Current Science

Open Access Open Access  Restricted Access Subscription Access

Chemical Constituents of Essential Oil from Leaves of an Invasive Weed Ageratina adenophora in Central Nepal


Affiliations
1 Department of Chemistry, Trichandra Campus, Tribhuvan University, Kathmandu, Nepal
2 Central Department of Botany, Institute of Science and Technology, Tribhuvan University, Nepal
3 Department of Food Technology and Quality Control, Government of Nepal, Kathmandu, Nepal
4 Department of Forest Resources, Ministry of Forest and Environment, Plant Research Center, Jumla, Nepal
5 Department of Biology, Kailali Multiple Campus, Far Western University, Kailali, Nepal
6 Central Department of Environmental Science, Institute of Science and Technology, Tribhuvan University, Nepal
7 Biological Resource Unit, Nepal Academy of Science and Technology, Lalitpur, Nepal
 

This study aims to identify volatile chemical components in the essential oil of an invasive plant, Ageratina adenophora, from Central Nepal. Leaf samples of A. adenophora were collected, and the chemical composition of essential oil was analysed by gas chromatography (GC) and GC–mass spectrometry (GC/MS). A total of 27 components in the oil were identified. The major compounds were cadinol, bisabolol and bornyl acetate. The amount of valencene, elemol and 2(1H)-naphthalenone, 4a,5,6,7,8,8a-hexahydro-6-[1-(hydroxymethyl)ethenyl]-4,8a-dimethyl-, [4ar(4a.alpha.,6.alpha.,8a.beta.]- varied with samples collected from different elevations. Five compounds, viz. cloven, β-gurjuene, β-cubebene, verbenone and cis-β-farnesene were rarely reported previously from A. adenophora. Being an invasive and medicinal plant, A. adenophora is found in varied ecological conditions. Further studies on the variation in amount and composition, phytotoxicity and benefits of such compounds are recommended.

Keywords

Ageratina adenophora, Chemical Constituents, Essential Oil, Gas Chromatography, Mass Spectrometry.
User
Notifications
Font Size

  • Muniappan, R., Raman, A. and Redd, G. V. (eds), Ageratina adenophora (Sprengel) King and Robinson (Asteraceae). In Biological Control of Tropical Weeds using Arthropods, Cambridge University Press, Cambridge, UK, 2009, pp. 1–16.

  • Yang, G. Q., Qiu, W. R., Jin, Y. N. and Wan, F. H., Potential allelo-chemicals from root exudates of invasive Ageratina adenophora. Allelopathy J., 2013, 32, 233.

  • Tiwari, S., Siwakoti, M., Adhikari, B. and Subedi, K., An inventory and assessment of invasive alien plant species of Nepal. IUCN – The World Conservation Union, Nepal, 2005, p. 114.

  • Thapa, L. B., Thapa, H. and Magar, B. G., Perception, trends and impacts of climate change in Kailali District, Far West Nepal. Int. J. Environ., 2015, 4, 62–76.

  • Thapa, L. B., Kaewchumnong, K., Sinkkonen, A. and Sridith, K., Plant communities and Ageratina adenophora invasion in lower montane vegetation, Central Nepal. Int. J. Ecol. Dev., 2016, 31, 35–49.

  • Tripathi, R. S., Yadav, A. S. and Kushwaha, S. P. S., Biology of Chromolaena odorata, Ageratina adenophora and Ageratina riperia: a review. In Invasive Alien Plants: An Ecological Appraisal for the Indian Subcontinent (eds Bhatt, J. R. et al.), CAB International, Cambridge, UK, 2012, pp. 3–56.

  • Zhao, X., Zheng, G. W., Niu, X. M., Li, W. Q., Wang, F. S. and Li, S. H., Terpenes from Eupatorium adenophorum and their allelopathic effects on Arabidopsis seeds germination. J. Agric. Food Chem., 2009, 57, 478–482.

