Current Science

Open Access Open Access  Restricted Access Subscription Access

SSR Polymorphism and Genetics and Inheritance of Yield and Yield -Attributing Traits In Bitter Gourd (Momordica charantia L.)


Affiliations
1 ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India., India
2 ICAR-National Bureau of Plant Genetic Resources, New Delhi 110 012, India., India
 

The present study was conducted to ascertain the gene action and inheritance of yield and various yield-attri­bu­ting traits and to screen the simple sequence repeat (SSR) markers for parental polymorphism in bitter gourd (Momordica charantia L.). Two contrasting parents (S-2 and Pusa Purvi) were used for hybridization. Parental polymorphism was analysed using 100 SSR markers. DNA amplicon of size around 150–250 bp in both the parents was obtained for the 10 polymorphic SSR markers. These were used to ascertain the hybridity of F1 progeny, from which the subsequent generations were developed. Generation mean analysis, ABCD scaling test and joint scaling test were performed using the observations from six generations (parent-1, parent-2, F1, F2, back cross 1 and back cross 2). The additive effect of the genes was predominant for the quantitative traits like fruit length, diameter, weight, etc. Duplicate epistasis was observed for the majority of traits. The yield per plant was positively correlated with fruit length, diameter, weight and fruit number per plant. Chi-square test revealed the monogenic inheritance of qualitative traits (fruit tubercles and ridgeness, and shape at apex), in which ‘conspicuous tubercles’, ‘discontinuous ridgeness’ and ‘acute fruit shape at apex’ were dominant. These results reveal the complexity and polygenic nature of the yield. The findings obtained from the present study can be used for yield improvement in the future. For this, the polymorphic SSRs identified can also be used in genetic diversity studies, DNA fingerprinting, genetic mapping, genomics analysis, etc.

Keywords

Epistasis, Gene Interaction, Momordica Cha-Rantia L., Monogenic Inheritance, Polymorphism.
User
Notifications
Font Size

  • Behera, T. K., Behera, S., Bharathi, L. K., John, K. J., Simon, P. W. and Staub, J. E., Bitter gourd: botany, horticulture, breeding. Hortic. Rev., 2010, 37, 101–139.

  • Guo, D. L., Zhang, J. P., Xue, Y. M. and Hou, X. G., Isolation and characterization of 10 SSR markers of Momordica charantia (Cucurbitaceae). Am. J. Bot., 2012, 99(5), e182–e183.

  • Bharathi, L. K. et al., Production and preliminary characterization of inter-specific hybrids derived from Momordica species. Curr. Sci., 2012, 103, 178–186.

  • Cui, J., Cheng, J., Nong, D., Peng, J., Hu, Y., He, W. and Hu, K., Genome-wide analysis of simple sequence repeats in bitter gourd (Momordica charantia). Front. Plant Sci., 2017, 8, 1–10.

  • Doyle, J. J. and Doyle, J. L., A rapid DNA isolation procedure from small quantity of fresh leaf material. Phytochem. Bull., 1990, 119, 11–15.

  • Hayman, B. I., The separation of epistatic from additive and domi-nance variation in generation means. Heredity, 1958, 12, 371–390.

  • Hayman, B. I. and Mather, K., The description of genic interactions in continuous variation. Biometrics, 1955, 11(1), 69–82.

  • Kearsey, M. J. and Pooni, H. S., The Genetic Analysis of Quantita-tive Traits, Chapman and Hall, London, UK, 1996, p. 381.

  • Panse, V. G. and Sukhatme, P. V., Statistical Methods for Agricul-tural Workers, ICAR, New Delhi, 1985, p. 359.

  • Al-Mamuna, M. H., Harunur Rashid, M., Nazim Uddin, M., Rabiul Islam, M. and Asaduzzaman, M., Heterosis studies in bitter gourd. Int. J. Veg. Sci., 2016, 22(5), 442–450.

  • Rathod, V., Behera, T. K., Munshi, A. D., Jat, G. S. and Gaikwad, A. B., Genetic analysis for yield and its attributes in bitter gourd (Momordica charantia). Indian J. Agric. Sci., 2021, 91, 68–73.

  • Jinks, J. L. and Jones, R. M., Estimation of the components of het-erosis. Genetics, 1958, 43(2), 223–243.

  • Rani, K. R., Reddy, K. R. and Raju, C. S., Study of gene effects for yield and its component traits in bitter gourd (Momordica charantia L.) by generation mean analysis. Electron. J. Plant Breed., 2013, 4(3), 1237–1241.

  • Kumari, M., Behera, T. K., Munshi, A. D. and Talukadar, A., In-heritance of fruit traits and generation mean analysis for estimation of horticultural traits in bitter gourd. Indian J. Hortic., 2015, 72(1), 43–48.

  • Sirohi, P. S. and Choudhury, B., Inheritance of quantitative fruit characters in bitter gourd (Momordica charantia L.). Veg. Sci., 1980, 7(2), 102–107.

  • Kim, Z. H., Kim, Y. R. and Um, S. K., Inheritance of fruit weight in bitter-gourd (Momordica charantia L.). J. Korean Soc. Hortic. Sci., 1990, 31(3), 238–246.


Abstract Views: 222

PDF Views: 127




  • SSR Polymorphism and Genetics and Inheritance of Yield and Yield -Attributing Traits In Bitter Gourd (Momordica charantia L.)

Abstract Views: 222  |  PDF Views: 127

Authors

Ipsita Panigrahi
ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India., India
A. D. Munshi
ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India., India
S. S. Dey
ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India., India
A. B. Gaikwad
ICAR-National Bureau of Plant Genetic Resources, New Delhi 110 012, India., India
Manoranjan Senapati
ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India., India
Avinash Tomer
ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India., India

Abstract


The present study was conducted to ascertain the gene action and inheritance of yield and various yield-attri­bu­ting traits and to screen the simple sequence repeat (SSR) markers for parental polymorphism in bitter gourd (Momordica charantia L.). Two contrasting parents (S-2 and Pusa Purvi) were used for hybridization. Parental polymorphism was analysed using 100 SSR markers. DNA amplicon of size around 150–250 bp in both the parents was obtained for the 10 polymorphic SSR markers. These were used to ascertain the hybridity of F1 progeny, from which the subsequent generations were developed. Generation mean analysis, ABCD scaling test and joint scaling test were performed using the observations from six generations (parent-1, parent-2, F1, F2, back cross 1 and back cross 2). The additive effect of the genes was predominant for the quantitative traits like fruit length, diameter, weight, etc. Duplicate epistasis was observed for the majority of traits. The yield per plant was positively correlated with fruit length, diameter, weight and fruit number per plant. Chi-square test revealed the monogenic inheritance of qualitative traits (fruit tubercles and ridgeness, and shape at apex), in which ‘conspicuous tubercles’, ‘discontinuous ridgeness’ and ‘acute fruit shape at apex’ were dominant. These results reveal the complexity and polygenic nature of the yield. The findings obtained from the present study can be used for yield improvement in the future. For this, the polymorphic SSRs identified can also be used in genetic diversity studies, DNA fingerprinting, genetic mapping, genomics analysis, etc.

Keywords


Epistasis, Gene Interaction, Momordica Cha-Rantia L., Monogenic Inheritance, Polymorphism.

References





DOI: https://doi.org/10.18520/cs%2Fv124%2Fi9%2F1058-1064