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Phytoplasma on Sesame: Etiology, Insect Vectors, Molecular Characterization, Transmission and Integrated Management


Affiliations
1 ICAR-Indian Institute of Oilseeds Research, Rajendranagar, Hyderabad 500 030, India
 

Sesame phyllody disease is of serious concern in many sesame-growing areas. It significantly diminishes crop production, especially in warm environments causing up to 80% yield loss. We observed various symptoms of phyllody disease, viz. flowery phyllody, virescence, flower bud proliferation, ovivipary and cracking of seed capsules. Orosius albicinctus (Distant) was identified as the vector of sesame phyllody. Sesame phyllody phytoplasma 16SrI strain sequences of India, Egypt and Thailand were associated in one group; 16SrII strains were grouped separately, comprising sequences from India, Iran, Taiwan, Turkey and Oman. 16SrIX stains of Iran (MW27256, KF774193 and MW272565) and Turkey (KC139791) sequences were clearly distinguished from the phylogenetic tree. This result clearly shows the presence of different sesame phyllody phytoplasma strains and diversity in the Indian subcontinent. Sesame phytoplasma was effectively transmitted to the healthy plants from infected plants of sesame through O. albicinctus, grafting and dodder. The most sustainable and viable alternative for managing sesame phyllody can be an integrated strategy by combining cultural, host plant resistance, biological, physical and chemical methods. Sesame phyllody has become a potential threat to sesame cultivation. Hence, it is necessary to take steps to reduce its further spread. In this article, extensive details on distribution, taxonomy, symptomatology, etiology, transmission, molecular characterization, genetic diversity, host plant resistance and management methods on phytoplasma infecting sesame are provided.

Keywords

Host Plant Resistance, Insect Vectors, Molecular Characterization, Sesame Phyllody, Symptoms.
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  • FAO, Food and Agriculture Organization Statistical Database, 2020; http://faostat.fao.org/ (accessed on 28 October 2020).

  • Shyu, Y. S. and Hwang, L. S., Antioxidative activity of the crude extract of lignan glycosides from unroasted Burma black sesame meal. Food Res. Int., 2002, 35, 357–365.

  • Akhtar, K. P., Sarwar, G., Sarwar, N. and Elahi, M. T., Field evaluation of sesame germplasm against sesame phyllody disease. Pak. J. Bot., 2013, 45, 1085–1090.

  • Salehi, M., Esmailzadeh-Hosseini, S. A., Salehi, E. and Bertaccini, A., Genetic diversity and vector transmission of phytoplasmas associated with sesame phyllody in Iran. Folia Microbiol., 2017, 62, 99–109.

  • Vasudeva, R. S. and Sahambi, H. S., Phyllody in sesamum (Sesamum orientale L.). Indian Phytopathol., 1955, 8, 124–129.

  • Sertkaya, G., Martini, M., Musetti, R. and Osler, R., Detection and molecular characterization of phytoplasmas infecting sesame and solanaceous crops in Turkey. Bull. Insectol., 2007, 60, 141–142.

  • Rao, G. P., Nabi, S. U. and Madhupriya, Overview on a century progress in research on sesame phyllody disease. Phytopathogenic Mollicutes, 2015, 5, 74–83.

  • Kumar, P. and Mishra, U. S., Diseases of Sesamum indicum in Rohilkhand. Intensity and yield loss. Indian Phytopathol., 1992, 45, 121–122.

  • Storey, H. H., Report of the plant pathologist. In Fifth Annual Report of East African Agricultural Research Station for the year 1932–33, H.M. Stationery Office, United Kingdom, 1933.

  • Mazzani, B. and Malaguti, G., Filoidia en lochnera y sesamum. Agron. Trop. Maracay, 1952, 2, 59–63.

  • Desmits, M. and Laboucheix, J., Relationship between cotton phyllody and a similar disease of sesame. FAO Plant Prot. Bull., 1974, 22, 19–20.

  • Klein, M., Sesamum phyllody in Israel. Phytopathol. Z, 1977, 88, 166–171.

  • Kolte, S. J., Diseases of Annual Edible Oil Seed Crops, Vol. II, CRS Press, USA, 1985, p. 135.

  • Tamimi, K. M., Fattah, F. A. and Al-Hamdany, M. A., Shoot apex fasciation in Sesamum indicum associated with mycoplasma like organisms. Plant Pathol., 1989, 38, 300–304.