  • Lallianrawna, S., Muthukumaran, R., Ralte, V., Gurusubramanian, G. and Kumar, N. S., Determination of total phenolic content, total flavonoid content and total antioxidant capacity of Ageratina adenophora (Spreng.) King & H. Rob. Sci. Vis., 2013, 13, 149–156.

  • Tererai, F. and Wood, A. R., On the present and potential distribution of Ageratina adenophora (Asteraceae) in South Africa. S. Afr. J. Bot., 2014, 95, 152–158.

  • Thapa, L. B., Kaewchumnong, K., Sinkkonen, A. and Sridith, K., ‘Soaked in rainwater’ effect of Ageratina adenophora on seedling growth and development of native tree species in Nepal. Flora, 2020, 263, 151554.

  • Niu, H. B., Liu, W. X., Wan, F. H. and Liu, B., An invasive aster (Ageratina adenophora) invades and dominates forest understories in China: altered soil microbial communities facilitate the invader and inhibit natives. Plant Soil, 2007, 294, 73–85.

  • Xu, C. W. et al., Changes in non-symbiotic nitrogen-fixing bacteria inhabiting rhizosphere soils of an invasive plant Ageratina adenophora. Appl. Soil Ecol., 2012, 54, 32–38.

  • Bajpai, D. and Inderjit, I., Impact of nitrogen availability and soil communities on biomass accumulation of an invasive species. AoB Plants, 2013, 5, plt045.

  • Kundu, A., Saha, S., Ahluwalia, V. and Walia, S., Plant growth inhibitory terpenes from Eupatorium adenophorum leaves. J. Appl. Bot. Food Qual., 2013, 86, 33–36.

  • Wan, H., Liu, W. and Wan, F., Allelopathic effect of Ageratina adenophora (Spreng.) leaf litter on four herbaceous plants in invaded regions. J. Eco-Agric., 2011, 19, 130–134.

  • Thapa, L. B., Deuba, R., Oli, N., Singh, U. K. and Jha, S. K., Invasion status of alien weeds in the historical Chobhar area of Kathmandu valley, Nepal. Indian J. Weed Sci., 2020, 52, 405–407.

  • Thapa, L. B., Kaewchumnong, K., Sinkkonen, A. and Sridith, K., Plant invasiveness and target plant density: high densities of native Schima wallichii seedlings reduce negative effects of invasive Ageratina adenophora. Weed Res., 2017, 57, 72–80.

  • Thapa, L. B., Kaewchumnong, K., Sinkkonen, A. and Sridith, K., Airborne and belowground phytotoxicity of invasive Ageratina adenophora on native species in Nepal. Plant Ecol., 2020, 221, 883–892.

  • Shrestha, B. B., Invasive alien plant species in Nepal. In Frontiers of Botany (eds Jha, P. K., Siwakoti, M. and Rajbhandari, S.), Central Department of Botany, Tribhuvan University, Kathmandu, Nepal, 2016, pp. 269–284.

  • Darji, T. B. et al., Phytotoxic effects of invasive Ageratina adenophora on two native subtropical shrubs in Nepal. Sci. Rep., 2021, 11, 1–9.

  • Torres-Barajas, L., Rojas-Vera, J., Morales-Mendez, A., Rojas-Fermin, L., Lucena, M. and Buitrago, A., Chemical composition and evaluation of antibacterial activity of essential oils of Ageratina jahnii and Ageratina pichinchensis collected in Mérida, Venezuela. Biol. Latinoam. Caribe Plantas Med. Aromát., 2013, 12, 92–98.

  • Rajeswary, M., Govindarajan, M., Murugan, K., Hwang, J. S., Barnard, D. R., Amsath, A. and Muthukumaran, U., Ovicidal efficacy of Ageratina adenophora (family: Asteraceae) against Anopheles stephensi (Diptera: Culicidae). Int. J. Pure Appl. Zool., 2014, 2, 196–199.

