Open Access
Subscription Access
Evaluation of Peptide-Based Approach for Estimation of Nse and S-100ββ towards the Development of a Cost-Effective Test for Prognosis of AIS Patients
Here we compare the in-house ELISA technique for estimation of neuron-specific enolase (NSE) and S-100ββ (glial-specific protein) peptides with a commercial kit in serum samples of acute ischaemic stroke (AIS) patients. Nineteen improved and four expired AIS patients were included for the study. NSE concentrations were significantly higher (P < 0.05) in expired as compared with improved AIS patients by both inhouse ELISA and commercial kit method. Estimation of S-100ββ by only in-house ELISA showed significantly high (P < 0.05) levels in expired AIS patients. Peptide-based estimation of NSE and S-100ββ may be used for prognosis of AIS patients.
Keywords
Acute Ischaemic Stroke, Glial-Specific Protein, Neurological Disease, Neuron-specific Enolase, Prognosis.
User
Font Size
Information
- Johnsingh, A. J. T., Bear conservation in India. J. Bombay Nat. Hist. Soc., 2003, 100, 190–201.
- Yoganand, K., Rice, C. G., Johnsingh, A. J. T. and Seidensticker, J., Is the sloth bear in India secure? A preliminary report on distribution, threats and conservation requirements. J. Bombay Nat. Hist. Soc., 2006, 103, 57–66.
- Chauhan, N. S., The status of sloth bears in India. In Understanding Asian Bears to Secure their Future, Japan Bear Network, Ibaraki, Japan, 2006, pp. 26–34.
- Chauhan, N. P. S., Bargali, H. S. and Akhtar, N., Activity patterns of sloth bear in fragmented and disturbed areas of Bilaspur Forest Division, Chattisgarh, India. In Proceedings of the 15th International Conference on Bear Research and Management, San Diego, California, USA, 2004.
- Garshelis, D. L., Ratnayeke, S. and Chauhan, N. P. S., Melursus ursinus. The IUCN Red List of Threatened Species v. 2014.3, 2008; http://www.iucnredlist.org (accessed on 25 November 2014).
- Rajpurohit, K. S. and Krausman, P. R., Human–sloth-bear conflicts in Madhya Pradesh, India. Wildl. Soc. Bull., 2000, 28, 393– 399.
- Yoganand, K., Rice, C. G. and Johnsingh, A. J. T., Sloth bear Melursus ursinus. In Mammals of South Asia (eds Johnsingh, A. J. T. and Manjrekar, N.), University Press (India), Private Limited, Hyderabad, India, 2013, pp. 438–456.
- Bawa, K. S., Das, A., Krishnaswamy, J. and Karanth, K. U., Kumar, N. S. and Rao, M., CEPF Western Ghats and Sri Lanka Ecosystem Profile, 2007.
- Bhupathy, S., Srinivas, G., Sathishkumar, N., Murugesan, M., Babu, S., Suganthasakthivel, R. and Sivakumar, P., Diversity and conservation of selected biota of the Meghamalai landscape, Western Ghats, India. Curr. Sci., 2012, 102, 590–595.
- Srinivas, G. and Bhupathy, S., Anurans of the Meghamalai Landscape, Western Ghats, India. J. Threat. Taxa, 2013, 5, 4973–4978.
- Bhupathy, S. and Sathishkumar, N., Status of reptiles in Meghamalai and its environs, Western Ghats, Tamil Nadu, India. J. Threat. Taxa, 2013, 5, 4953–4961.
- Babu, S. and Bhupathy, S., Birds of Meghamalai Landscape, southern Western Ghats, India. J. Threat. Taxa, 2013, 5, 4962– 4972.
- Hines, J. E., Nichols, J. D., Royle, J. A., MacKenzie, D. I., Gopalaswamy, A. M., Samba Kumar, N. and Karanth, K. U., Tigers on trails: occupancy modeling for cluster sampling. Ecol. Appl., 2010, 20, 1456–1466.
- Dombois, M. D. and Ellenberg, H., Aims and Methods of Vegetation Ecology, John Wiley, New York, 1974.
- MacKenzie, D. I., Nichols, J. D., Royle, J. A., Pollock, K. H., Bailey, L. L. and Hines, J. E., Occupancy Estimation and Modelling: Inferring Patterns and Dynamics of Species Occurrence, Elsevier, London, 2006.
- Burnham, K. P. and Anderson, D. R., Model Selection and Multimodel Inference: A Practical Information-Theoretic Approach, Second Edition, Springer-Verlag, New York, 2002, 2nd edn.
- Joshi, A. R., Garshelis, D. L. and Smith, J. L., Seasonal and habitatrelated diets of sloth bears in Nepal. J. Mammol., 1997, 78, 584–597.
- Sreekumar, P. G. and Balakrishnan, M., Seed dispersal by the sloth bear (Melursus ursinus) in South India. Biotropica, 2002, 34, 474–477.
- Akhtar, N., Bargali, H. S. and Chauhan, N. P. S., Sloth bear habitat use in disturbed and unprotected areas of Madhya Pradesh, India. Ursus, 2004, 15, 203–211.
- Ramesh, T., Kalle, R., Sankar, K. and Qureshi, Q., Factors affecting habitat patch use by sloth bears in Mudumalai Tiger Reserve, Western Ghats, India. Ursus, 2012, 23, 78–85.
- Das, S., Dutta, S., Sen, S., Jijumon, A., Babu, S., Kumara, H. N. and Singh, M., Identifying regions for conservation of sloth bears through occupancy modelling in north-eastern Karnataka, India. Ursus, 2012, 25, 111–120.
- Krishna, Y. C., Krishnaswamy, J. and Kumar, K. S., Habitat factors affecting site occupancy and relative abundance of four‐horned antelope. J. Zool., 2008, 276, 63–70.
- Baskaran, N., An ecological investigation on the dietary composition and habitat utilisation of sloth bear (Melursus ursinus) at Mudumalai Wildlife Sanctuary, Tamil Nadu (South India), thesis, Bharathidasan University, India, 1990.
- Garshelis, D. L., Joshi, A. R., Smith, J. L. D. and Rice, C. G., Sloth bear conservation action plan (Melursus ursinus). In Bears. Status Survey and Conservation Action Plan (eds Servheen, C., Herrero, S. and Peyton, B.), IUCN/SSC Bear and Polar Bear Specialist Groups, IUCN – The World Conservation Union, Gland, Switzerland, 1999, pp. 225–240.
- Joshi, A. R., Garshelis, D. L. and Smith, J. L., Home ranges of sloth bears in Nepal: implications for conservation. J. Wildl. Manage., 1995, 59, 204–214.
- Balakrishnan, M. and Easa, P. S., Habitat preferences of the larger mammals in the Parambikulam Wildlife Sanctuary, Kerala, India. Biol. Conserv., 1986, 37, 191–200.
- Prasad, A. E., Tree community change in a tropical dry forest: the role of road and exotic plant invasion. Environ. Conserv., 2009, 36, 201–207.
- Kumara, H. N., Sasi, R., Suganthasakthivel, R. and Srinivas, G., Distribution, abundance and conservation of primates in the Highwavy Mountains of Western Ghats, Tamil Nadu, India and conservation prospects for lion-tailed macaques. Curr. Sci., 2011, 100, 1063–1067.
Abstract Views: 363
PDF Views: 157