  • Nakashima, K., Chaleeprom, W., Wongkaew, P., Sirithorn, P. and Kato, S., Analysis of phyllody disease caused by phytoplasma in sesame and Richardia plants. J. Jpn. Int. Res. Center Agric. Sci., 1999, 7, 19–27.

  • Al Sakeiti, M. A., Al-Subhi, A. M., Saady, N. A. and Deadman, M. L., First report of witches’ broom disease of sesame (Sesamum indicum) in Oman. Plant Dis., 2005, 89, 530.

  • Akhtar, K. P., Dickinson, M., Sarwar, G., Jamil, F. F. and Haq, M. A., First report on the association of a 16SrII phytoplasma with sesame phyllody in Pakistan. Plant Pathol., 2008, 57, 771.

  • Win, N. K. K., Back, C. and Jung, H. Y., Phyllody phytoplasma infecting sesame in Myanmar belongs to group 16SrI and subgroup 16SrI-B. Trop. Plant Pathol., 2010, 35, 310–313.

  • Catal, M., Ikten, C., Yol, E., Ustun, R. and Uzun, B., First report of 16SrIX group phytoplasma associated with sesame phyllody in Turkey. Plant Dis., 2013, 97, 835.

  • Tseng, Y. W., Deng, W. L., Chang, C. J., Huang, J. W. and Jan, F. J., First report on the association of a 16SrII-A phytoplasma with sesame (Sesamum indicum) exhibiting abnormal stem curling and phyllody in Taiwan. Plant Dis., 2014, 98, 990.

  • Nabi, S., Madhupriya, Dubey, D. K., Rao, G. P., Baranwal, V. K. and Sharma, P., Characterization of phytoplasmas associated with sesame (Sesamum indicum) phyllody disease in North India utilizing multilocus genes and RFLP analysis. Indian Phytopathol., 2015, 68(1), 112–119.

  • Akhtar, K. P., Sarwar, G., Dickinson, M., Ahmad, M., Haq, M. A., Hameed, S. and Javeed, M., Sesame phyllody disease: symptomatology, etiology and transmission in Pakistan. Turk. J. Agri. For., 2009, 33, 477–486.

  • Salehi, M. and Izadpanah, K., Etiology and transmission of sesame phyllody in Iran. J. Phytopathol., 1992, 135, 37–47.

  • Pathak, D. M., Parakhia, A. M. and Akbari, L. F., Symptomatology and transmission of sesame phyllody disease caused by phytoplasma. J. Mycol. Plant Pathol., 2012, 42, 479–484.

  • Nakashima, K., Hayashi, T., Chaleeprom, W., Wongkaew, P. and Sirithorn, P., Detection of DNA of phytoplasmas associated with phyllody disease of sesame in Thailand. Ann. Phytopathol. Soc. Jpn., 1995, 61, 519–528.

  • Cengiz, I., Mursel, C., Engin, Y., Rustem, U., Seymus, F., Cengiz, T. and Bulent, U., Molecular identification, characterization and transmission of phytoplasmas associated with sesame phyllody in Turkey. Eur. J. Plant Pathol., 2014, 139, 217–229.

  • Nasu, S., Jensen, D. D. and Righardson, J., Electron microscopy of mycoplasma-like bodies associated with insect and plant hosts of peach Western-X disease. Virology, 1970, 41, 583–595.

  • Roland, J. C. and Roland, F., Atlas de Biologie vegetale, Vol. 2: Organisation des plantes a fleurs, Masson, Paris, 1983, 3rd edn, p. 118.

  • Nabi, S., Madhupriya, S., Dubey, D. K., Rao, G. P., Baranwal, V. K. and Sharma, P., Molecular characterization of ‘Candidatus Phytoplasma asteris’ subgroup I-B associated with sesame phyllody disease and identification of its natural vector and weed reservoir in India. Australas. Plant Pathol., 2015, 44, 289–297.

  • Cagirgan, M. I., Mbaye, N., Silme, R. S., Ouedraogo, N. and Topuz, H., The impact of climate variability on occurrence of sesame phyllody and symptomatology of the disease in a mediterranean environment. Turk. J. Field Crops, 2013, 18(1), 101–108.