  • Zhang, M. et al., Bioactive quinic acid derivatives from Ageratina adenophora. Molecules, 2013, 18, 14096–14104.

  • Padalia, R. C., Bisht, D. S., Joshi, S. C. and Mathela, C. S., Chemical composition of the essential oil from Eupatorium adenophorum Spreng. J. Essent. Oil Res., 2009, 21, 522–524.

  • Pala-Paul, J., Perez-Alonso, M. J., Velasco-Negueruela, A. and Sanz, J., Analysis by gas chromatography–mass spectrometry of the volatile components of Ageratina adenophora Spreng., growing in the Canary Islands. J. Chromatogr. A, 2002, 947, 327–331.

  • Subba, B. and Kandel, R. C., Chemical composition and bioactivity of essential oil of Ageratina adenophora from Bhaktapur district of Nepal. J. Nepal Chem. Soc., 2012, 30, 78–86.

  • Samuel, L., Muthukumaran, R. B., Gurusubramanian, G. and Senthilkumar, N., Larvicidal activity of Ipomoea cairica (L.) Sweet and Ageratina adenophora (Spreng.) King & H. Rob. plant extracts against arboviral and filarial vector, Culex quinquefasciatus Say (Diptera: Culicidae). Exp. Parasitol., 2014, 141, 112–121.

  • Weyerstahl, P., Marschall, H., Seelmann, I. and Kaul, V. K., Constituents of the flower essential oil of Ageratina adenophora (Spreng.) K. et R. from India. Flavour. Fragr. J., 1997, 12, 387–396.

  • Giri, S., Sahu, R., Paul, P., Nandi, G. and Dua, T. K., An updated review on Eupatorium adenophorum Spreng. [Ageratina adenophora (Spreng.)]: traditional uses, phytochemistry, pharmacological activities and toxicity. Pharmacol. Res.-Mod. Chin. Med., 2022, 100068.

  • Pandey, A. K., Mohan, M., Singh, P., Palni, U. T. and Tripathi, N. N., Chemical composition, antibacterial and antioxidant activity of essential oil of Eupatorium adenophorum Spreng. from eastern Uttar Pradesh, India. Food Biosci., 2014, 7, 80–87.

  • Talebi, S. M., Nohooji, M. G., Yarmohammadi, M., Khani, M. and Matsyura, A., Effect of altitude on essential oil composition and on glandular trichome density in three Nepeta species (N. sessilifolia, N. heliotropifolia and N. fissa). Mediterr. Bot., 2019, 40, 81–93.

  • Mahdavi, M., Jouri, M. H., Mahmoudi, J., Rezazadeh, F. and Mahzooni-Kachapi, S. S., Investigating the altitude effect on the quantity and quality of the essential oil in Tanacetum polycephalum Sch.-Bip. polycephalum in the Baladeh region of Nour, Iran. Chin. J. Nat. Med., 2013, 11, 553–559.

  • Jiao, Y., Sang, Y., Yang, L., Wang, Y. Q., Wu, Y. K., Du, R. W. and Yuan, L., Effects of fresh and composted Ageratina adenophora on physiology of three Solanaceae vegetables and yield and quality of pepper. Sci. Agric. Sin., 2016, 49, 874–884.

  • Balami, S., Thapa, L. B. and Jha, S. K., Effect of invasive Ageratina adenophora on species richness and composition of saprotrophic and pathogenic soil fungi. Biotropia, 2017, 24, 212–219.

  • Zhang, F., Guo, J., Chen, F., Liu, W. and Wan, F., Identification of volatile compounds released by leaves of the invasive plant Crofton weed (Ageratina adenophora, Compositae), and their inhibition of rice seedling growth. Weed Sci., 2012, 60, 205–211.

  • Hare, J. D., Ecological role of volatiles produced by plants in response to damage by herbivorous insects. Annu. Rev. Entomol., 2011, 56, 161–180.

