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Genetic Diversity Assessment of Valeriana jatamansi Jones Using Microsatellites Markers


Affiliations
1 G.B. Pant Institute of Himalayan Environment and Development Kosi-Katarmal, Almora 263 643, India
2 Bhabha Atomic Research Center, Mumbai 400 005, India
 

Valeriana jatamansi (family Valerianaceae) is a highvalue medicinal plant used in traditional and modern medicine. In the present study, 25 populations (151 genotypes) of V. jatamansi were collected from Uttarakhand, India and investigated using nuclear and chloroplast SSR markers. Six nuclear and seven chloroplast polymorphic SSR primer pairs were used to evaluate genetic variability and population relatedness. These primer pairs have generated 55 fragments (27 nuclear, 33 chloroplast). The number of alleles per locus ranged from 3 to 7. Expected heterozygosity of the 25 V. jatamansi population was 0.108-0.222 with a mean of 0.165 for nuclear SSR markers and 0.147- 0.265 with a mean of 0.215 for chloroplast SSR markers. Based on AMOVA analysis, 6.0% (P = <0.001) of total genetic variation was found. Nuclear SSR markers exhibited highest genetic diversity in samples collected from 1501 to 1800 m amsl altitudinal range and from pine forest habitat. In case of cpSSR, samples collected from 2101 to 2400 m amsl and grassland habitat exhibited highest diversity. These markers could be helpful in the identification and prioritization of genetically diverse populations/individuals for conservation and utilizing them in genetic improvement of V. jatamansi.

Keywords

Conservation, Gene Flow, Genetic Diversity, Microsatellites, Valeriana Jatamansi.
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  • Genetic Diversity Assessment of Valeriana jatamansi Jones Using Microsatellites Markers

Abstract Views: 446  |  PDF Views: 197

Authors

Arun Kumar Jugran
G.B. Pant Institute of Himalayan Environment and Development Kosi-Katarmal, Almora 263 643, India
Indra D. Bhatt
G.B. Pant Institute of Himalayan Environment and Development Kosi-Katarmal, Almora 263 643, India
Suvendu Mondal
Bhabha Atomic Research Center, Mumbai 400 005, India
Ranbeer S. Rawal
G.B. Pant Institute of Himalayan Environment and Development Kosi-Katarmal, Almora 263 643, India
Shyamal K. Nandi
G.B. Pant Institute of Himalayan Environment and Development Kosi-Katarmal, Almora 263 643, India

Abstract


Valeriana jatamansi (family Valerianaceae) is a highvalue medicinal plant used in traditional and modern medicine. In the present study, 25 populations (151 genotypes) of V. jatamansi were collected from Uttarakhand, India and investigated using nuclear and chloroplast SSR markers. Six nuclear and seven chloroplast polymorphic SSR primer pairs were used to evaluate genetic variability and population relatedness. These primer pairs have generated 55 fragments (27 nuclear, 33 chloroplast). The number of alleles per locus ranged from 3 to 7. Expected heterozygosity of the 25 V. jatamansi population was 0.108-0.222 with a mean of 0.165 for nuclear SSR markers and 0.147- 0.265 with a mean of 0.215 for chloroplast SSR markers. Based on AMOVA analysis, 6.0% (P = <0.001) of total genetic variation was found. Nuclear SSR markers exhibited highest genetic diversity in samples collected from 1501 to 1800 m amsl altitudinal range and from pine forest habitat. In case of cpSSR, samples collected from 2101 to 2400 m amsl and grassland habitat exhibited highest diversity. These markers could be helpful in the identification and prioritization of genetically diverse populations/individuals for conservation and utilizing them in genetic improvement of V. jatamansi.

Keywords


Conservation, Gene Flow, Genetic Diversity, Microsatellites, Valeriana Jatamansi.



DOI: https://doi.org/10.18520/cs%2Fv109%2Fi7%2F1273-1282