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Oral Administration of Root Extract of Boerhaavia diffusa Mitigates Diabetes-induced Kidney Damages in the Golden Hamster Mesocricetus auratus .


Affiliations
1 Pineal Research Laboratory, Department of Zoology, Institute of Science, Banaras Hindu University, Varanasi – 221005, Uttar Pradesh, India ., India
2 Department of Dravyagun, Institute of Medical Science, Banaras Hindu University, Varanasi – 221005, Uttar Pradesh, India ., India
3 Kalinga Institute of Social Sciences-Deemed University, Bhubaneshwar – 751024, Odisha, India ., India
     

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Diabetes, a common metabolic disorder, is affecting people irrespective of age group and/or gender. The chronic hyperglycemia during diabetes leads to heart-, kidney-, eye- and nerve damages. Boerhaavia diffusa, commonly known as Punarnava, is one of the traditional medicines described in Ayurveda for the treatment of a number of diseases. B. diffusa has been reported to exhibit antidiabetic, diuretic, anti-inflammatory, hepatoprotective, and immunomodulatory properties. The present study was carried out to evaluate the anti-hyperglycemic potential of ethanolic extract of root of B. diffusa and its effect on diabetes-induced kidney damage in hamster model. Treatment of ethanolic extract of B. diffusa resulted in significant reduction in the serum glucose level and increased insulin concentration with a simultaneous increment in the levels of muscle and liver glycogen. The oral supplementation of B. diffusa root extract improved lipid profile by reducing the cholesterol (TC), low density lipoprotein cholesterol (LDL-C) and by increasing high density lipoprotein cholesterol (HDL-C). The activity of antioxidant enzymes Superoxide Dismutase (SOD) and Catalase (CAT) in the kidney significantly improved following oral administration of B. diffusa while a significant decrease in Lipid Peroxidation (LPO) level was noted in the kidney after the treatment. Ethanolic root extract of B. diffusa decreased the level of serum creatinine, urea and Alkaline Phosphatase (ALP) significantly. B. diffusa administration demonstrated marked improvement in renal histology as evident by the regenerative changes in glomerulus and Bowman’s capsule. Thus, the use of ethanolic extract of Boerhaavia diffusa may prove therapeutically effective in prevention as well as inhibition of the progression of diabetes and associated kidney damages

Keywords

Boerhaavia diffusa, Diabetes, Diabetic Nephropathy, Hamsters, Punarnava .
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  • Arora S, Haldar C. Diabetes: Rescue by Boerhaavia diffusa. Journal of Endocrinology and Reproduction. 2018; 22(2):1-3.

  • Alam P, Shahzad N, Gupta AK, Mahfoz AM, Bamagous GA, Al-Ghamdi SS, Siddiqui NA. Anti-diabetic effect of Boerhavia diffusa L. root extract via free radical scavenging and antioxidant mechanism. Toxicology and Environmental Health Sciences. 2018; 10(3):220-227. https://doi.org/10.1007/s13530-018-0367-z

  • Chauhan SK, Thapliyal RP, Ojha SK, Rai H, Singh P, Singh M. Antidiabetic and antioxidant effect of ethanolic root extract of Boerhaavia diffusa in streptozotocin-induced diabetic rats. Journal of Pharmacy Research. 2011; 4(2):446-448.

  • Malhotra D, Ishaq F, Khan A. Antihyperglycemic activity of Boerhaavia diffusa in streptozotocin induced diabetic rats. International Journal of Chemical and Analytical Sciences. 2014; 5(1):21-23.

  • Satheesh MA, Pari L. Antioxidant effect of Boerhavia diffusa L. in tissues of alloxan induced diabetic rats. Indian Journal Experimental Biology. 2004; 42(10):989-992. https://doi.org/10.1300/J044v10n04_11

  • Nalamolu RK, Boini KM, Nammi S. Effect of chronic administration of Boerhaavia diffusa Linn. leaf extract on experimental diabetes in rats. Tropical Journal of Pharmaceutical Research. 2004; 3(1):305-309. https://doi.org/10.4314/tjpr.v3i1.14614

  • Murti K, Lambole V, Panchal M, Kumar U. Antidiabetic and antihyperlipidemic activity of roots of Boerhaavia diffusa on streptozotocin induced diabetic rats. Pharmacologyonline. 2011; 1:15-21.

  • Pareta SK, Patra KC, Mazumder PM, Sasmal D. Aqueous extract of Boerhaavia diffusa root ameliorates ethylene glycol-induced hyperoxaluric oxidative stress and renal injury in rat kidney. Pharmaceutical Biology. 2011; 49(12): 1224-1233. https://doi.org/10 .3109/13880209.2011.581671 PMid:21846174

  • Sawardekar SB, Patel TC. Evaluation of the effect of Boerhavia diffusa on gentamicin-induced nephrotoxicity in rats. Journal of Ayurveda and integrative medicine. 2015; 6(2):95-103. https://doi.org/10.4103/0975-9476.146545 PMid:26166999 PMCid:PMC4484055

  • Indhumathi T, Shilpa K, Mohandass S. Evaluation of nephroprotective role of Boerhaavia diffusa leaves against mercuric chloride induced toxicity in experimental rats. Journal of Pharmacy Research. 2011; 4(6):1848-1850.