  • Balami, S. and Thapa, L. B., Herbivory damage in native Alnus nepalensis and invasive Ageratina adenophora. Bot. Oriental., 2017, 11, 7–11.

  • Paré, P. W. and Tumlinson, J. H., Plant volatiles as a defense against insect herbivores. Plant Physiol., 1999, 121, 325–332.

  • Babushok, V. I., Linstrom, P. J. and Zenkevich, I. G., Retention indices for frequently reported compounds of plant essential oils. J. Phys. Chem. Ref. Data, 2011, 40, 043101.

  • Hedin, P. A., McKibben, G. H. and Dollar, D. A., Role of ground trash volatiles in the selection of hibernation sites by boll weevils. J. Agric. Food Chem., 2000, 48, 3673–3676.

  • Tellez, M. R., Canel, C., Rimando, A. M. and Duke, S. O., Differential accumulation of isoprenoids in glanded and glandless Artemisia annua L. Phytochemistry, 1999, 52, 1035–1040.

  • Furr, H. C., Zeng, S., Clifford, A. J. and Olson, J. A., Capillary gas chromatography of retinoids (vitamin A compounds) and apo-retinoids: determination of Kováts retention indices. J. Chromatogr., 1990, 527, 406–413.

  • Weyerstahl, P., Marschall, H., Thefeld, K. and Rustaiyan, A., Constituents of the essential oil of Tanacetum (syn. Chrysanthemum) fruticulosum Ledeb. from Iran. Flavour. Frag J., 1999, 14, 112–120.


Abstract Views: 226

PDF Views: 133




  • Chemical Constituents of Essential Oil from Leaves of an Invasive Weed Ageratina adenophora in Central Nepal

Abstract Views: 226  |  PDF Views: 133

Authors

Khadka Bahadur Pal
Department of Chemistry, Trichandra Campus, Tribhuvan University, Kathmandu, Nepal
Seeta Pathak
Central Department of Botany, Institute of Science and Technology, Tribhuvan University, Nepal
Tayer Mohamad Miya
Department of Food Technology and Quality Control, Government of Nepal, Kathmandu, Nepal
Tej Bahadur Darji
Department of Forest Resources, Ministry of Forest and Environment, Plant Research Center, Jumla, Nepal
Gunanand Pant
Department of Biology, Kailali Multiple Campus, Far Western University, Kailali, Nepal
Ramesh Raj Pant
Central Department of Environmental Science, Institute of Science and Technology, Tribhuvan University, Nepal
Lok Ranjan Bhatt
Biological Resource Unit, Nepal Academy of Science and Technology, Lalitpur, Nepal
Lal B. Thapa
Central Department of Botany, Institute of Science and Technology, Tribhuvan University, Nepal

Abstract


This study aims to identify volatile chemical components in the essential oil of an invasive plant, Ageratina adenophora, from Central Nepal. Leaf samples of A. adenophora were collected, and the chemical composition of essential oil was analysed by gas chromatography (GC) and GC–mass spectrometry (GC/MS). A total of 27 components in the oil were identified. The major compounds were cadinol, bisabolol and bornyl acetate. The amount of valencene, elemol and 2(1H)-naphthalenone, 4a,5,6,7,8,8a-hexahydro-6-[1-(hydroxymethyl)ethenyl]-4,8a-dimethyl-, [4ar(4a.alpha.,6.alpha.,8a.beta.]- varied with samples collected from different elevations. Five compounds, viz. cloven, β-gurjuene, β-cubebene, verbenone and cis-β-farnesene were rarely reported previously from A. adenophora. Being an invasive and medicinal plant, A. adenophora is found in varied ecological conditions. Further studies on the variation in amount and composition, phytotoxicity and benefits of such compounds are recommended.

Keywords


Ageratina adenophora, Chemical Constituents, Essential Oil, Gas Chromatography, Mass Spectrometry.

References





DOI: https://doi.org/10.18520/cs%2Fv124%2Fi6%2F757-761