Study of Hypothetical Proteins in Salmonella Phages and Predicting their Structural and Functional Relationship

The present study of hypothetical proteins in Salmonella phages was carried out for predicting their structure and function relationship. Such applications for bacteriophages are not yet reported and there is a great need to understand the mechanism by which they impart the virulence, resistance and other novel characteristics to the host they infect. We are reporting the structure and functions of hypothetical proteins in Salmonella phages for understanding their role in Salmonella spp. The complete analysis of hypothetical proteins from 8 different Salmonella phage genomes were carried out by the using bioinformatics web tools like CDD-BLAST, INTERPROSCAN, PFAM and COGs by searching orthologous enzymatic conserved domains in the hypothetical sequences. While tertiary structures were constructed using PS2 Server (Protein Structure Prediction server). These studies revealed presences of enzymatic functional domain in 110 uncharacterized proteins and 3-D protein structure were predicted for 29 hypothetical proteins. These deciphered enzymatic data for hypothetical proteins can be used for the understanding of functional, structural, and evolutionary development of Salmonella phages and its life cycle along with their role in host evolution and pathogenicity.