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Compensatory Effects of Medicinal Plants of Pakistan upon Prolongation of Coagulation Assays Induced by Naja naja karachiensis Bite


Affiliations
1 Department of Pharmacy, COMSATS Institute of Information Technology, Abbottabad 22060, Pakistan
2 Multan Institute of Nuclear Medicine and Radiotherapy, 377, Nishtar Hospital Multan, Pakistan
3 Department of Pharmacy, Bahauddin-Zakariya-University, Multan 60800, Pakistan
4 Sheikh Zayed Medical College, Rahim Yar Khan 64931, Pakistan
 

The present study was carried out to evaluate 28 medicinal plants of Pakistan having folklore claims to neutralize coagulopathy induced by Naja naja karachiensis bite in comparison with standard antidote. Venom was tested on citrated human plasma to determine its effect on prothrombin time (PT), activated partial thromboplastin time (aPTT) and thrombin time (TT). Snake venom (200 μg/ml) was found to delay PT (13 ± 0.57 to 23 ± 0.57 sec), aPTT (35 ± 1.52 to 48 ± 2.0 sec) and TT (13 ± 0.57 to 33 ± 0.57 sec) within 4.5% coefficient of variance. Prolongation of PT and TT suggested the presence of thrombin-like or plasminogen activating enzymes. Methanolic plant extracts (5 μg/ml) were considered as effective standard antidote. Enicostemma hyssopifolium (Willd.) Verdoorn (PT = 22 ± 0.57 sec, aPTT = 36 ± 1.00 sec, TT = 19 ± 0.57 sec) and Stenolobium stans (L) D. Don (PT = 16 ± 0.57 sec, aPTT = 36 ± 0.57 sec, TT = 29 ± 0.57 sec) were considered the most protective (≥70%, but ≤92%) from the rest of the listed medicinal plants. Nevertheless, further studies are required for identification and segregation of bioactive constituent(s) as an alternate and cheap source to treat anticoagulation.

Keywords

Antidote, Coagulopathy, Medicinal Plants, Naja naja karachiensis.
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  • Compensatory Effects of Medicinal Plants of Pakistan upon Prolongation of Coagulation Assays Induced by Naja naja karachiensis Bite

Abstract Views: 467  |  PDF Views: 134

Authors

Muhammad Hassham Hassan Bin Asad
Department of Pharmacy, COMSATS Institute of Information Technology, Abbottabad 22060, Pakistan
Durr-e-Sabih
Multan Institute of Nuclear Medicine and Radiotherapy, 377, Nishtar Hospital Multan, Pakistan
Bashir Ahmad Choudary
Department of Pharmacy, Bahauddin-Zakariya-University, Multan 60800, Pakistan
Arooj Fatima Asad
Sheikh Zayed Medical College, Rahim Yar Khan 64931, Pakistan
Ghulam Muratza
Department of Pharmacy, COMSATS Institute of Information Technology, Abbottabad 22060, Pakistan
Izhar Hussain
Department of Pharmacy, COMSATS Institute of Information Technology, Abbottabad 22060, Pakistan

Abstract


The present study was carried out to evaluate 28 medicinal plants of Pakistan having folklore claims to neutralize coagulopathy induced by Naja naja karachiensis bite in comparison with standard antidote. Venom was tested on citrated human plasma to determine its effect on prothrombin time (PT), activated partial thromboplastin time (aPTT) and thrombin time (TT). Snake venom (200 μg/ml) was found to delay PT (13 ± 0.57 to 23 ± 0.57 sec), aPTT (35 ± 1.52 to 48 ± 2.0 sec) and TT (13 ± 0.57 to 33 ± 0.57 sec) within 4.5% coefficient of variance. Prolongation of PT and TT suggested the presence of thrombin-like or plasminogen activating enzymes. Methanolic plant extracts (5 μg/ml) were considered as effective standard antidote. Enicostemma hyssopifolium (Willd.) Verdoorn (PT = 22 ± 0.57 sec, aPTT = 36 ± 1.00 sec, TT = 19 ± 0.57 sec) and Stenolobium stans (L) D. Don (PT = 16 ± 0.57 sec, aPTT = 36 ± 0.57 sec, TT = 29 ± 0.57 sec) were considered the most protective (≥70%, but ≤92%) from the rest of the listed medicinal plants. Nevertheless, further studies are required for identification and segregation of bioactive constituent(s) as an alternate and cheap source to treat anticoagulation.

Keywords


Antidote, Coagulopathy, Medicinal Plants, Naja naja karachiensis.



DOI: https://doi.org/10.18520/cs%2Fv106%2Fi6%2F870-873