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Batjuka, A.
- Evaluation of Superoxide Anion Level and Membrane Permeability in the Functionally Different Organs of Triticum aestivum L. Exposed to High Temperature and Antimycin A
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PDF Views:77
Authors
A. Batjuka
1,
N. Skute
1
Affiliations
1 Laboratory of Molecular Biology and Genetics, Department of Ecology, Institute of Life Sciences and Technology, Daugavpils University, Parades Str.1a, Daugavpils, Latvia, LV-5401, LV
1 Laboratory of Molecular Biology and Genetics, Department of Ecology, Institute of Life Sciences and Technology, Daugavpils University, Parades Str.1a, Daugavpils, Latvia, LV-5401, LV
Source
Current Science, Vol 117, No 3 (2019), Pagination: 440-447Abstract
The impact of antimycin A (AA) as an activator of the alternative respiratory pathway (AP) on the rate of superoxide anion (O•2¯) generation and membrane permeability was studied in the functionally different organs of wheat seedlings (Triticum aestivum L.) following short-term and long-term high temperature (HT). The results indicated a significant increase in the O•2¯ generating rate in etiolated wheat seedlings and seedlings grown under normal daylight regime following short-term (8%–37%) and long-term (13%– 65%) HT depending on the stage of development. The damages induced by HT were related to the permeability of membranes in the functionally different organs of wheat seedlings. However, our results demonstrated that increase in the O•2¯ generating rate in etiolated wheat seedlings was significantly lower following the combined effect of AA and HT, than under stressful conditions without AA. Taken together, our observations show that the induction of AP, provoked by AA, prevents over-reduction of the mitochondrial electron transport chain, alleviating reactive oxygen species formation and protecting the etiolated wheat seedlings against oxidative damage.Keywords
Antimycin A, High Temperature, Membrane Permeability, Superoxide Anion, Triticum aestivum L.Full Text
References
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- Cyclical Assessment of Superoxide Anion-Radical Generation and Characterization of Linking Physiological Parameters in Wheat (Triticum aestivum L.) Seedlings
Abstract Views :161 |
PDF Views:75
Authors
Affiliations
1 Laboratory of Molecular Biology and Genetics, Ecology Department, Institute of Life Sciences and Technology, Daugavpils University, Parades Str. 1a, Daugavpils, LV
1 Laboratory of Molecular Biology and Genetics, Ecology Department, Institute of Life Sciences and Technology, Daugavpils University, Parades Str. 1a, Daugavpils, LV
Source
Current Science, Vol 122, No 1 (2022), Pagination: 93-98Abstract
This study was conducted to evaluate the dynamics of superoxide anion radical (O2·–) production rate and alterations in DNA content and permeability of cell membranes in etiolated wheat seedlings (Triticum aestivum L. cv. Harmony) as well as those grown under normal daylight regime. The results suggest that the development of etiolated wheat seedlings and those grown under normal daylight is accompanied by the periodic formation of O2·– which leads to alterations in DNA content as well as the permeability of cell membranes. The results also indicate that the first enhancement in the rate of O2·– generation was detected on the sixth and seventh days of development, after which the rate of Oˉ production reduced in etiolated seedlings and those grown under normal daylight regime. The second maximum of the O2·– producing rate in developing and senescent organs occurred on the ninth day of plant development. The lowest values of all studied parameters, such as O2·– producing rate (37%), total genomic DNA concentration (58%) and electrolyte leakage (EL; 18%) were observed in etiolated wheat seedlings. Towards the end of the study period, DNA concentration and EL in organs of wheat seedlings declined, suggesting possible destruction of cellular organelles and the beginning of apoptotic processes. Overall, these results indicate that O– generation is decisive for normal morphogenesis, and it is an indispensable element of synchronous growth and development.Keywords
DNA Synthesis, Membrane Permeability, Physiological Parameters, Superoxide Radical, Triticum aestivum L.Full Text
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