  • Weintraub, P. G. and Beanland, L., Insect vectors of phytoplasmas. Annu. Rev. Entomol., 2006, 51, 91–111.

  • Esmailzadeh-Hosseini, S. A., Mirzaie, A., Jafari-Nodooshan, A. and Rahimian, H., The first report of transmission of a phytoplasma associated with sesame phyllody by Orosius albicinctus in Iran. Australas. Plant Dis. Notes, 2007, 2, 33–34.

  • Ranebennur, H. et al., Draft genome sequence of ‘Candidatus Phytoplasma australasia’, strain SS02 associated with sesame phyllody disease. 3 Biotech., 2022, 12(5), 1–5.

  • Pathak, D. M., Patel, D. R. and Patel, R. R., Control of sesame phyllody caused by PLO’S. Int. J. For. Crop Improv., 2013, 4(1), 47–48.

  • Ikten, C., Catal, M., Yol, E., Ustun, R., Furat, S., Toker, C. and Uzun, R., Molecular identification, characterization and transmission of phytoplasmas associated with sesame phyllody in Turkey. Eur. J. Plant Pathol., 2014, 139, 217–229.

  • Gogoi, S. H., Kalita, M. K. and Nath, P. D., Biological characterization of sesamum phyllody disease in Assam, India. Int. J. Curr. Microbiol. Appl. Sci., 2017, 6(11), 1862–1875.

  • Hogenhout, S. A., Oshima, K., Ammar, E., Kakizawa, S., Kingdom, H. N. and Namba, S., Phytoplasmas: bacteria that manipulate plants and insects. Mol. Plant Pathol., 2008, 9(4), 403–423.

  • Christensen, N. M., Nicolaisen, M., Hansen, M. and Schultz, A., Distribution of phytoplasmas in infected plants as revealed by real time PCR and bioimaging. Mol. Plant. Microbe Interact., 2004, 17, 1175–1184.

  • Kumari, S., Nagendran, K., Rai, A. B., Singh, B., Rao, G. P. and Bertaccini, A., Global status of phytoplasma diseases in vegetable crops. Front. Microbiol., 2019, 10, 1349; doi:10.3389/fmicb.2019.01349.

  • Rhind, D., A note on photoperiodism in Sesamum. Indian J. Agric. Sci., 1935, 5, 729–736.

  • Joshi, A. B., Sesamum. Indian Central Oilseeds Committee, Hyderabad, 1961.

  • Esmailzadeh-Hosseini, S. A. et al., Status of sesame phyllody and its control methods in Yazd, Iran. Phytopathogenic Mollicutes, 2015, 5(Suppl. 1), S119–S120.

  • Bisht, I. S., Mahajan, R. K., Loknathan, T. R. and Agarwal, R. C., Diversity in Indian sesame collection and stratification of germplasm accessions in different diversity groups. Genet. Resour. Crop Evol., 1998, 45, 325–335; doi:10.1023/A:1008652420477.

  • Mehetre, S. S., Chatge, R. D. and Lad, S. K., Wild Sesamum mulayanums: a source of multiple disease resistance. Ann. Agric. Res., 1993, 15, 243–244.

  • Saravanan, S. and Nandarajan, N., Pathogenicity of mycoplasma like organism of sesame (Sesamum indicum L.) and its wild relatives. Agric. Sci. Digest, 2005, 25, 77–78.

  • AICRP Sesame and Niger, Annual Report, 2018–19. Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur, 2019.

  • Gupta, K. N., Ranganatha, A. R. G., Pandey, A. K., Sharma, S. and Panse, R., Field efficacy of bio-pesticides and insecticides for management of phyllody disease in sesame. In Third International Conference on Agriculture and Horticulture, Hyderabad, India, 2014, p. 235.

  • Markad, H. N., Magar, S. J., Navale, M. D. and Dhawan, S. S., Efficacy of insecticides, oils and antibiotics against management of sesamum phyllody. Int. J. Chem. Stud., 2018, 6(5), 269–271.

  • Kumar, R., Ali, S. and Kumar, S., Impact of botanical pesticides for the management of Antigastra catalaunalis Dup. in Sesamum indicum L. Mol. Entomol., 2012, 3, 1–3.

  • Naik, M. K., Savitha, M. K., Amaresh, A. S. and Gurikar, C., Integrated management of phyllody of sesame. In 35th National Conference and Symposium of ISMPPAt, Akola, 2014.

