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Daginawala, Hatim F.
- Evaluation of Peptide-Based Approach for Estimation of Nse and S-100ββ towards the Development of a Cost-Effective Test for Prognosis of AIS Patients
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PDF Views:112
Authors
Amit R. Nayak
1,
Neha H. Lande
1,
Anuja P. Kawle
1,
Dinesh P. Kabra
1,
Nitin H. Chandak
1,
Hemant J. Purohit
2,
Girdhar M. Taori
1,
Hatim F. Daginawala
1,
Rajpal S. Kashyap
1
Affiliations
1 Biochemistry Research Centre, Central India Institute of Medical Sciences, Nagpur 440 020, IN
2 Environmental Genomics Unit, National Environmental Engineering Research Institute, Nehru Marg, Nagpur 440 020, IN
1 Biochemistry Research Centre, Central India Institute of Medical Sciences, Nagpur 440 020, IN
2 Environmental Genomics Unit, National Environmental Engineering Research Institute, Nehru Marg, Nagpur 440 020, IN
Source
Current Science, Vol 109, No 8 (2015), Pagination: 1466-1470Abstract
Here we compare the in-house ELISA technique for estimation of neuron-specific enolase (NSE) and S-100ββ (glial-specific protein) peptides with a commercial kit in serum samples of acute ischaemic stroke (AIS) patients. Nineteen improved and four expired AIS patients were included for the study. NSE concentrations were significantly higher (P < 0.05) in expired as compared with improved AIS patients by both inhouse ELISA and commercial kit method. Estimation of S-100ββ by only in-house ELISA showed significantly high (P < 0.05) levels in expired AIS patients. Peptide-based estimation of NSE and S-100ββ may be used for prognosis of AIS patients.Keywords
Acute Ischaemic Stroke, Glial-Specific Protein, Neurological Disease, Neuron-specific Enolase, Prognosis.Full Text
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