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Isolation and Adaptational Studies of Native Sheeppox Virus Strains in Lamb Testicular and Vero Cell Culture Systems
Sheeppox is a highly contagious disease of sheep causing huge economic losses to the sheep industry and farmers rearing the livestock. Himachal Pradesh has large population of sheep reared by the different rural communities. In recent years, outbreaks of sheep pox have become frequent causing huge economical losses to sheep breeders. Isolation, identification and characterization of circulating Sheeppox virus (SPV) strains is required to initiate efforts for disease control and prevention. The aim of the present study was to establish the best cell culture-based isolation system by comparing the growth of SPV field strains from Himachal Pradesh in lamb testicular (LT) cells and continuous Vero cell line. The results showed that LT cell culture is more suited for primary isolation of SPV field strains as compared to Vero cells. But virus can be adapted to Vero cell line after 5 blind passages. Thus, it was concluded that LT cell culture, though tedious, should be preferred for primary isolation of SPV field strains.
Keywords
Sheeppox Virus, LT Cell Culture, Vero Cell Line.
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