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Draft Genome Sequence of Cercospora canescens: A Leaf Spot Causing Pathogen


Affiliations
1 Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi 221 005, India
2 Centre for Bioinformatics, School of Biotechnology, Banaras Hindu University, Varanasi 221 005, India
3 ICAR-Central Plantation Crops Research Institute, Kudlu, P.O. Kasargod 671 124, India
 

Cercospora canescens (Ellis and Martin) is a hemibiotrophic pathogen causing leaf spot disease on mungbean (Vigna radiata L). Genome sequence (∼33.97 Mb) assembled in 8239 contigs with 10627 protein coding genes. A total of 2842 proteins were identified as homologous of 223 predicted and 7562 putative uncharacterized involved in biological processes, molecular functions. The identified proteins are mainly involved in infection process used to compromise nutrients or destroy host tissues gycosidases, transposases, cytochrome P450s, genes codes to signal transduction, cell wall breakdown, transporters, host stomata perception, adhesion, polyketide synthase and cercosporin. A total of 528 simple sequence repeats were also identified from the genome sequence assembly of C. canescens. This study provides insights into pathogenic mechanism and a better understanding of virulence differentiation of C. canescens. It will also help in identification of similarity and differences in regions among the genomes of different species of Cercospora.

Keywords

Cercospora canescens, Functional Annotation, Gene Prediction, Sequencing and Assembly, Vigna radiata.
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  • Draft Genome Sequence of Cercospora canescens: A Leaf Spot Causing Pathogen

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Authors

Ramesh Chand
Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi 221 005, India
Chhattar Pal
Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi 221 005, India
Vineeta Singh
Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi 221 005, India
Manoj Kumar
Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi 221 005, India
Vinay Kumar Singh
Centre for Bioinformatics, School of Biotechnology, Banaras Hindu University, Varanasi 221 005, India
Chowdappa Pallem
ICAR-Central Plantation Crops Research Institute, Kudlu, P.O. Kasargod 671 124, India

Abstract


Cercospora canescens (Ellis and Martin) is a hemibiotrophic pathogen causing leaf spot disease on mungbean (Vigna radiata L). Genome sequence (∼33.97 Mb) assembled in 8239 contigs with 10627 protein coding genes. A total of 2842 proteins were identified as homologous of 223 predicted and 7562 putative uncharacterized involved in biological processes, molecular functions. The identified proteins are mainly involved in infection process used to compromise nutrients or destroy host tissues gycosidases, transposases, cytochrome P450s, genes codes to signal transduction, cell wall breakdown, transporters, host stomata perception, adhesion, polyketide synthase and cercosporin. A total of 528 simple sequence repeats were also identified from the genome sequence assembly of C. canescens. This study provides insights into pathogenic mechanism and a better understanding of virulence differentiation of C. canescens. It will also help in identification of similarity and differences in regions among the genomes of different species of Cercospora.

Keywords


Cercospora canescens, Functional Annotation, Gene Prediction, Sequencing and Assembly, Vigna radiata.

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DOI: https://doi.org/10.18520/cs%2Fv109%2Fi11%2F2103-2110