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Cross-resistance and biochemical mechanism in an insecticide-resistant population of Trichogramma chilonis Ishii (Hymenoptera: Trichogrammatidae) and its parasitizing efficiency against invasive fall armyworm Spodoptera frugiperda (J.E. Smith)


Affiliations
1 Department of Entomology, University of Agricultural Sciences, Gandhi Krishi Vignan Kendra, Bellary Road, Bengaluru 560 065, India, India
2 ICAR-National Bureau of Agricultural Insect Resources, Hebbal, Bengaluru 560 024, India, India
3 ICAR-National Bureau of Agricultural Insect Resources, Hebbal, Bengaluru 560 024, India, India
 

Trichogramma chilonis is an egg parasitoid of lepidopteran pests and widely used in biological control and integrated pest management (IPM) programmes. In this study, the cross-resistance in multiple insecticide-tolerant strain of T. chilonis was evaluated against chlorantraniliprole 18.5% SC, spinetoram 11.7% SC and thiamethoxam 12.6% + lambda-cyhalothrin 9.5% ZC. Bioassay studies revealed the highest resistance level against chlorantraniliprole (8.83-fold resistance) over the susceptible population, followed by spinetoram (2.41-fold). Metabolic enzymes carboxylesterase and glutathione S-transferase showed major involvement in the resistant populations, with the highest activity obser­ved against chlorantraniliprole, followed by spinetoram. The resistant population at field recommended doses of chlorantraniliprole (400 ppm), parasitized 52.92% and 44.10% of Corcyra cephalonica and Spodoptera frugiperda eggs respectively, compared to 15.48% and 9.6% parasitism by the susceptible population. Integration and utilization of resistant strains of T. chilonis in IPM programmes can provide improved control of insect pests under insecticide-sprayed conditions and may reduce the insecticide load on crops
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  • Cross-resistance and biochemical mechanism in an insecticide-resistant population of Trichogramma chilonis Ishii (Hymenoptera: Trichogrammatidae) and its parasitizing efficiency against invasive fall armyworm Spodoptera frugiperda (J.E. Smith)

Abstract Views: 157  |  PDF Views: 97

Authors

Priyanka Dupatne
Department of Entomology, University of Agricultural Sciences, Gandhi Krishi Vignan Kendra, Bellary Road, Bengaluru 560 065, India, India
T. Venkatesan
ICAR-National Bureau of Agricultural Insect Resources, Hebbal, Bengaluru 560 024, India, India
Omprakash Navik
ICAR-National Bureau of Agricultural Insect Resources, Hebbal, Bengaluru 560 024, India, India
M. Mohan
ICAR-National Bureau of Agricultural Insect Resources, Hebbal, Bengaluru 560 024, India, India
K. M. Venugopal
ICAR-National Bureau of Agricultural Insect Resources, Hebbal, Bengaluru 560 024, India, India
Basavaarya
ICAR-National Bureau of Agricultural Insect Resources, Hebbal, Bengaluru 560 024, India, India
V. Linga
ICAR-National Bureau of Agricultural Insect Resources, Hebbal, Bengaluru 560 024, India, India
Y. Lalitha
ICAR-National Bureau of Agricultural Insect Resources, Hebbal, Bengaluru 560 024, India, India
G. Sivakumar
ICAR-National Bureau of Agricultural Insect Resources, Hebbal, Bengaluru 560 024, India, India
M. Ashwini
Department of Entomology, University of Agricultural Sciences, Gandhi Krishi Vignan Kendra, Bellary Road, Bengaluru 560 065, India, India

Abstract


Trichogramma chilonis is an egg parasitoid of lepidopteran pests and widely used in biological control and integrated pest management (IPM) programmes. In this study, the cross-resistance in multiple insecticide-tolerant strain of T. chilonis was evaluated against chlorantraniliprole 18.5% SC, spinetoram 11.7% SC and thiamethoxam 12.6% + lambda-cyhalothrin 9.5% ZC. Bioassay studies revealed the highest resistance level against chlorantraniliprole (8.83-fold resistance) over the susceptible population, followed by spinetoram (2.41-fold). Metabolic enzymes carboxylesterase and glutathione S-transferase showed major involvement in the resistant populations, with the highest activity obser­ved against chlorantraniliprole, followed by spinetoram. The resistant population at field recommended doses of chlorantraniliprole (400 ppm), parasitized 52.92% and 44.10% of Corcyra cephalonica and Spodoptera frugiperda eggs respectively, compared to 15.48% and 9.6% parasitism by the susceptible population. Integration and utilization of resistant strains of T. chilonis in IPM programmes can provide improved control of insect pests under insecticide-sprayed conditions and may reduce the insecticide load on crops

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DOI: https://doi.org/10.18520/cs%2Fv124%2Fi1%2F115-122