Himachal Journal of Agricultural Research

Open Access Open Access  Restricted Access Subscription Access

Isolation and Adaptational Studies of Native Sheeppox Virus Strains in Lamb Testicular and Vero Cell Culture Systems


Affiliations
1 Department of Veterinary Microbiology, Dr. G.C. Negi College of Veterinary and Animal Sciences, CSK Himachal Pradesh Krishi Vishwavidyalaya, Palampur-176 062, India
 

Sheeppox is a highly contagious disease of sheep causing huge economic losses to the sheep industry and farmers rearing the livestock. Himachal Pradesh has large population of sheep reared by the different rural communities. In recent years, outbreaks of sheep pox have become frequent causing huge economical losses to sheep breeders. Isolation, identification and characterization of circulating Sheeppox virus (SPV) strains is required to initiate efforts for disease control and prevention. The aim of the present study was to establish the best cell culture-based isolation system by comparing the growth of SPV field strains from Himachal Pradesh in lamb testicular (LT) cells and continuous Vero cell line. The results showed that LT cell culture is more suited for primary isolation of SPV field strains as compared to Vero cells. But virus can be adapted to Vero cell line after 5 blind passages. Thus, it was concluded that LT cell culture, though tedious, should be preferred for primary isolation of SPV field strains.

Keywords

Sheeppox Virus, LT Cell Culture, Vero Cell Line.
User
Notifications
Font Size

  • Aygün ST. 1959. The propagation of variola ovina in sheep embryonic tissue cultures and its usefulness as a vaccine against this disease. Archiv fur Experimentelle Veterinarmedizin 9.

  • Batta MK, Katoch RC, Mandeep S, Joshi VB and Nagal KB. 1999. Epidemiological observation on goatpox in Himachal Pradesh. Indian Veterinary Journal 76 (8):683-684.

  • Babiuk S, Parkyn G, Copps J, Larence JE, Sabara MI, Bowden TR, Boyle DB and Kitching RP. 2007. Evaluation of an ovine testis cell line (OA3. Ts) for propagation of Capripoxvirus isolates and development of an immunostaining technique for viral plaque visualization. Journal of Veterinary Diagnostic Investigation 19 (5):486-91.

  • Bhanuprakash V, BK Indrani, Hosamani, M and Singh RK. 2006. The current status of sheep pox disease. Comparative Immunology Microbiology and Infectious Disease 29 (1):27-60.

  • Boue A, Baltazard M and Vieuchange J. 1957. Culture du virus de la clavelée sur cultures de tissus 244 (11):15713.

  • Cilli, V, and Baldelli, B. 1959. Behaviour of sheeppox virus in tissue cultures of sheep testicular cells. Proc. XVIth Inter. veto Congr. 2:455.

  • Coakley W and Capstick PB. 1961. Protection of cattle against lumpy skin disease. Research in Veterinary Science12:123-7.

  • Hosamani M, Nandi S, Mondal B, Singh RK, Rasool TJ and Bandyopadhyay SK. 2004. A Vero cell-attenuated Goatpox virus provides protection against virulent virus challenge. Acta Virologica 48 (1):15-21.

  • Jassim FA, FA J. 1982. Propagation of sheeppox virus in cell culture. http://pascal.francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=PASCAL82X 0344425

  • King AMQ, Adams MJ, Carstens EB and Lefkowitz EJ. 2012. Virus Taxonomy – Ninth Report of the International Committee on Taxonomy of Viruses. Academic Press, San Diego, CA. pp291-309.

  • Kirubaharan JJ, Sugirtha AP and Padmanaban VD. 1993. Adaptation of Sheep Pox Virus (Ranipet Strain) to BHK-21 cell-line. Indian Veterinary Journal 70 (1):4.

  • Malik Y, Khatri M, Batra SK and Chand P. 1997. Occurrence of sheep pox in unorganized flocks in Haryana State. Indian Journal of Animal Sciences 67 (11):962-3.

  • Mateva V. 1976. Cultivation and studying the properties of the sheep pox virus, strain Perego, in a heterologous tissue. Veterinarnomedit þs!þinski nauki

  • Mirzaie K, Barani SM and Bokaie S. 2015. A review of sheep pox and goat pox: Perspective of their control and eradication in Iran. Journal of Advanced Veterinary Animal Research 2 (4):373-381.