  • Gupta, K. N., Ranganatha, A. R. G., Pandey, A. K., Sharma, S. and Panse, R., Field efficacy of bio-pesticides and insecticides for management of phyllody disease in sesame. Agrotechnology, 2015, 2, 4; http://dx.doi.org/10.4172/2168-9881.S1.012.

  • Srinivasulu, B. and Narayanaswamy, P., Four-tier technique for screening sesamum entries against phyllody disease. Oil Crops Newsl., 1992, 9, 38–40.

  • Singh, P. K., Akram, M., Vajpeyi, M., Srivastava, R. L., Kumar, K. and Naresh, R., Screening and development of resistant sesame varieties against phytoplasma. Bull. Insectol., 2007, 60(2), 303–304.

  • ICAR-IIOR, Annual Report 2018–19, ICAR-Indian Institute of Oilseeds Research, Hyderabad, 2019.

  • Rajput, S. D. and Raghuvanshi, K. S., New technique of field resistance screening of sesame cultivars against phyllody disease transmitted by leaf hopper. Int. J. Curr. Sci., 2017, 20, 47–53.

  • Taye, W., Alemu, Z., Getahun, S., Abate, S., Seid, N. and Hemba, M., Evaluation of sesame varieties for phyllody disease resistance. Acad. Res. J. Agric. Sci. Res., 2019, 7(3), 110–118.

  • Selvanarayanan, V. and Selvamuthukumaran, T., Field resistance of sesame cultivars against phyllody disease transmitted by Orosius albicinctus Distant. Sesame Safflower Newsl., 2000, 15, 71–74.


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  • Phytoplasma on Sesame: Etiology, Insect Vectors, Molecular Characterization, Transmission and Integrated Management

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Authors

T. Boopathi
ICAR-Indian Institute of Oilseeds Research, Rajendranagar, Hyderabad 500 030, India
M. Sujatha
ICAR-Indian Institute of Oilseeds Research, Rajendranagar, Hyderabad 500 030, India
M. Santha Lakshmi Prasad
ICAR-Indian Institute of Oilseeds Research, Rajendranagar, Hyderabad 500 030, India
P. Duraimurugan
ICAR-Indian Institute of Oilseeds Research, Rajendranagar, Hyderabad 500 030, India
K. Sakthivel
ICAR-Indian Institute of Oilseeds Research, Rajendranagar, Hyderabad 500 030, India
K. T. Ramya
ICAR-Indian Institute of Oilseeds Research, Rajendranagar, Hyderabad 500 030, India
A. L. Rathnakumar
ICAR-Indian Institute of Oilseeds Research, Rajendranagar, Hyderabad 500 030, India

Abstract


Sesame phyllody disease is of serious concern in many sesame-growing areas. It significantly diminishes crop production, especially in warm environments causing up to 80% yield loss. We observed various symptoms of phyllody disease, viz. flowery phyllody, virescence, flower bud proliferation, ovivipary and cracking of seed capsules. Orosius albicinctus (Distant) was identified as the vector of sesame phyllody. Sesame phyllody phytoplasma 16SrI strain sequences of India, Egypt and Thailand were associated in one group; 16SrII strains were grouped separately, comprising sequences from India, Iran, Taiwan, Turkey and Oman. 16SrIX stains of Iran (MW27256, KF774193 and MW272565) and Turkey (KC139791) sequences were clearly distinguished from the phylogenetic tree. This result clearly shows the presence of different sesame phyllody phytoplasma strains and diversity in the Indian subcontinent. Sesame phytoplasma was effectively transmitted to the healthy plants from infected plants of sesame through O. albicinctus, grafting and dodder. The most sustainable and viable alternative for managing sesame phyllody can be an integrated strategy by combining cultural, host plant resistance, biological, physical and chemical methods. Sesame phyllody has become a potential threat to sesame cultivation. Hence, it is necessary to take steps to reduce its further spread. In this article, extensive details on distribution, taxonomy, symptomatology, etiology, transmission, molecular characterization, genetic diversity, host plant resistance and management methods on phytoplasma infecting sesame are provided.

Keywords


Host Plant Resistance, Insect Vectors, Molecular Characterization, Sesame Phyllody, Symptoms.

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DOI: https://doi.org/10.18520/cs%2Fv125%2Fi4%2F383-391