  • Pareta SK, Patra KC, Harwansh R, Kumar M, Meena KP. Protective effects of Boerhaavia diffusa against acetaminophen-induced nephrotoxicity in rats. PharmacologyOnline. 2011; 2:698-706 (b).

  • Oburai NL, Rao VV, Bonath RB. Comparative clinical evaluation of Boerhavia diffusa root extract with standard Enalapril treatment in Canine chronic renal failure. Journal of Ayurveda and integrative medicine. 2015; 6(3):150-157. https://doi.org/10.4103/09759476.166390 PMid:26604549 PMCid:PMC4630688

  • Akhter F, Alvi SS, Ahmad P, Iqbal D, Alshehri BM, Khan MS. Therapeutic efficacy of Boerhaavia diffusa (Linn.) root methanolic extract in attenuating streptozotocin-induced diabetes, diabetes-linked hyperlipidemia and oxidative-stress in rats. Biomedical research and therapy. 2019; 6(7):3293-3306. https://doi.org/10.15419/bmrat.v6i7.556

  • Mandal S, Bapna JS. Pharmacological evaluation of Boerhavia diffusa plant extract for their potential in management of diabetic nephropathy in streptozotocin induced type diabetic rats. World Journal of Pharmacy and Pharmaceutical Sciences. 2016; 5(12): 1097-1108.

  • Singh PK, Baxi D, Doshi A, Ramachandran AV. Antihyperglycaemic and renoprotective effect of Boerhaavia diffusa L. in experimental diabetic rats. Journal of Complementary and Integrative Medicine. 2011; 8:(1). https://doi.org/10.2202/15533840.1533 PMid:22754925

  • Motawi TK, Ahmed SA, Hamed MA, El-Maraghy SA, Aziz WM. Combination of melatonin and certain drugs for treatment of diabetic nephropathy in streptozotocin-induced diabetes in rats. Diabetology International. 2016; 7(4):413-424. https://doi. org/10.1007/s13340-016-0268-9 PMid:30603294 PMCid:PMC6224965

  • Das K, Samanta L, Chainy GBN. A modified spectrophotometric assay of superoxide dismutase using nitrite formation by superoxide radicals. Indian Journal of Expeerimental Biology 2000; 37(3):201-204

  • Sinha AK. Colorimetric assay of catalase. Analytical Biochemistry. 1972; 47(2):389-394. https://doi.org/10.1016/0003- 2697(72)90132-7

  • Ohkawa H, Ohishi N, Yagi K. Reaction of linoleic acid hydroperoxide with thiobarbituric acid. Journal of Lipid Research. 1978; 19(8):1053-1057. https://doi.org/10.1016/S0022-2275(20)40690-X

  • Jasmine R. Restoration of liver and muscle glycogen by Eugenia jambolina in diabetic rats. International Journal of Research in Pharmacy and Biosciences. 2014; 1(2):16-21. https://doi.org/10.17812/IJPER/2014;1(3):16-21

  • Shajeela PS, Kalpanadevi V, Mohan VR. Potential antidiabetic, hypo lipidaemic and antioxidant effects of Nymphaea pubescens extract in alloxan induced diabetic rats. J Appl Pharmaceut. Sci. 2012;2(2):83-88.

  • Soliman AM. Potential impact of Paracentrotus lividus extract on diabetic rat models induced by high fat diet/streptozotocin. Journal of Basic and Applied Zoology 2016; 77:8-20. https://doi.org/10.1016/j.jobaz.2016.01.001

  • Mathur A, Mathur R. Study of association of serum lipids with diabetic retinopathy in type 2 diabetes mellitus. People’s Journal of Scientific Research 2013; 6(1):25-28.

  • Leighton E, Sainsbury CA, Jones GC. A practical review of C-peptide testing in diabetes. Diabetes Therapy 2017; 8(3):475-487. https://doi.org/10.1007/s13300-017-0265-4 PMid:28484968 PMCid:PMC5446389

  • Gu Q, Feng T, Cao H. Tang Y, Ge X, Luo J, Xue J, Wu J, Yang H, Zhang S, Cao J. HIV-TAT mediated protein transduction of Cu/Zn-superoxide dismutase-1(SOD-1) protects skin cells from ionizing radiation. Radiation Oncology 2013; 8:253.https://doi. org/10.1186/1748-717X-8-253 PMid:24175971 PMCid:PMC3839640

  • Kumar G, Banu GS, Murugesan AG, Pandian MR. Antihyperglycemic and antiperoxidative effect of Heicteres igora l. bark extracts in streptozotocin-induced diabetic rats. J Appl Biomed. 2007; 5:97-104. https://doi.org/10.32725/jab.2007.014

