Himachal Journal of Agricultural Research

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Effects of Different Growth Media on Germinability of Interspecific Hybrids between Vigna mungo< and V. umbellata


Affiliations
1 Department of Genetics and Plant Breeding, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur-176 062, India
 

A total of 15 interspecific crosses involving five genotypes of urdbean (Vigna mungo) and three genotypes of ricebean (V. umbellata) were attempted to study the effects of different growth media on hybrid germination. Since the seeds obtained from the interspecific crosses did not germinate under normal soil conditions so attempts were made to grow them on various media i.e. Salt solution, Half and full strength MS medium, MS basal medium and Gamborg B5 media. Seeds were able to germinate only on the salt solution. F1 seeds obtained from cross Him Mash-1 x VRB-3 showed maximum response on salt solution with respect to radicle formation (44.60 per cent) followed by Palampur-93 x PRR-2 (38.30 per cent) & Him Mash-1 x PRR-1 (23.89 per cent).Cross Palampur-93 x PRR-2 (17.02 per cent) produced maximum number of plantlets followed by Him Mash-1 x VRB-3 (15.32 per cent). The study indicated that different kinds of post fertilization barriers such as retarded embryo & endosperm development, production of inviable & shriveled seed, inability of seed to form radicle and yellowing of plantlets are responsible for complete lethality. The crosses showing substantially high per cent of germination can be utilized for genetic improvement of urdbean.

Keywords

Interspecific Hybridization, Germinability, V. mungo, V. umbellata.
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  • Effects of Different Growth Media on Germinability of Interspecific Hybrids between Vigna mungo< and V. umbellata

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Authors

Shailja Sharma
Department of Genetics and Plant Breeding, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur-176 062, India
R. K. Mittal
Department of Genetics and Plant Breeding, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur-176 062, India
V. K. Sood
Department of Genetics and Plant Breeding, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur-176 062, India
H. K. Chaudhary
Department of Genetics and Plant Breeding, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur-176 062, India

Abstract


A total of 15 interspecific crosses involving five genotypes of urdbean (Vigna mungo) and three genotypes of ricebean (V. umbellata) were attempted to study the effects of different growth media on hybrid germination. Since the seeds obtained from the interspecific crosses did not germinate under normal soil conditions so attempts were made to grow them on various media i.e. Salt solution, Half and full strength MS medium, MS basal medium and Gamborg B5 media. Seeds were able to germinate only on the salt solution. F1 seeds obtained from cross Him Mash-1 x VRB-3 showed maximum response on salt solution with respect to radicle formation (44.60 per cent) followed by Palampur-93 x PRR-2 (38.30 per cent) & Him Mash-1 x PRR-1 (23.89 per cent).Cross Palampur-93 x PRR-2 (17.02 per cent) produced maximum number of plantlets followed by Him Mash-1 x VRB-3 (15.32 per cent). The study indicated that different kinds of post fertilization barriers such as retarded embryo & endosperm development, production of inviable & shriveled seed, inability of seed to form radicle and yellowing of plantlets are responsible for complete lethality. The crosses showing substantially high per cent of germination can be utilized for genetic improvement of urdbean.

Keywords


Interspecific Hybridization, Germinability, V. mungo, V. umbellata.

References