Mathematical Modeling of Intrinsic Raman Spectroscopy for Biological Applications

Intrinsic Raman spectroscopy (IRS) is a technique to correct turbidity-induced Raman spectral distortions, resulting in the intrinsic Raman spectrum that would be observed in the absence of scattering and absorption. In this paper, we develop a closed form expression relating the observed and intrinsic Raman spectra through diffuse reflectance using a numerical technique. Also, we study the dependence of this expression on sample size and elastic scattering anisotropy. We compare the behavior   at various turbidities, at various collection spot radii, for different sample sizes and for various values of elastic scattering anisotropy () to get best fitting with minimum error and to have an accurate relation between observed and intrinsic Raman spectra.