  • Tian W, Lei H, Guan R, Xu Y, Li H, Wang L, Yang B, Gao Z, Xin Z. Icariside II ameliorates diabetic nephropathy in streptozotocin-induced rats. Drug Des Devel Ther. 2015; 9:5147-5157. https://doi.org/10.2147/DDDT.S90060 PMid:26379427 PMCid:PMC4567177

  • Zhang S, Xu H, Yu X, Wang Y, Sun F, Sui D. Simvastatin ameliorates low-dose streptozotocin-induced type 2 diabetic nephropathy in an experimental rat model. Int J Clin Exp Med. 2015; 8(4):6388-6396.

  • Veeramani C, Pushpavalli G, Pugalendi KV. Antihyperglycemic effect of Cardiospermum halicacabum Linn. leaf extrac on streptozotocin induced diabetic rats. J Appl Biomed. 2008; 6:19-26. https://doi.org/10.32725/jab.2008.003

  • Sharma A, Hirulkar NB, Wadel P, Da P. Detection of renal function parameters urea and creatinine. Int J Pharmaceut Biol Arch. 2011; 2(2):734-739.

  • Gowda S, Desai PB, Kulkarni SS, Hull VV, Math KA, Vernekar SN. Markers of renal function tests. N Am J Med Sci. 2010; 2(4):170-173.

  • Kaleem M, Medha Ahmed QU, Asif M, Bano B. Benaficial effects of Annona squamosa extract in streptozotocin-induced diabetic rats. Singapore Med. J. 2008; 49(10):800-804.

  • Alibawi FN, Al-Morshidy SY, Alhuweizi AG. The Alkaline Phosphatase Levels in the Seminal Plasma and Sperms of Sub-fertile Patients and Normospermic Men. InInternational Conference on Applied Life Sciences. 2012 Sep 10. IntechOpen. .


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  • Oral Administration of Root Extract of Boerhaavia diffusa Mitigates Diabetes-induced Kidney Damages in the Golden Hamster Mesocricetus auratus .

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Authors

Sweta Arora
Pineal Research Laboratory, Department of Zoology, Institute of Science, Banaras Hindu University, Varanasi – 221005, Uttar Pradesh, India ., India
Sweta Arora
Department of Dravyagun, Institute of Medical Science, Banaras Hindu University, Varanasi – 221005, Uttar Pradesh, India ., India
Chandana Haldar
Pineal Research Laboratory, Department of Zoology, Institute of Science, Banaras Hindu University, Varanasi – 221005, Uttar Pradesh, India ., India
Priyoneel Basu
Kalinga Institute of Social Sciences-Deemed University, Bhubaneshwar – 751024, Odisha, India ., India
Jasmeet Singh
Department of Dravyagun, Institute of Medical Science, Banaras Hindu University, Varanasi – 221005, Uttar Pradesh, India ., India
Sameer Gupta
Pineal Research Laboratory, Department of Zoology, Institute of Science, Banaras Hindu University, Varanasi – 221005, Uttar Pradesh, India ., India

Abstract


Diabetes, a common metabolic disorder, is affecting people irrespective of age group and/or gender. The chronic hyperglycemia during diabetes leads to heart-, kidney-, eye- and nerve damages. Boerhaavia diffusa, commonly known as Punarnava, is one of the traditional medicines described in Ayurveda for the treatment of a number of diseases. B. diffusa has been reported to exhibit antidiabetic, diuretic, anti-inflammatory, hepatoprotective, and immunomodulatory properties. The present study was carried out to evaluate the anti-hyperglycemic potential of ethanolic extract of root of B. diffusa and its effect on diabetes-induced kidney damage in hamster model. Treatment of ethanolic extract of B. diffusa resulted in significant reduction in the serum glucose level and increased insulin concentration with a simultaneous increment in the levels of muscle and liver glycogen. The oral supplementation of B. diffusa root extract improved lipid profile by reducing the cholesterol (TC), low density lipoprotein cholesterol (LDL-C) and by increasing high density lipoprotein cholesterol (HDL-C). The activity of antioxidant enzymes Superoxide Dismutase (SOD) and Catalase (CAT) in the kidney significantly improved following oral administration of B. diffusa while a significant decrease in Lipid Peroxidation (LPO) level was noted in the kidney after the treatment. Ethanolic root extract of B. diffusa decreased the level of serum creatinine, urea and Alkaline Phosphatase (ALP) significantly. B. diffusa administration demonstrated marked improvement in renal histology as evident by the regenerative changes in glomerulus and Bowman’s capsule. Thus, the use of ethanolic extract of Boerhaavia diffusa may prove therapeutically effective in prevention as well as inhibition of the progression of diabetes and associated kidney damages

Keywords


Boerhaavia diffusa, Diabetes, Diabetic Nephropathy, Hamsters, Punarnava .

References