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Monitoring of Dengue and Chikungunya Viruses in Field-Caught Aedes aegypti (Diptera: Culicidae) in Surat City, India


Affiliations
1 Medical Entomology and Zoology Department, Microbial Containment Complex, National Institute of Virology, 130/1, Sus Road, Pashan, Pune 411 021, India
2 Vector Borne Diseases Control Department, Surat Municipal Corporation, Surat 396 003, India
3 Dengue Group, National Institute of Virology, 20/A, Ambedkar Road, Pune 411 001, India
 

A virological surveillance was carried out in various places of Surat Metropolitan Region (SMR) to monitor the chikungunya and dengue activity in Aedes aegypti populations. A total of 556 pools constituting 4918 Ae. aegypti mosquitoes were collected and processed in the laboratory for the detection of chikungunya virus (CHIKV) and dengue virus (DENV) using Immuno Fluorescence Assay (IFA) and reverse transcription polymerase chain reaction (RT-PCR). Thirteen pools were found positive for DENV and one pool was found positive for CHIKV. Molecular phylogeny results revealed that DENV-3 GIII, DENV-4 GI and CHIKV ECSA are circulating in Surat. Based on GIS information and climate conditions, the risk map for Ae. aegypti and DENV infections was predicted. Risk maps suggest that the central and western parts of Surat are at high risk of Ae. aegypti spread and associated DENV infection.

Keywords

Arboviruses, GIS Mapping, Phylogenetic Analysis, Risk Map, Surveillance.
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  • Monitoring of Dengue and Chikungunya Viruses in Field-Caught Aedes aegypti (Diptera: Culicidae) in Surat City, India

Abstract Views: 435  |  PDF Views: 120

Authors

Mandar S. Paingankar
Medical Entomology and Zoology Department, Microbial Containment Complex, National Institute of Virology, 130/1, Sus Road, Pashan, Pune 411 021, India
Mangesh D. Gokhale
Medical Entomology and Zoology Department, Microbial Containment Complex, National Institute of Virology, 130/1, Sus Road, Pashan, Pune 411 021, India
Keshav G. Vaishnav
Vector Borne Diseases Control Department, Surat Municipal Corporation, Surat 396 003, India
Paresh S. Shah
Dengue Group, National Institute of Virology, 20/A, Ambedkar Road, Pune 411 001, India

Abstract


A virological surveillance was carried out in various places of Surat Metropolitan Region (SMR) to monitor the chikungunya and dengue activity in Aedes aegypti populations. A total of 556 pools constituting 4918 Ae. aegypti mosquitoes were collected and processed in the laboratory for the detection of chikungunya virus (CHIKV) and dengue virus (DENV) using Immuno Fluorescence Assay (IFA) and reverse transcription polymerase chain reaction (RT-PCR). Thirteen pools were found positive for DENV and one pool was found positive for CHIKV. Molecular phylogeny results revealed that DENV-3 GIII, DENV-4 GI and CHIKV ECSA are circulating in Surat. Based on GIS information and climate conditions, the risk map for Ae. aegypti and DENV infections was predicted. Risk maps suggest that the central and western parts of Surat are at high risk of Ae. aegypti spread and associated DENV infection.

Keywords


Arboviruses, GIS Mapping, Phylogenetic Analysis, Risk Map, Surveillance.



DOI: https://doi.org/10.18520/cs%2Fv106%2Fi11%2F1559-1567