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Rajasabapathy, Raju
- Culturable Bacterial Phylogeny from a Shallow Water Hydrothermal Vent of Espalamaca (Faial, Azores) Reveals a Variety of Novel Taxa
Abstract Views :244 |
PDF Views:84
Authors
Raju Rajasabapathy
1,
Chellandi Mohandass
1,
Ana Colaco
2,
Syed Gulam Dastager
3,
Ricardo Serrao Santos
2,
Ram Murti Meena
1
Affiliations
1 Biological Oceanography Division, CSIR-National Institute of Oceanography, Dona Paula, Goa 403 004, IN
2 IMAR-Department of Oceanography and Fisheries, University Açores, Cais de Sta Cruz, 9901-862, Horta, PT
3 NCIM Resource Center, CSIR-National Chemical Laboratory, Dr Homi Bhabha Road, Pune 411 008, IN
1 Biological Oceanography Division, CSIR-National Institute of Oceanography, Dona Paula, Goa 403 004, IN
2 IMAR-Department of Oceanography and Fisheries, University Açores, Cais de Sta Cruz, 9901-862, Horta, PT
3 NCIM Resource Center, CSIR-National Chemical Laboratory, Dr Homi Bhabha Road, Pune 411 008, IN
Source
Current Science, Vol 106, No 1 (2014), Pagination: 58-69Abstract
Phylogenetic diversity of the 16S rRNA gene asso-ciated with the domain bacteria was examined at the level of operational taxonomic units (OTUs) using the rarefaction analysis from a newly identified shallow water hydrothermal vent, Espalamaca in the Azorean Island Faial (Horta), North Atlantic Ocean. Diluted sea water nutrient agar amended with metals manga-nese, lead, iron and element sulphur, could help in re-trieving highest OTUs (95) from the vent and 39 OTUs from nonvent. Molecular tools implemented on bac-terial census indicated the occupancy of γ-Proteobacteria by 55.78%, α-Proteobacteria by 21.05% and 12.63% of Bacteroidetes in the total population. Occurrence of novel species maximized with α-Proteobacteria (11/20) followed by Bacteroi-detes (5/12) signified the necessity of studying these groups to strengthen the biodiversity database. Shan-non index (H') and the Chao I richness estimator illu-strated a strong bacterial community in the venting area. The current study confers many bacterial genera which were not reported earlier in any of the shallow water vents and adds 33 new taxa to the database.Keywords
Bacterial Phylogeny, Metals, Novel Taxa, Rarefaction Analysis, Shallow Vent.Full Text
- Bacterial Diversity at a Shallow-Water Hydrothermal Vent (Espalamaca) in Azores Island
Abstract Views :218 |
PDF Views:75
Authors
Raju Rajasabapathy
1,
Chellandi Mohandass
1,
Raul Bettencourt
2,
Ana Colaço
2,
Joana Goulart
2,
Ram Murti Meena
1
Affiliations
1 Biological Oceanography Division, CSIR-National Institute of Oceanography, Dona Paula, Goa 403 004, IN
2 IMAR-Department of Oceanography and Fisheries, University of Azores, Rua Prof Frederico Machado 9901-862, Horta, PT
1 Biological Oceanography Division, CSIR-National Institute of Oceanography, Dona Paula, Goa 403 004, IN
2 IMAR-Department of Oceanography and Fisheries, University of Azores, Rua Prof Frederico Machado 9901-862, Horta, PT
Source
Current Science, Vol 115, No 11 (2018), Pagination: 2110-2121Abstract
A low-temperature shallow-water hydrothermal vent field was discovered during the summer of 2010 in the Faial-Pico channel off the Espalamaca headland, Faial Island, Azores, Portugal, NE Atlantic. The present study analyses bacterial communities present in shallowwater hydrothermal vent of Espalamaca using SSU rRNA-based clone library approach. Clones of shallow vent sediment sample revealed the dominance of Proteobacteria (including α, γ, ε, δ, ζ subdivisions) and Bacteroidetes with 36% and 28% of the whole community respectively. The dominance of γ-Proteobacteria is the unique characteristic of this shallow vent and it coincides with the South Tonga Arc and Bahía Concepción (Pacific Ocean), whereas ε- Proteobacteria groups were reported to be high in the majority of the hydrothermal vents. Though the sampling sites of the venting and non-venting regions of Espalamaca were only 500 m apart, high variation (>80%) of phylotypes was found between the regions.Keywords
Bacterial Diversity, Clone Library, Hydrothermal Vent, Phylogeny, Shallow Water.Full Text
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