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Fine mapping of consistent quantitative trait loci for yield under drought stress using rice (Oryza sativa) recombinant inbred lines adapted to rainfed environment


Affiliations
1 Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore 641 003, India
 

Drought stress is a serious constraint, especially in rainfed rice production, and breeding for drought tolerance by selection based on yield under stress, though effective, is slow Mapping quantitative trait loci (QTLs) for yield and its components under drought stress predominant in rainfed target populations of environment (TPE) will help overcome this limitation. In the present study, a subset of 143 F8 and F9 recombinant inbred (RI) lines derived from IR62266-42-6-2 (IR62266), a high-yielding indica ecotype and Norungan, a landrace from TPE, was used to map QTLs for yield and its components under drought predominant in TPE. A large effect yield QTL observed under drought stress in TPE was consistent across two years with a phenotypic variation of 31.3% and 37.9% and additive effect of 629.2 and 424.9 kg/ha Further, this region was fine-mapped to 94.0 kb with positive effect on grain yield under stress.

Keywords

Comparative genomics, drought stress, fine mapping, quantitative trait locus, rice.
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  • Fine mapping of consistent quantitative trait loci for yield under drought stress using rice (Oryza sativa) recombinant inbred lines adapted to rainfed environment

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Authors

C. Muthukumar
Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore 641 003, India
V. Deshmukh Vivek
Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore 641 003, India
R. Poornima
Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore 641 003, India
S. Kavitha
Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore 641 003, India
V. Gayathri
Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore 641 003, India
R. Chandra Babu
Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore 641 003, India

Abstract


Drought stress is a serious constraint, especially in rainfed rice production, and breeding for drought tolerance by selection based on yield under stress, though effective, is slow Mapping quantitative trait loci (QTLs) for yield and its components under drought stress predominant in rainfed target populations of environment (TPE) will help overcome this limitation. In the present study, a subset of 143 F8 and F9 recombinant inbred (RI) lines derived from IR62266-42-6-2 (IR62266), a high-yielding indica ecotype and Norungan, a landrace from TPE, was used to map QTLs for yield and its components under drought predominant in TPE. A large effect yield QTL observed under drought stress in TPE was consistent across two years with a phenotypic variation of 31.3% and 37.9% and additive effect of 629.2 and 424.9 kg/ha Further, this region was fine-mapped to 94.0 kb with positive effect on grain yield under stress.

Keywords


Comparative genomics, drought stress, fine mapping, quantitative trait locus, rice.

References





DOI: https://doi.org/10.18520/cs%2Fv109%2Fi5%2F910-917