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Palladium(II) Complex Induces Apoptosis through ROS-Mediated Mitochondrial Pathway in Human Lung Adenocarcinoma Cell Line (A549)


Affiliations
1 Immunology Lab, Department of Zoology, University of Calcutta, 35, Ballygunge Circular Road, Kolkata 700 019, India
2 Department of Environmental Science, University of Kalyani, Nadia 741 235, India
 

Treatment for lung cancer is still far from satisfactory. Therefore, there is a call for novel anticancer agents. In the present study, the anticancer activity of [Pd(L)Cl], 1a complex towards A549 human lung adenocarcinoma cells was investigated. [Pd(L)Cl], 1a inhibited the growth of A549 cells in a time and dosedependent manner. The IC50 value was 10 μM after 24 h treatment. Flow cytometric analyses revealed a dose-related increase in the percentages of cells in the Sub-G0/G1 state, indicative of apoptosis which was further confirmed by Annexin V binding assay, via a ROS-mediated mitochondria-dependent pathway. Western blot analysis showed that 1a complex induced Bax expression to desintegrate the outer mitochondrial membrane and causing cytochrome c release, associated with the activation of caspase-3. All of these signal transduction pathways are involved in initiating apoptosis. [Pd(L)Cl], 1a seems to represent a potentially active drug against non-small cell lung cancer A549 cell line in vitro, and further studies in vivo are warranted.

Keywords

Apoptosis, Lung Cancer, Palladium(II) Complexes, Reactive Oxygen Species.
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  • Palladium(II) Complex Induces Apoptosis through ROS-Mediated Mitochondrial Pathway in Human Lung Adenocarcinoma Cell Line (A549)

Abstract Views: 222  |  PDF Views: 87

Authors

Tarun Keswani
Immunology Lab, Department of Zoology, University of Calcutta, 35, Ballygunge Circular Road, Kolkata 700 019, India
Santanu Chowdhury
Department of Environmental Science, University of Kalyani, Nadia 741 235, India
Soma Mukherjee
Department of Environmental Science, University of Kalyani, Nadia 741 235, India
Arindam Bhattacharyya
Immunology Lab, Department of Zoology, University of Calcutta, 35, Ballygunge Circular Road, Kolkata 700 019, India

Abstract


Treatment for lung cancer is still far from satisfactory. Therefore, there is a call for novel anticancer agents. In the present study, the anticancer activity of [Pd(L)Cl], 1a complex towards A549 human lung adenocarcinoma cells was investigated. [Pd(L)Cl], 1a inhibited the growth of A549 cells in a time and dosedependent manner. The IC50 value was 10 μM after 24 h treatment. Flow cytometric analyses revealed a dose-related increase in the percentages of cells in the Sub-G0/G1 state, indicative of apoptosis which was further confirmed by Annexin V binding assay, via a ROS-mediated mitochondria-dependent pathway. Western blot analysis showed that 1a complex induced Bax expression to desintegrate the outer mitochondrial membrane and causing cytochrome c release, associated with the activation of caspase-3. All of these signal transduction pathways are involved in initiating apoptosis. [Pd(L)Cl], 1a seems to represent a potentially active drug against non-small cell lung cancer A549 cell line in vitro, and further studies in vivo are warranted.

Keywords


Apoptosis, Lung Cancer, Palladium(II) Complexes, Reactive Oxygen Species.



DOI: https://doi.org/10.18520/cs%2Fv107%2Fi10%2F1711-1719