with human activity in the catchment area. High viral load was observed during the peaks of the first and second waves, specifically in urban lakes
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  • Surveillance of SARS-CoV-2 genome fragment in urban, peri-urban and rural water bodies: a temporal and comparative analysis

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Authors

Manupati Hemalatha
Bioengineering and Environmental Sciences Laboratory, Department of Energy and Environmental Engineering, CSIR-Indian Institute of Chemical Technology, Hyderabad 500 007, India; Academy of Scientific and Innovative Research, Ghaziabad 201 002, India, India
Athmakuri Tharak
Bioengineering and Environmental Sciences Laboratory, Department of Energy and Environmental Engineering, CSIR-Indian Institute of Chemical Technology, Hyderabad 500 007, India; Academy of Scientific and Innovative Research, Ghaziabad 201 002, India, India
Harishankar Kopperi
Bioengineering and Environmental Sciences Laboratory, Department of Energy and Environmental Engineering, CSIR-Indian Institute of Chemical Technology, Hyderabad 500 007, India; Academy of Scientific and Innovative Research, Ghaziabad 201 002, India, India
Uday Kiran
Academy of Scientific and Innovative Research, Ghaziabad 201 002, India; CSIR-Centre for Cellular and Molecular Biology, Hyderabad 500 007, India, India
C. G. Gokulan
CSIR-Centre for Cellular and Molecular Biology, Hyderabad 500 007, India, India
Rakesh K. Mishra
CSIR-Centre for Cellular and Molecular Biology, Hyderabad 500 007, India; Tata Institute of Genetics and Society, Centre at inStem, NCBS Campus, Bengaluru 560 065, India, India
S. Venkata Mohan
Bioengineering and Environmental Sciences Laboratory, Department of Energy and Environmental Engineering, CSIR-Indian Institute of Chemical Technology, Hyderabad 500 007, India; Academy of Scientific and Innovative Research, Ghaziabad 201 002, India, India

Abstract


As a result of the SARS-CoV-2 pandemic, water bodies connected to anthropogenic activities may likely reveal the presence of viral genetic material. Urban, peri-urban and rural water bodies in and around Hyderabad, Telangana, India, were monitored for the presence of SARS-CoV-2 gene fragments during the first and second wave of COVID-19 infection. The SARS-CoV-2 genes were not detected in peri-urban and rural lakes, whereas urban lakes having direct functional attributes from domestic activity showed prevalence. Distinct variability in viral load observed among five water bodies was in concordance with human activity in the catchment area. High viral load was observed during the peaks of the first and second waves, specifically in urban lakes

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DOI: https://doi.org/10.18520/cs%2Fv123%2Fi8%2F987-994