Himachal Journal of Agricultural Research

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Detection of Haemoprotozoa in Cows and Buffaloes in Kangra District of Himachal Pradesh


Affiliations
1 Department of Veterinary Microbiology, DGCN College of Veterinary and Animal Sciences, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur - 176 062, India
 

In this study, a total of 57 blood samples from cows and buffaloes showing clinical signs of high fever, anemia and/or swollen lymph nodeswere screened for haemoprotozoan infections using polymerase chain reaction (PCR). Some asymptomatic animals were also included in the study. Out of 57 blood samples, 14 samples were positive for either one or the other haemoprotozoa reflecting an overall prevalence of 24.56%. A total of nine samples of which six from cows and three from buffaloes were positive for Theileria annulata and five samples all from cows were found positive for mixed infection of Babesia bovis and Anaplasma marginale. This study shows that haemoprotozoan infections are common in cows and buffaloes population of Kangra District of Himachal Pradeshdue to conduciveenvironmental conditions for survival of vectors responsible for carrying these infectious agents. PCR technique showed high sensitivity and specificityup to species level, as it not only detected the haemoprotozoan infections in active cases but also in asymptomatic carrier animals.

Keywords

Anaplasma marginale, Babesia bovis, Bovine Haemoprotozoan, Himachal Pradesh, Kangra, PCR.
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  • Detection of Haemoprotozoa in Cows and Buffaloes in Kangra District of Himachal Pradesh

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Authors

Jyoti Jaryal
Department of Veterinary Microbiology, DGCN College of Veterinary and Animal Sciences, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur - 176 062, India
Ajay Katoch
Department of Veterinary Microbiology, DGCN College of Veterinary and Animal Sciences, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur - 176 062, India
Sanjeev Kumar
Department of Veterinary Microbiology, DGCN College of Veterinary and Animal Sciences, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur - 176 062, India
Kritika Dhial
Department of Veterinary Microbiology, DGCN College of Veterinary and Animal Sciences, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur - 176 062, India
Mandeep Sharma
Department of Veterinary Microbiology, DGCN College of Veterinary and Animal Sciences, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur - 176 062, India
Prasenjit Dhar
Department of Veterinary Microbiology, DGCN College of Veterinary and Animal Sciences, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur - 176 062, India
Rajesh Chahota
Department of Veterinary Microbiology, DGCN College of Veterinary and Animal Sciences, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur - 176 062, India
Subhash Verma
Department of Veterinary Microbiology, DGCN College of Veterinary and Animal Sciences, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur - 176 062, India

Abstract


In this study, a total of 57 blood samples from cows and buffaloes showing clinical signs of high fever, anemia and/or swollen lymph nodeswere screened for haemoprotozoan infections using polymerase chain reaction (PCR). Some asymptomatic animals were also included in the study. Out of 57 blood samples, 14 samples were positive for either one or the other haemoprotozoa reflecting an overall prevalence of 24.56%. A total of nine samples of which six from cows and three from buffaloes were positive for Theileria annulata and five samples all from cows were found positive for mixed infection of Babesia bovis and Anaplasma marginale. This study shows that haemoprotozoan infections are common in cows and buffaloes population of Kangra District of Himachal Pradeshdue to conduciveenvironmental conditions for survival of vectors responsible for carrying these infectious agents. PCR technique showed high sensitivity and specificityup to species level, as it not only detected the haemoprotozoan infections in active cases but also in asymptomatic carrier animals.

Keywords


Anaplasma marginale, Babesia bovis, Bovine Haemoprotozoan, Himachal Pradesh, Kangra, PCR.

References