  • Pandey R and Singh IP. 1970. Cytopathogenicity and neutralisation of sheep pox virus in primary cell culture of ovine and caprine origin. Indian Journal of Pathology and Bacteriology 13:6-11.

  • Plowright W and Ferris RD. 1958. The growth and cytopathogenicity of sheep-pox virus in tissue cultures. British Journal of Experimental Pathology 39 (4):424.

  • Ramyar H and Hessami M. 1967. Development of an attenuated live virus vaccine against sheep pox. Zentralblatt für Veterinärmedizin Reihe B 14 (6):516-519.

  • Sharma R, Patil RD, Parimoo HA, Thakur D and Katoch VC. 2013. Clinio-pathology of sheep pox disease in Himachal Pradesh, India. Ruminant Science 2(2):127-130.

  • -

  • Soman JP and Singh IP. 1980. Cytopathic and immunogenic studies of sheep pox virus serially cultivated in cell culture. Journal of Comparative Pathology 90 (1):99-106.

  • Trabelsi K, Majoul S, Rourou S and Kallel H. 2014. Process intensification for an enhanced replication of a newly adapted RM-65 sheep pox virus strain in Vero cells grown in stirred bioreactor. Biochemical Engineering Journal 90:131-9.

  • Verma S, Verma LK, Gupta VK, Katoch VC, Dogra V, Pal B and Sharma M. 2011. Emerging Capripoxvirus disease outbreaks in Himachal Pradesh, a Northern State of India. Transboundary and Emerging Diseases 58 (1):79-85.


Abstract Views: 76

PDF Views: 0




  • Isolation and Adaptational Studies of Native Sheeppox Virus Strains in Lamb Testicular and Vero Cell Culture Systems

Abstract Views: 76  |  PDF Views: 0

Authors

Tania Gupta
Department of Veterinary Microbiology, Dr. G.C. Negi College of Veterinary and Animal Sciences, CSK Himachal Pradesh Krishi Vishwavidyalaya, Palampur-176 062, India
Vanita Patial
Department of Veterinary Microbiology, Dr. G.C. Negi College of Veterinary and Animal Sciences, CSK Himachal Pradesh Krishi Vishwavidyalaya, Palampur-176 062, India
Prasenjit Dhar
Department of Veterinary Microbiology, Dr. G.C. Negi College of Veterinary and Animal Sciences, CSK Himachal Pradesh Krishi Vishwavidyalaya, Palampur-176 062, India
Subhash Verma
Department of Veterinary Microbiology, Dr. G.C. Negi College of Veterinary and Animal Sciences, CSK Himachal Pradesh Krishi Vishwavidyalaya, Palampur-176 062, India
Mandeep Sharma
Department of Veterinary Microbiology, Dr. G.C. Negi College of Veterinary and Animal Sciences, CSK Himachal Pradesh Krishi Vishwavidyalaya, Palampur-176 062, India
Rajesh Chahota
Department of Veterinary Microbiology, Dr. G.C. Negi College of Veterinary and Animal Sciences, CSK Himachal Pradesh Krishi Vishwavidyalaya, Palampur-176 062, India

Abstract


Sheeppox is a highly contagious disease of sheep causing huge economic losses to the sheep industry and farmers rearing the livestock. Himachal Pradesh has large population of sheep reared by the different rural communities. In recent years, outbreaks of sheep pox have become frequent causing huge economical losses to sheep breeders. Isolation, identification and characterization of circulating Sheeppox virus (SPV) strains is required to initiate efforts for disease control and prevention. The aim of the present study was to establish the best cell culture-based isolation system by comparing the growth of SPV field strains from Himachal Pradesh in lamb testicular (LT) cells and continuous Vero cell line. The results showed that LT cell culture is more suited for primary isolation of SPV field strains as compared to Vero cells. But virus can be adapted to Vero cell line after 5 blind passages. Thus, it was concluded that LT cell culture, though tedious, should be preferred for primary isolation of SPV field strains.

Keywords


Sheeppox Virus, LT Cell Culture, Vero Cell Line.

References