Functionality Search in Hypothetical Proteins of Halobacterium Salinarum

The Halobacterium salinarum are extremely halophilic archaeon. The complete genome sequence of Halobacterium salinarum was deciphered, which revealed the presence of various gene for hypothetical proteins whose function are not yet understood. We analyzed complete genome of Halobacterium salinarum for hypothetical proteins from which 524 uncharacterized proteins showed conserved domains for biologically active components. Our attempt is to predict the function of these hypothetical proteins by the application of computational methods and Bioinformatics. The probable function prediction of the hypothetical protein was done by using Bioinformatics web tools like CDD-BLAST, INTERPROSCAN, PFAM and COGs by searching sequence databases for the presence of orthologous enzymatic conserved domains in the hypothetical sequences. These study revealed presences of enzymatic functional domain in Halobacterium salinarum; their roles are yet to be discovered. These deciphered enzymatic data for hypothetical proteins can be used for the understanding of functional, structural, evolutionary and metabolic development of Halobacterium spp. and its life cycle along with their role in host evolution and adaptation.