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Efficacy of Two Dominant Marker Systems, ISSR and TE-AFLP for Assessment of Genetic Diversity in Biodiesel Species Pongamia Pinnata


Affiliations
1 The Energy and Resources Institute, IHC Complex, Lodhi Road, New Delhi 110 003, India
2 Rungta College of Science and Technology, Durg 491 001, India
 

The extent of genetic diversity was assessed in 12 Pongamia accessions from different regions of Delhi and surrounding areas using two dominant markers, namely ISSR and three endonuclease AFLP (TEAFLP). Five ISSR primers and two TE-AFLP primer combinations generated a total of 12 and 48 polymorphic bands respectively. The Jaccard's dissimilarity coefficient ranged from 0 to 0.90 for ISSR and from 0 to 0.67 for TE-AFLP markers. The polymorphic information content of both markers was equal. However, TE-AFLP had much higher values of marker index and resolving power compared to those obtained for ISSR markers. This study demonstrates the usefulness of dominant markers like ISSR and TE-AFLP for assessment of genetic diversity in Pongamia for which microsatellites markers are still not available. However, high multiplex ratio, easy scorability and other high band attributes of TE-AFLP markers make them more suitable compared to ISSR for genetic diversity analysis.

Keywords

Dominant Markers, Genetic Diversity, Pongamia Accessions.
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  • Efficacy of Two Dominant Marker Systems, ISSR and TE-AFLP for Assessment of Genetic Diversity in Biodiesel Species Pongamia Pinnata

Abstract Views: 388  |  PDF Views: 134

Authors

Shyam Sundar Sharma
The Energy and Resources Institute, IHC Complex, Lodhi Road, New Delhi 110 003, India
Keshaw Aadil
Rungta College of Science and Technology, Durg 491 001, India
Madan Singh Negi
The Energy and Resources Institute, IHC Complex, Lodhi Road, New Delhi 110 003, India
Shashi Bhushan Tripathi
The Energy and Resources Institute, IHC Complex, Lodhi Road, New Delhi 110 003, India

Abstract


The extent of genetic diversity was assessed in 12 Pongamia accessions from different regions of Delhi and surrounding areas using two dominant markers, namely ISSR and three endonuclease AFLP (TEAFLP). Five ISSR primers and two TE-AFLP primer combinations generated a total of 12 and 48 polymorphic bands respectively. The Jaccard's dissimilarity coefficient ranged from 0 to 0.90 for ISSR and from 0 to 0.67 for TE-AFLP markers. The polymorphic information content of both markers was equal. However, TE-AFLP had much higher values of marker index and resolving power compared to those obtained for ISSR markers. This study demonstrates the usefulness of dominant markers like ISSR and TE-AFLP for assessment of genetic diversity in Pongamia for which microsatellites markers are still not available. However, high multiplex ratio, easy scorability and other high band attributes of TE-AFLP markers make them more suitable compared to ISSR for genetic diversity analysis.

Keywords


Dominant Markers, Genetic Diversity, Pongamia Accessions.



DOI: https://doi.org/10.18520/cs%2Fv106%2Fi11%2F1576-1580