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Distribution and Molecular Characterization of Wolbachia Endosymbionts in Odonata (Insecta) from Central India by Multigene Approach


Affiliations
1 National Centre for Cell Science, University of Pune Campus, Ganeshkhind, Pune 411 007, India
2 Microbial Culture Collection, National Centre for Cell Science, University of Pune Campus, Ganeshkhind, Pune 411 007, India
3 Department of Zoology, Hislop College, Nagpur 440 001, India
4 Department of Microbiology, Moolji Jaitha College, Jalgaon 425 002, India
 

Wolbachia are maternally inherited bacterial endo-symbionts of arthropods distributed among a wide range of hosts. It is now well known that they induce reproductive manipulations in their arthropod hosts by various phenotypic effects. The objective of the present study was to investigate Wolbachia infection among the insect order Odonata comprising 16 species from 5 families. Fifteen odonate species representing five families were found to harbour Wolbachia with the overall infection rate of 70%, out of which four-teen species are reported for the first time. According to multilocus sequence typing (MLST) data and phylo-genetic analysis, all odonate Wolbachia species belong to supergroup F, except Trithemis pallindinervis, which belongs to supergroup B. MLST data reveal 20 new, highly similar STs (99.32  0.34). We found a high rate of Wolbachia infection in Odonata of India, which indicates importance of this association. The charac-terization of these Wolbachia strains promises to lead to a deeper insight into this interaction, which is essential for further studies based on their phenotypic effects. The study suggests that all the characterized Wolbachia STs are totally new and arise as a result of point mutation.

Keywords

Multilocus Sequence Typing, Phenotypic Effects, Point Mutations.
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  • Distribution and Molecular Characterization of Wolbachia Endosymbionts in Odonata (Insecta) from Central India by Multigene Approach

Abstract Views: 356  |  PDF Views: 148

Authors

Rahul C. Salunkhe
National Centre for Cell Science, University of Pune Campus, Ganeshkhind, Pune 411 007, India
Dhiraj P. Dhotre
Microbial Culture Collection, National Centre for Cell Science, University of Pune Campus, Ganeshkhind, Pune 411 007, India
Bipinchandra K. Salunke
National Centre for Cell Science, University of Pune Campus, Ganeshkhind, Pune 411 007, India
Vikas S. Patil
Microbial Culture Collection, National Centre for Cell Science, University of Pune Campus, Ganeshkhind, Pune 411 007, India
Vaibhav Mahale
National Centre for Cell Science, University of Pune Campus, Ganeshkhind, Pune 411 007, India
Raymond J. Andrew
Department of Zoology, Hislop College, Nagpur 440 001, India
Milind S. Patole
National Centre for Cell Science, University of Pune Campus, Ganeshkhind, Pune 411 007, India
Ketan P. Narkhede
Department of Microbiology, Moolji Jaitha College, Jalgaon 425 002, India
Yogesh S. Shouche
Microbial Culture Collection, National Centre for Cell Science, University of Pune Campus, Ganeshkhind, Pune 411 007, India

Abstract


Wolbachia are maternally inherited bacterial endo-symbionts of arthropods distributed among a wide range of hosts. It is now well known that they induce reproductive manipulations in their arthropod hosts by various phenotypic effects. The objective of the present study was to investigate Wolbachia infection among the insect order Odonata comprising 16 species from 5 families. Fifteen odonate species representing five families were found to harbour Wolbachia with the overall infection rate of 70%, out of which four-teen species are reported for the first time. According to multilocus sequence typing (MLST) data and phylo-genetic analysis, all odonate Wolbachia species belong to supergroup F, except Trithemis pallindinervis, which belongs to supergroup B. MLST data reveal 20 new, highly similar STs (99.32  0.34). We found a high rate of Wolbachia infection in Odonata of India, which indicates importance of this association. The charac-terization of these Wolbachia strains promises to lead to a deeper insight into this interaction, which is essential for further studies based on their phenotypic effects. The study suggests that all the characterized Wolbachia STs are totally new and arise as a result of point mutation.

Keywords


Multilocus Sequence Typing, Phenotypic Effects, Point Mutations.



DOI: https://doi.org/10.18520/cs%2Fv108%2Fi5%2F971-978